Rapid intraoperative Ki-67 immunohistochemistry for lung cancer using non-contact alternating current electric field mixing

Atari, Maiko; Imai, Kazuhiro; Nanjo, Hiroshi; Wakamatsu, Yoshiko; Takashima, Shinogu; Kurihara, Nobuyasu; Kuriyama, Sei; Suzuki, Haruka; Demura, Ryo; Harata, Yuzu; Hiroshima, Yuko; Sato, Yusuke; Nomura, Kyoko; Minamiya, Yoshihiro

doi:10.1016/j.lungcan.2022.09.007

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…Decision curve analysis was applied in this study to confirm the predictive value of the predictive model, and both internal and external validation were performed in this study to provide more insight into the generalizability of the combined model. It is widely acknowledged that the heterogeneity of tumor biology leads to varied prognoses reflecting the metastatic or invasive behavior of a particular tumor [ 8 ]. Therefore, this study aimed to avoid the impact of tumor heterogeneity on the accuracy of the predictive model.…”

Section: Discussionmentioning

confidence: 99%

“…Ki-67 expression is widely used in the diagnosis and treatment of malignant tumors to select surgical approaches, design targeted therapies, and evaluate prognosis [ 7 ]. A recent study demonstrated that rapid Ki-67 immunohistochemistry during surgery can be used to identify stage IA NSCLC patients with a greater risk of recurrence to aid in the selection of appropriate surgical strategies [ 8 ]. Additionally, this study suggested that segmentectomy may be a more reasonable choice than lobectomy for early-stage, low-grade NSCLC.…”

Section: Introductionmentioning

confidence: 99%

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Nomogram for the preoperative prediction of Ki-67 expression and prognosis in stage IA lung adenocarcinoma based on clinical and multi-slice spiral computed tomography features

Li,

Liu,

Wang

et al. 2024

BMC Med Imaging

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Objective This study developed and validated a nomogram utilizing clinical and multi-slice spiral computed tomography (MSCT) features for the preoperative prediction of Ki-67 expression in stage IA lung adenocarcinoma. Additionally, we assessed the predictive accuracy of Ki-67 expression levels, as determined by our model, in estimating the prognosis of stage IA lung adenocarcinoma. Materials and methods We retrospectively analyzed data from 395 patients with pathologically confirmed stage IA lung adenocarcinoma. A total of 322 patients were divided into training and internal validation groups at a 6:4 ratio, whereas the remaining 73 patients composed the external validation group. According to the pathological results, the patients were classified into high and low Ki-67 labeling index (LI) groups. Clinical and CT features were subjected to statistical analysis. The training group was used to construct a predictive model through logistic regression and to formulate a nomogram. The nomogram’s predictive ability and goodness-of-fit were assessed. Internal and external validations were performed, and clinical utility was evaluated. Finally, the recurrence-free survival (RFS) rates were compared. Results In the training group, sex, age, tumor density type, tumor-lung interface, lobulation, spiculation, pleural indentation, and maximum nodule diameter differed significantly between patients with high and low Ki-67 LI. Multivariate logistic regression analysis revealed that sex, tumor density, and maximum nodule diameter were significantly associated with high Ki-67 expression in stage IA lung adenocarcinoma. The calibration curves closely resembled the standard curves, indicating the excellent discrimination and accuracy of the model. Decision curve analysis revealed favorable clinical utility. Patients with a nomogram-predicted high Ki-67 LI exhibited worse RFS. Conclusion The nomogram utilizing clinical and CT features for the preoperative prediction of Ki-67 expression in stage IA lung adenocarcinoma demonstrated excellent performance, clinical utility, and prognostic significance, suggesting that this nomogram is a noninvasive personalized approach for the preoperative prediction of Ki-67 expression.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nomogram for the preoperative prediction of Ki-67 expression and prognosis in stage IA lung adenocarcinoma based on clinical and multi-slice spiral computed tomography features

Li,

Liu,

Wang

et al. 2024

BMC Med Imaging

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Octreotide modified liposomes that co-deliver paclitaxel and neferine effectively inhibit ovarian cancer metastasis by specifically binding to the SSTR2 receptors

Liu,

Wang,

et al. 2024

Journal of Drug Delivery Science and Technology

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Multiplex Intraoperative Rapid Immunohistochemistry with Noncontact Antibody Mixing for Distinguishing the Histologic Phenotype of Lung Cancer

Kuriyama,

Imai,

Nanjo

et al. 2024

Pathobiology

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Introduction: Determining a surgical strategy for early-stage lung cancer requires an accurate histologic diagnosis. Immunohistochemistry (IHC) enables reliable diagnosis of histological types but requires more time and more tumor tissue slides than hematoxylin and eosin staining. We aimed to assess the clinical validity of a new rapid multiplex IHC technique utilizing alternating current (AC) mixing for intraoperative lung cancer diagnosis. Methods: Forty-three patients who underwent radical resection of lung cancers were enrolled in a retrospective observational study. Frozen sections were prepared from lung tumor samples, and rapid IHC employing AC mixing was implemented alongside a multiplex IHC protocol targeting thyroid transcription factor-1 + cytokeratin 5, desmoglein 3 + Napsin A, and p63 + tripartite motif containing 29. We then evaluated the concordance between intraoperative diagnoses derived from rapid multiplex IHC and final pathology. Results: The concordance rate between the pathological diagnosis made with added rapid multiplex IHC and the final pathology was 93.0% (Cohen’s 𝜅 coefficient = 0.860 and 95% CI: 0.727–0.993). When considering only adenocarcinoma and squamous cell carcinoma, the diagnoses were in agreement for all cases. Conclusions: We suggest rapid multiplex IHC as a promising tool for determining surgical strategies for lung tumors.

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Rapid intraoperative Ki-67 immunohistochemistry for lung cancer using non-contact alternating current electric field mixing

Cited by 4 publications

References 35 publications

Nomogram for the preoperative prediction of Ki-67 expression and prognosis in stage IA lung adenocarcinoma based on clinical and multi-slice spiral computed tomography features

Nomogram for the preoperative prediction of Ki-67 expression and prognosis in stage IA lung adenocarcinoma based on clinical and multi-slice spiral computed tomography features

Octreotide modified liposomes that co-deliver paclitaxel and neferine effectively inhibit ovarian cancer metastasis by specifically binding to the SSTR2 receptors

Multiplex Intraoperative Rapid Immunohistochemistry with Noncontact Antibody Mixing for Distinguishing the Histologic Phenotype of Lung Cancer

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