Rapid Induction of Female-to-Male Sex Change in Adult Zebrafish by Injection of an Aromatase Inhibitor

Rahaman, Md. Mostafizur; Kumagai, Ryo-Ichi; Tokumoto, Toshinobu

doi:10.1089/zeb.2020.1864

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…For example, total aromatase inhibition reduced female fathead minnow ( Pimephales promelas ; 6 ), medaka ( Oryzias latipes ; 7 ) and zebrafish ( 8 ) fecundity. These differences are likely due to the contribution of circulating estrogens produced by the ovaries via Cyp19a1a that function to maintain the integrity of the ovarian state and the reproductive capacity (i.e., fertility) of female zebrafish ( 10 ). The observed effects on female spawning behavior in the current study are linked to altered local brain estrogen production via Cyp19a1b, because brain E2 levels are significantly lower in cyp19a1b -/- mutant females compared to WT females, whilst ovarian E2 levels are similar between the groups.…”

Section: Discussionmentioning

confidence: 99%

“…In teleosts, short term chemical inhibition of aromatase reduces female fertility and fecundity through impaired oocyte development and reduces plasma vitellogenin levels (6)(7)(8). Long term aromatase inhibitor treatment induces more dramatic effects such as ovarian retraction followed by testis formation resulting in female-to-male sex change (9,10). Since chemical inhibitors are non-selective for the two Cyp19a1 isoforms, it only recently became possible to begin identifying their independent contributions to reproduction via the creation of zebrafish cyp19a1 -/mutant lines.…”

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confidence: 99%

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Mutation of brain aromatase disrupts spawning behavior and reproductive health in female zebrafish

Shaw,

Therrien,

et al. 2023

Front. Endocrinol.

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Aromatase (Cyp19a1) is the steroidogenic enzyme that converts androgens into bioactive estrogens, and hence is in a pivotal position to mediate reproduction and sexual behavior. In teleosts, there are two aromatase paralogs: cyp19a1a that is highly expressed in granulosa and Leydig cells in the gonads with critical function in sexual differentiation of the ovary, and cyp19a1b that is highly expressed in radial glial cells in the brain with unknown roles in reproduction. Cyp19a1-/- mutant zebrafish lines were used to investigate the importance of the cyp19a1 paralogs for spawning behavior and offspring survival and early development. Mutation of cyp19a1b was found to increase the latency to the first oviposition in females. Mutation of cyp19a1b in females also increased the number of eggs spawned; however, significantly more progeny died during early development resulting in no net increase in female fecundity. This finding suggests a higher metabolic cost of reproduction in cyp19a1b-/- mutant females. In males, the combined mutation of both cyp19a1 paralogs resulted in significantly lower progeny survival rates, indicating a critical function of cyp19a1 during early larval development. These data establish the specific importance of cyp19a1b for female spawning behavior and the importance of the cyp19a1 paralogs for early larval survival.

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