Rapid inhibition of Ca2+ influx by neurosteroids in murine embryonic sensory neurones

Viero, Cédric; Méchaly, Ilana; Aptel, Hervé; Puech, Sylvie; Valmier, Jean; Bancel, Frédéric; Dayanithi, Govindan

doi:10.1016/j.ceca.2006.04.007

Cited by 36 publications

(28 citation statements)

References 47 publications

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“…The membrane 25-Dx progesterone receptor, which is a putative membrane progesterone receptor (Gerdes et al, 1998;Krebs et al, 2000), may also mediate some of these effects (Sakamoto et al, 2004). This receptor is now referred to as progesterone membrane receptor component 1 (PGRMC1) (Crudden et al, 2006;Engmann et al, 2006;Peluso et al, 2006;Viero et al, 2006).…”

Section: Cerebellummentioning

confidence: 99%

Neurosteroid regulation of central nervous system development

Mellon

2007

Pharmacology & Therapeutics

186

117

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Neurosteroids are a relatively new class of neuroactive compounds, brought to prominence in the past two decades. Despite knowing of their presence in the nervous system of various species for over twenty years and knowing of their functions as GABA A and NMDA ligands, new and unexpected functions of these compounds are continuously being identified. Absence or reduced concentrations of neurosteroids during development and in adults may be associated with neurodevelopmental, psychiatric, or behavioral disorders. Treatment with physiologic or pharmacologic concentrations of these compounds may also promote neurogenesis, neuronal survival, myelination, increased memory, and reduced neurotoxicity. This review highlights what is currently known about the neurodevelopmental functions and mechanisms of action of four distinct neurosteroids -pregnenolone, progesterone, allopregnanolone and dehydroepiandrosterone.

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Section: Cerebellummentioning

confidence: 99%

Neurosteroid regulation of central nervous system development

Mellon

2007

Pharmacology & Therapeutics

186

117

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“…As described previously [28,34,35], the control and test solutions were applied using a temperature controlled multichannel polypropylene capillary perfusion system (Warner Instruments, Inc.). A single outlet capillary tubing (100 mm 1508 FOROSTYAK ET AL.…”

Section: Drug Applicationmentioning

confidence: 99%

Plasticity of Calcium Signaling Cascades in Human Embryonic Stem Cell-Derived Neural Precursors

Forostyak

Romanyuk

Verkhratsky

et al. 2013

Stem Cells and Development

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Human embryonic stem cell-derived neural precursors (hESC NPs) are considered to be a promising tool for cellbased therapy in central nervous system injuries and neurodegenerative diseases. The Ca 2 + ion is an important intracellular messenger essential for the regulation of various cellular functions. We investigated the role and physiology of Ca 2 + signaling to characterize the functional properties of CCTL14 hESC NPs during long-term maintenance in culture (in vitro). We analyzed changes in cytoplasmic Ca

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“…Only capacitative calcium entry was found to be reduced by approximately 45% in the presence of allo or DHEA (data not shown). Therefore, -and taking also into consideration the well established decrease of calcium influx in the presence of neurosteroids (Ffrench-Mullen and Spence, 1991;FfrenchMullen et al, 1994;Viero et al, 2006) -one could suggest that an elementary membrane calcium event is mediating the activation of the PI3-K/Akt pathway leading to neuronal cell survival. The above results suggest that neurosteroids may serve as endogenous neuroprotectants against acute or chronic neurotoxicity, acting in concert through several mechanisms to delay and/or protect neurons from cell death.…”

Section: Resultsmentioning

confidence: 96%

“…Also, removal of extracellular calcium can protect neuronal cells against excitotoxicity (Li J. et al, 2001). Several reports support that neurosteroids can modulate calcium influx from the plasma membrane (FfrenchMullen and Spence, 1991;1994;Viero et al, 2006) and preserve neuronal mitochondria from calcium overload (Kaasik et al, 2003), which may be an important factor responsible for their neuroprotective effects. Recently, neurosteroids have been shown to exert neuroprotective effects through activation of the phosphatidylinositol 3-kinase (PI3-K)/ Akt cascade (Zhang et al, 2002;Charalampopoulos et al, 2004).…”

Section: Introductionmentioning

confidence: 96%

Induction of Akt by endogenous neurosteroids and calcium sequestration in P19 derived neurons

Xilouri

Papazafiri

2008

neurotox res

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Neuronal cell death caused by pathophysiological over-activation of glutamate receptors and the subsequent CaII overloading, has been implicated in neurodegeneration after stroke, cerebral trauma and epileptic seizures. Recent findings suggest that certain progesterone metabolites (neurosteroids) such as allopregnanolone and dehydroepiandrosterone can protect neuronal cells from such insults. In the present study, murine P19 cells were induced to differentiate into postmitotic neurons expressing specific neuronal markers, including GABA(A) and NMDA receptors. Activation of NMDA receptors in P19-N neurons resulted in excitotoxic cell death, which involved suppression of the phosphorylation of the survival kinase PKB/Akt. Allopregnanolone and DHEA induced a rapid and prolonged phosphorylation of the Akt kinase and they were able to reverse the NMDA-induced suppression of the PI3-K/Akt pathway. The specificity of the neuroprotective effects of these neurosteroids was confirmed by the phosphatidylinositol 3-kinase (PI3-K) inhibitor wortmannin, as well as by the GABA(A) receptor antagonist, bicuculline. The neurotoxic effect of NMDA on P19-N neurons was directly correlated with increased CaII entry, since the addition of EGTA or BAPTA-AM, significantly suppressed the NMDA-induced decrease of phospho-Akt and subsequent neuronal death. These results suggest that neurosteroids are able to act as survival factors on P19-N neurons, promoting the activation of the PI3-K/Akt pathway through a calcium-entry dependent mechanism.

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Rapid inhibition of Ca2+ influx by neurosteroids in murine embryonic sensory neurones

Cited by 36 publications

References 47 publications

Neurosteroid regulation of central nervous system development

Neurosteroid regulation of central nervous system development

Plasticity of Calcium Signaling Cascades in Human Embryonic Stem Cell-Derived Neural Precursors

Induction of Akt by endogenous neurosteroids and calcium sequestration in P19 derived neurons

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