Rapid Inhibition of mRNA Synthesis during Preimplantation Embryo Development: Vital Permeabilization by Lysolecithin Potentiates the Action of α-Amanitin

Ma, Khidir; Jj, Stachecki; Sa, Krawetz; Armant, D. Randall

doi:10.1006/excr.1995.1272

Cited by 12 publications

(10 citation statements)

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“…At 0.5 µg/ml actinomycin D, the complete inhibition of cavitation was observed in about 85% of embryos, and the remaining embryos showed a much smaller cavity. These results, together with the previous studies (Khidir et al, 1995;Kidder and McLachlin, 1985), suggest that both compaction and cavity formation require de novo synthesis of mRNAs. It is likely that the p38 and JNK signaling pathways are involved in these processes during preimplantation development through regulation of gene expression.…”

Section: Resultssupporting

confidence: 82%

“…show that gene expression is essential for compaction and cavitation, in accordance with previous reports with another inhibitor (Khidir et al, 1995;Kidder and McLachlin, 1985). Moreover, recent microarray analyses during mouse preimplantation development revealed global gene expression changes, which could be associated with various signaling pathways (Hamatani et al, 2004;Wang et al, 2004).…”

Section: Introductionsupporting

confidence: 90%

“…Previous experiments with α-amanitin showed that gene expression is necessary for preimplantation development (Khidir et al, 1995;Kidder and McLachlin, 1985). To confirm this, we examined the influence of transcription inhibitor, actinomycin D, on preimplantation development.…”

Section: Resultsmentioning

confidence: 87%

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Requirement of the MAP kinase signaling pathways for mouse preimplantation development

et al. 2005

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Mammalian preimplantation development involves several crucial events, such as compaction and blastocyst formation, but little is known about essential genes that regulate this developmental process. Here, we have focused on MAP kinase signaling pathways as potential regulatory pathways for the process. Our results show that inhibition of the JNK pathway or of the p38 MAP kinase pathway, but not of the ERK pathway, results in inhibition of cavity formation, and that JNK and p38 are active during mouse preimplantation development. Our subsequent microarray analyses show that, of about 39,000 transcripts analyzed, the number of those genes whose expression level is sensitive to the inhibition of the JNK or the p38 pathway, but insensitive to the inhibition of the ERK pathway, is only 156. Moreover, of the 156 genes,expression of 10 genes (two genes upregulated and eight genes downregulated)is sensitive to either inhibition of the JNK or p38 pathways. These 10 genes include several genes known for their function in axis and pattern formation. Downregulation of some of the 10 genes simultaneously using siRNA leads to abnormality in cavity formation. Thus, this study has successfully narrowed down candidate genes of interest, detailed analysis of which will probably lead to elucidation of the molecular mechanism of preimplantation development.

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Section: Resultssupporting

confidence: 82%

Section: Introductionsupporting

confidence: 90%

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Requirement of the MAP kinase signaling pathways for mouse preimplantation development

et al. 2005

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“…A less specific inhibitor of transcription, actinomycin D, is also a poor inhibitor of trophoblast outgrowth (Glass et al, 1976). Conditions of α-amanitin treatment have been established that completely inhibit poly(A + ) RNA synthesis within 3 h in mouse blastocysts without affecting total RNA synthesis (Khidir et al, 1995). Culture of blastocysts with α-amanitin between GD4 and GD7 has no effect on the appearance of fibronectin-binding activity early on GD7 (Schultz et al, 1997), suggesting that genes regulating trophoblast adhesion are expressed prior to the early blastocyst stage, over 72 h before the event occurs.…”

Section: Regulation Of Gene Expressionmentioning

confidence: 99%

Blastocysts don't go it alone. Extrinsic signals fine-tune the intrinsic developmental program of trophoblast cells

Armant

2005

Developmental Biology

112

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The preimplantation embryo floats freely within the oviduct and is capable of developing into a blastocyst independently of the maternal reproductive tract. While establishment of the trophoblast lineage is dependent on expression of developmental regulatory genes, further differentiation leading to blastocyst implantation in the uterus requires external cues emanating from the microenvironment. Recent studies suggest that trophoblast differentiation requires intracellular signaling initiated by uterine-derived growth factors and integrin-binding components of the extracellular matrix. The progression of trophoblast development from the early blastocyst stage through the onset of implantation appears to be largely independent of new gene expression. Instead, extrinsic signals direct the sequential trafficking of cell surface receptors to orchestrate the developmental program that initiates blastocyst implantation. The dependence on external cues could coordinate embryonic activities with the developing uterine endometrium. Biochemical events that regulate trophoblast adhesion to fibronectin are presented to illustrate a developmental strategy employed by the peri-implantation blastocyst.

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“…TSC await initiating signals for differentiation which can be mediated by protein kinases or stress enzymes. For the trophectoderm of the implanting blastocyst, extrinsic signals initiate a posttranscriptional and posttranslational adhesion program (Armant, 2005;Khidir et al, 1995;Schultz et al, 1997). Cultured TSC can be reinjected into the blastocyst to form a blastocyst chimera.…”

Section: The "Idling Car" Hypothesismentioning

confidence: 99%

Interpreting the Stress Response of Early Mammalian Embryos and Their Stem Cells

Xie

Awonuga

Zhou

et al. 2011

International Review of Cell and Molecular Biology

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Rapid Inhibition of mRNA Synthesis during Preimplantation Embryo Development: Vital Permeabilization by Lysolecithin Potentiates the Action of α-Amanitin

Cited by 12 publications

References 0 publications

Requirement of the MAP kinase signaling pathways for mouse preimplantation development

Requirement of the MAP kinase signaling pathways for mouse preimplantation development

Blastocysts don't go it alone. Extrinsic signals fine-tune the intrinsic developmental program of trophoblast cells

Interpreting the Stress Response of Early Mammalian Embryos and Their Stem Cells

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