Rapid Induction of an Immune Response in Rat Peyer's Patch after Oral Administration of Enterococcus faecalis Strain EC-12

Inoüe, Ryo; Tsukahara, Takamitsu; Matsukawa, Noriko; Watanabe, Takumi; Bukawa, Wakoto; Nakayama, Katsuhiro; Ushida, Kazunari

doi:10.1271/bbb.120838

Cited by 10 publications

(10 citation statements)

References 13 publications

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“…1 and 4 A) was triggered in mice orally given CPEF (5 or 10 mg/kg BW/day) compared with those that were not. This result is in accordance with previous studies at these premises, where we demonstrated that a different E. faecalis strain induced an immune response in gut-associated [ 7 , 8 ] and systemic [ 9 , 10 ] lymphoid tissues. Elsewhere, researchers have also shown the immunostimulatory properties of E. faecalis strains, including activation of dendritic cells and interferon-γ production [ 37 ] and cytokine production in human peripheral blood mononuclear cells [ 38 ].…”

Section: Discussionsupporting

confidence: 94%

“…For example, it has been shown dietary LAB stimulate the NK activity of splenocytes in both young [ 4 ] and elderly mice [ 5 ], which results in suppression of tumour growth [ 6 ]. More recently, we demonstrated several times in our premises the immunostimulating effects of a cell preparation of Enterococcus faecalis (a species of LAB) not only on lymphoid tissue associated with the gut [ 7 , 8 ] but also on lymphatic systemic tissue [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Stimulation of murine cell-mediated immunity by dietary administration of a cell preparation of Enterococcus faecalis strain KH-2 and its possible activity against tumour development in mice

Tsukahara

Nakamura

Romero-Pérez

et al. 2018

Bioscience of Microbiota, Food and Health

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It is well known that dietary lactic acid bacteria (LAB) stimulate cell-mediated immunity such as natural killer (NK) activity in mice. Here, we aimed to assay the immunomodulatory effects of a cell preparation of Enterococcus faecalis strain KH-2 (CPEF). We further evaluated the possibility of antitumour activity caused by CPEF administration, because NK cells actively participate in the prevention of tumour formation. NK cell activity and gene expression of IFN-γ and Perforin 1, which were induced most likely by a synergetic action of their cytotoxic activity, were higher in splenocytes of CPEF-administered mice than they were in control mice. Moreover, unlike those of control mice, the splenocytes of CPEF-administered mice had significantly higher CD28+CD69+/CD4+ and CD28+CD69+/CD8+ ratios that resulted in a survival rate with a tendency toward improvement after 47 days of CPEF administration (p=0.1) in Meth-A fibrosarcoma-bearing mice. In conclusion, we showed that CPEF might be effective in treating Meth-A fibrosarcoma in mice, as it helped increase their survival rate via stimulation of an immune response in splenocytes, which involved systemic cellular immunity processes such as cytotoxic activity, and active T cells.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Stimulation of murine cell-mediated immunity by dietary administration of a cell preparation of Enterococcus faecalis strain KH-2 and its possible activity against tumour development in mice

Tsukahara

Nakamura

Romero-Pérez

et al. 2018

Bioscience of Microbiota, Food and Health

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“…For example, continual consumption of a milk-based drink containing a Lactobacillus brevis strain was reported to lower the incidence of influenza in unvaccinated schoolchildren in Japan ( 15 ). These findings emphasize the potential of probiotics as complement to conventional vaccine-based approaches for treatment of influenza infection ( 21 ). Nonetheless, inconsistency in the survival rate of strains ( 22 , 23 ) and lack of consensus on the effective doses ( 24 , 25 ) are issues needed to be addressed before LAB can be considered reliable as therapeutic agents against viral infections.…”

Section: Introductionmentioning

confidence: 60%

Orally Administered Salacia reticulata Extract Reduces H1N1 Influenza Clinical Symptoms in Murine Lung Tissues Putatively Due to Enhanced Natural Killer Cell Activity

et al. 2016

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Influenza is a major cause of respiratory tract infection. Although most cases do not require further hospitalization, influenza periodically causes epidemics in humans that can potentially infect and kill millions of people. To countermeasure this threat, new vaccines need to be developed annually to match emerging influenza viral strains with increased resistance to existing vaccines. Thus, there is a need for finding and developing new anti-influenza viral agents as alternatives to current treatments. Here, we tested the antiviral effects of an extract from the stems and roots of Salacia reticulata (SSRE), a plant rich in phytochemicals, such as salacinol, kotalanol, and catechins, on H1N1 influenza virus-infected mice. Following oral administration of 0.6 mg/day of SSRE, the incidence of coughing decreased in 80% of mice, and only one case of severe pulmonary inflammation was detected. Moreover, when compared with mice given Lactobacillus casei JCM1134, a strain previously shown to help increase in vitro natural killer (NK) cell activity, SSRE-administered mice showed greater and equal NK cell activity in splenocytes and pulmonary cells, respectively, at high effector cell:target cell ratios. Next, to test whether or not SSRE would exert protective effects against influenza in the absence of gut microbiota, mice were given antibiotics before being inoculated influenza virus and subsequently administered SSRE. SSRE administration induced an increase in NK cell activity in splenocytes and pulmonary cells at levels similar to those detected in mice not treated with antibiotics. Based on our results, it can be concluded that phytochemicals in the SSRE exerted protective effects against influenza infection putatively via modulation of the immune response, including enhancement of NK cell activity, although some protective effects were not necessarily through modulation of gut microbiota. Further investigation is necessary to elucidate the molecular mechanisms underlying the protective effects of SSRE against influenza infection.

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“…Key words: heat-killed cell preparation of Corynebacterium glutamicum; Shiga toxin 2e; immunoglobulin A; interleukin 12; mouse model Functional foods such as probiotics, 1,2) prebiotics 3,4) , and the cell preparations of bacteria 5,6) modulate the immune activity in the alimentary tract. Among these agents, the cell preparation of bacteria is primarily used due to its ease of handling and immunostimulating characteristics.…”

Section: And Takeshi Fujiedamentioning

confidence: 99%

Heat-killed cell preparation of Corynebacterium glutamicum stimulates the immune activity and improves survival of mice against enterohemorrhagic Escherichia coli

Ebisawa

Tsukahara

Fudou

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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Fermentation by Corynebacterium glutamicum is used by various industries to produce L-Glutamate, and the heat-killed cell preparation of this bacterium (HCCG) is a by-product of the fermentation process. In present study, we evaluated the immunostimulating and survival effects against enterohemorrhagic Escherichia coli (STEC) infection of HCCG. HCCG significantly stimulated in vitro IgA and interleukin-12 p70 production in murine Peyer's patch cells and peritoneal macrophages, respectively. Oral administration of 10 mg/kg body weight (BW) of HCCG for seven consecutive days stimulated IgA concentration in murine cecal digesta. Mice were orally administered HCCG for 17 consecutive days (d0-d17), and challenged with STEC on d4 to d6. Survival of mice tended to improve by 100 mg/kg BW of HCCG administration compared with those in control group. In conclusion, HCCG supplementation was found to prevent STEC infection in mice, and thus it may have the potential to stimulate the immune status of mammals.

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Rapid Induction of an Immune Response in Rat Peyer's Patch after Oral Administration of Enterococcus faecalis Strain EC-12

Cited by 10 publications

References 13 publications

Stimulation of murine cell-mediated immunity by dietary administration of a cell preparation of <i>Enterococcus faecalis</i> strain KH-2 and its possible activity against tumour development in mice

Stimulation of murine cell-mediated immunity by dietary administration of a cell preparation of <i>Enterococcus faecalis</i> strain KH-2 and its possible activity against tumour development in mice

Orally Administered Salacia reticulata Extract Reduces H1N1 Influenza Clinical Symptoms in Murine Lung Tissues Putatively Due to Enhanced Natural Killer Cell Activity

Heat-killed cell preparation of Corynebacterium glutamicum stimulates the immune activity and improves survival of mice against enterohemorrhagic Escherichia coli

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