Rapid in Vitro Multiplication Protocol for Ceropegia Noorjahaniae Ans., a Critically Endangered, Endemic Plant of the Western Ghats

Kedage, Vinayak

doi:10.15406/apar.2017.07.00253

Cited by 1 publication

(1 citation statement)

References 5 publications

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“…Similarly maximum shoot induction and elongated shoots without basal callusing was noticed from the shoot tip explants of Woodfordia fruticosa (Asclepiadaceae) in SH medium augmented with BA and Kinetin (Krishnan and Seeni 1994). The synergic effect of BA in combination with an auxin has been demonstrated very earlier in Asclepidacean medicinal plants like Hemidesmus indicus (Sreekumar et al 2000), Ceropegia noorjahaniae (Kedage et al 2017) and Gymnema sylvestre (Veerashree and Kumar 2018). According to Balakrishnan et al (2018) shoots were initiated from nodal explants in H. ada-kodien the when MS basal medium was supplemented with 0.1 mg/lBA and 1.0 mg/l Kinetin.…”

Section: Shoot Culture Establishmentmentioning

confidence: 84%

Induction of in vitro root tubers in Holostemma annulare (Roxb.) K. Schum. for the production of bioactive metabolites

2022

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Holostemma annulare (Family Asclepiadaceae) is an invaluable vulnerable medicinal plant; the root tubers are used in Ayurveda medicine and by folk healers to treat various ailments. In this study, Schenk and Hildebrandt medium fortified with the cytokinins 6-benzyl adenine, kinetin, and auxins, including indole 3-butyric acid, indole 3-acetic acid, α-naphthaleneacetic acid, and 2,4-dichlorophenoxyacetic acid, were checked for their efficiency on root tuber induction from different explants. Adventitious root tubers were more successfully induced from in vitro leaf segments and shoots when cultured in Schenk and Hildebrandt medium supplemented with 0.5 mg/l of α -naphthaleneacetic acid. In addition, preliminary phytochemical analysis of in vitro root tubers and identification of different secondary metabolites were conducted. Thin layer chromatography and high performance thin layer chromatography analysis of the crude methanolic extracts of the in vitro root tuber identified the presence of lupeol, a bioactive triterpene. Adventitious root tuber induction offers a novel method for the in vitro production of bioactive metabolites that can be scaled up by bioreactors, thus ensuring the conservation and sustainable utilization of H. annulare. The study warrants further scale-up production and pharmacological investigation that can be extended for pharmaceutical needs.

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Section: Shoot Culture Establishmentmentioning

confidence: 84%