Rapid in-vitro plant regeneration of cotton ( Gossypium hirsutum L.)

Hemphill, John K.; Maier, Camelia; Chapman, Kent D.

doi:10.1007/s002990050391

Cited by 46 publications

(23 citation statements)

References 12 publications

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“…Delinted seeds of Coker 310FR were surface-sterilized following Hemphill et al (1998) and germinated on half-strength MS medium [MSB medium (Table 1)]. Hypocotyl (4-5 mm) and cotyledonary explants (approx.…”

Section: Methodsmentioning

confidence: 99%

Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker�310�FR)

et al. 2003

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In Agrobacterium-mediated genetic transformation of cotton (Gossypium hirsutum L. cv. Coker 310FR) the frequency at which somatic embryos were converted to plantlets was significantly improved by subjecting the embryos to slow physical desiccation. We used Agrobacterium strain GV3101 containing the binary vector pGSFR with the nos-nptII gene for in vitro selection and the 35S gus-int fragment as a reporter to optimize the transformation protocol. Although the concentration of kanamycin was reduced during embryogenesis and embryo maturation, even at the lower levels somatic embryos were predominantly abnormal, showing hypertrophy and reduced or fused cotyledons or poor radicle ends. A majority of these embryos (more than 75%) were beta-glucuronidase (GUS)-positive. Embryos with an abnormal appearance showed a very poor conversion to plantlets. However, these embryos, when subjected to slow physical desiccation followed by transfer to fresh medium, regenerated single or multiple shoots from the cotyledonary end. These shoots could be grafted on wild-type seedling stocks in vitro, which, following their transfer to soil, developed normally and set seeds. Regenerated plants tested positive for the transgene by Southern analysis. An overall scheme for the high-frequency production of cotton transgenics from both normal and abnormal appearing somatic embryos is presented.

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Section: Methodsmentioning

confidence: 99%

Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker�310�FR)

et al. 2003

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“…Theoretically, each excised apex should develop into a rooted plant; however, the yield of shoots in vitro from isolated apices depends on the incidence of contamination and rooting efficiency (Gould et al, 1991). In recent years, protocols involving proliferation of cotton shoots (Agrawal et al, 1997;Hemphill et al, 1998) have been published. The rooting efficiency ranged from 38 % to 58 % in their reports.…”

Section: Discussionmentioning

confidence: 99%

Expression of Mannose-Binding Insecticidal Lectin Gene in Transgenic Cotton (<i>Gossypium</i>) Plant

N.B.

H.M.²,

I.³

et al. 2011

cgg

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Cotton (Gossypium spp) is an important world crop. Despite the efforts made through traditional breeding methods, cotton breeders still faced with many problems, i.e., narrow genetic base, inability to use alien genes and difficulty in breaking gene linkages. Breeders attempted genetic transformations analyses tools to overcome these problems with very little success, hence the need for transgenic intervention. In this report, an optimized cotton regeneration system from shoot apices used to transform cotton wit insecticidal lectin gene from Allium sativum.Cotton regeneration system was observed to be genotype independent with a regeneration rate of 85% obtained within 16 weeks. The age of explants and the size of isolated tips have a significant effect on shoot tip elongation. The elongation rates of the three varieties were not significantly different from each other (p=0.157 3). It was observed that Samcot 9 had the highest rooting efficiency (47%), and Samcot 13 had the least rooting efficiency (36%). Though the difference in rooting efficiency was not significant in the three varieties (P=0.08). Transgenic cotton plants were obtained via Agrobacterium-mediated transformation using shoot apices as explants. Transformation rate was 1.3% using LBA 4404 with β-glucuronidase (GUS) gene. The mean number of GUS positive apices was 67% higher when acetosyringone was included in the medium. Agrobacterium concentration and co-cultivation time have a significant effect on transient GUS expression. The highest GUS positive number was observed at OD 600 0.6 and co-cultivation for 3 days. Putative transgenic plants were confirmed by leaf GUS assay, kanamycin leaf test and molecular analysis of putative young leaves.

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“…Coker 310FR) plants [43] were grown in the cotton green house at Gautam Buddha University, UP at 32˚C temperature and 16:8 hr day/night lengths. Seeds were surface sterilized using protocol of [44] and germinated on half-strength MS medium ( Table 1). The cotyledonary explants from 7-dayold seedlings were used for in vitro tissue culture at 28˚C ± 2˚C, 12:12 hr light/dark combination.…”

Section: In Vitro Tissue Culture Of Cottonmentioning

confidence: 99%

Role of Plant Somatic Embryogenesis Receptor Kinases (SERKs) in Cell-to-Embryo Transitional Activity: Key at Novel Assorted Structural Subunits

Pandey¹,

Chaudhary²

2014

AJPS

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Somatic Embryogenesis Receptor Kinase (SERK) family of receptor kinases is functionally diverse, involved in cell-to-embryo transition and controlling a number of other fundamental aspects of plant development. The morphological transformation of somatic to embryonic cells has drawn scientific attention utmost due to remarkable genetic-switch system evolved across species. Receptor kinases having direct role in somatic embryogenesis (SE) and involved in other functions are designated as "SERK" and "SERK-like" genes, respectively. We aim for phylogenetic reconstruction to reveal major SERK groups across plant species (angiosperm to gymnosperm) for their functional diversification. Data indicate that the development of SERK proteins occurred prior to the divergence of monocots and eudicots. Also, the SERK orthology is not directly proportional to their functions. Structure prediction results identified novel transmembrane topologies, short linear motifs and O-glycosylation sites exclusively in SERK proteins than SERK-like proteins. Comparative temporal expression analyses of SERK and SERK-like genes provided significant accordance with their physiological function. The identification of intrinsic disordered regions (IDRs) exclusively in SERK proteins was assumed to perceive external stress-induced signals that may lead to rapid protein folding. In a result it switches-on the precise cellular signals essential for the acquisition of SE. Moreover, the regulatory sequences of SERK genes are evolved with unique cellular fate deciding AP2-like ethylene responsive transcription factor AINTEGUMENTA binding sites for their spatial expression in SE. Based on these analyses we suggest future avenues of research that may be imperative for elucidating the importance of SERK gene evolution in SE.

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Rapid in-vitro plant regeneration of cotton ( Gossypium hirsutum L.)

Cited by 46 publications

References 12 publications

Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker�310�FR)

Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker�310�FR)

Expression of Mannose-Binding Insecticidal Lectin Gene in Transgenic Cotton (<i>Gossypium</i>) Plant

Role of Plant Somatic Embryogenesis Receptor Kinases (SERKs) in Cell-to-Embryo Transitional Activity: Key at Novel Assorted Structural Subunits

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