Rapid in vitro Clonal Propagation of Lavendula officinalis chaix a Multipurpose Plant of Industrial Importance

Chishti, Nahida; Kaloo, Zahoor A; Shawl, A. S.; Sultan, Phalisteen

doi:10.3923/pjbs.2006.514.518

Cited by 8 publications

(5 citation statements)

References 10 publications

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“…[22][23][24][25][26] Furthermore, the results achieved were in line with previous reports that showed significant achievements in terms of direct regeneration. 7,11,27 De novo plant regeneration from petiole was achieved successfully through synergistic interaction of hormones (cytokine and auxin) directly. 28,29 Additionally, formation of embryo like structures were observed but did not germinate into plantlet (Fig.…”

Section: Direct Regeneration and Multiple Shoot Induction In O Sanctummentioning

confidence: 99%

In vitro direct regeneration and Agrobacterium tumefaciens mediated in planta transformation of Ocimum sanctum L.

Khan

Husain

Rahman

2022

Preprint

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Ocimum sanctum is a multipurpose herb with highly significant medicinal properties. An in vitro direct regeneration protocol for propagation of a valuable medicinal plant Ocimum sanctum, using petiole explants has been successfully developed. The protocol employed regeneration of shoots directly, without any intervening callus using Murashige and Skoog (MS) medium fortified with 3 mg L-1 BAP + 1 mg L-1 NAA. The maximum regeneration frequency of 98% with 9.6 shoots per explants was achieved. Agrobacterium tumefaciens mediated genetic transformation (ATMT) protocol (transient and stable) was established using LBA4404 strain harboring pBI121 with uid-A reporter gene and neomycin phosphotransferase (npt-II) as selection marker. The putative transformants were screened on MS with 50 mg L-1 kanamycin and subsequently rooted on the half-strength MS medium. The confirmation was done via polymerase chain reaction (PCR) using npt-II and gus-A gene-specific primers. The maximum stable transformation frequency 70%. Thus, it is apparent that the established in vitro direct regeneration and ATMT method was suitable for integrating novel genes and modulating the metabolic flux for obtaining desired agronomic trait in planta.

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Section: Direct Regeneration and Multiple Shoot Induction In O Sanctummentioning

confidence: 99%

In vitro direct regeneration and Agrobacterium tumefaciens mediated in planta transformation of Ocimum sanctum L.

Khan

Husain

Rahman

2022

Preprint

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“…Литературатурные данные по культуре изолированных органов и тканей различных видов рода Lavandula в основном касаются вопросов клонального микроразмножения и получения оздоровленного посадочного материала [141,250,303,315,327,353,370,411,430,453,455,464,555], индукции каллусо-и морфогенеза [152,287,481,353,384,410,492,585,600,613], а также получения некоторых вторичных метаболитов -розмариновой кислоты [350,355,383,498] и других фенольных кислот [384], а также пигментов [474,580,597], биотина [596] и терпеноидов [267,358]. При этом ряд факторов, влияющих на процессы морфогенеза в изолированных культурах, затронут весьма фрагментарно и лишь у отдельных генотипов, а многие клеточные технологии у лаванды практически не разработаны (в частности, клеточная селекция, мутагенез in vitro).…”

Section: глава 2 лаванда узколистная (Lavandula Angustifolia Mill)unclassified

“…2.18 Б). В некоторых работах также показано преимущество использования ИМК для укоренения in vitro побегов разных видов лаванды [303,318,370,584]. Наряду с этим имеются сообщения о лучшем укоренении при введении в среду НУК [250,327,436], НУК и ИУК [600], или использовании безгормональной среды [288].…”

Section: рис 218 развитие адвентивных побегов на 2-м этапе микроразмножения (а) укоренение In Vitro (б) адаптация In Vivo (в) лаванды сорunclassified

“…Адаптацию in vivo проводили первые 2-3 недели при повышенной влажности воздуха (90-100%) и температуре 20-24 о С. Подобран субстрат для адаптации пробирочных растений -смесь торфа, перлита и керамзита (1:1:1). Использованные режимы адаптации к условиям in vivo обеспечивали приживаемость до 88 [125,303,327,453,555], эксплантов гипокотиля или листьев [288,600]. При этом у L. viridis и L. pedunculata было показано преимущество введения в среду БАП [315,613], у L. officinalis -БАП и ИУК [303], у L. dentate -БАП и ИМК [327], у L. vera и L. angustifoliaтидиазурона [370] или исключения гормонов из среды [353].…”

“…Использованные режимы адаптации к условиям in vivo обеспечивали приживаемость до 88 [125,303,327,453,555], эксплантов гипокотиля или листьев [288,600]. При этом у L. viridis и L. pedunculata было показано преимущество введения в среду БАП [315,613], у L. officinalis -БАП и ИУК [303], у L. dentate -БАП и ИМК [327], у L. vera и L. angustifoliaтидиазурона [370] или исключения гормонов из среды [353]. В ряде работ отмечена повышенная оводненность побегов, которую предлагали снижать с помощью изменения концентраций гормонов, солей, агара [250,370,436,613].…”

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Bionechnology of Essential Oil Plants: Creation of New Forms and Micropropagation in Vitro

Yegorova¹

2021

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This monograph presents the results of many years research devoted to the development of complex biotechnological systems of creation the new forms and clonal micropropagation of such essential oil plants as lavender (Lavandula angustifolia Mill.), sage (Salvia sclarea L.), coriander (Coriandrum sativum L.), fennel (Foeniculum vulgare Mill.), anise (Anisum vulgare Gaerth.), yarrow (Achillea spp.), essential oil rose (Rosa spp.) and geranium (Pelargonium spp.). These biotechnologies are based on the investigation of features of isolated organs, callus tissues cultivation and morphogenesis induction in vitro. Chapter I deals with the general information on the methods of cell engineering that allow creating new plant genotypes and propagating them in vitro. The following Chapters summarize biotechnological research carried out at the Research Institute of Agriculture of Crimea (the former the Institute of Essential Oil and Medicinal Plants), using aromatic plant species, mainly grown in southern Russia (lavender, coriander, sage, fennel, rose yarrow etc.). The conditions of callus cultures obtaining from different explants were optimized and peculiarities of exogenous and endogenous factors effects on the callusogenesis efficiency of studied essential oil plants were detected. The methods of plant regeneration from calluses of different passages for more than fifty cultivars and samples of lavender, sage, coriander, fennel, anise, geranium, yarrow have been developed. The peculiarities of genotype, hormonal composition of culture medium, type of explant and number of passage influence on the morphogenesis induction have been determined. The means, 6 which permit to increase the frequency of organogenesis and somatic embryogenesis of callus cultures and to regenerate the plants during 1-3 years, were revealed. Furthermore, the method for creating essential oil rose hybrids using embryo culture and clonal micropropagation in vitro was developed. The peculiarities of abiotic stress factors effect at different biotechnological objects (calluses with and without morphogenesis and zygotic embryos) have been revealed. For the first time, the selective systems in vitro for screening forms with resistance to salinity, osmotic and low temperature stress have been elaborated for lavender, sage, geranium, coriander. For lavender, geranium, yarrow, fennel, rose and sage, methods of clonal micropropagation have also been created. The influence of cultivar, medium composition, explant disposition on the shoot and number of subcultivations on the propagation efficiency was shown. Based on the analysis of the regenerants from lavender, sage, geranium, coriander, yarrow and fennel calluses, the somaclonal variability by morphology and economic valuable characteristics have been determined, and prospects for breeding forms have been revealed. One of the practical results of these scientific studies is a new cultivar of clary sage Selinzh, which was created using somaclonal variants.

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Genetic fidelity of long-term micropropagated Lavandula officinalis Chaix.: an important aromatic medicinal plant

Prasad

Shukla

Mathur

et al. 2014

Plant Cell Tiss Organ Cult

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Rapid in vitro Clonal Propagation of Lavendula officinalis chaix a Multipurpose Plant of Industrial Importance

Cited by 8 publications

References 10 publications

In vitro direct regeneration and Agrobacterium tumefaciens mediated in planta transformation of Ocimum sanctum L.

In vitro direct regeneration and Agrobacterium tumefaciens mediated in planta transformation of Ocimum sanctum L.

Bionechnology of Essential Oil Plants: Creation of New Forms and Micropropagation in Vitro

Genetic fidelity of long-term micropropagated Lavandula officinalis Chaix.: an important aromatic medicinal plant

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