Rapid in vitro Propagation of Dodonaea viscosa (Sapindaceae) Through Axillary Bud Multiplication and Indirect Organogenesis

Abstract

Protocols of axillary bud multiplication were established for Dodonaea viscosa (Sapindaceae). Murashig & Skoog’s medium with 0.2 mg/l BAP with 0.01 Naphthalene acetic acid induced high rate of shoot induction and an average of five shoots per node. Subsequent culture enhanced the number of shoots. Callus initiated from the basal cut end explants differentiated in to more than 10 shoots on MS Medium with 0.4mg/l Benzylaminopurine and 0.02mg/l Indole Butric Acid. Eighty per cent of the rooted shoots survived when transferred to greenhouse and subsequently to the field.