Rapid immunocytochemistry for the detection of cytokeratin and vimentin: assessment of its diagnostic value in neoplastic diseases of dogs

Abstract

The rapid ICC method can be a useful tool for the diagnostic cytology of neoplastic tissues in dogs.

Rapid multiple immunofluorescent staining for the simultaneous detection of cytokeratin and vimentin in the cytology of canine tumors

“…In a recent case report, pleural and peritoneal fluid cytology preparations were examined using immunofluorescence, for which carcinoma could be diagnosed. 7 We recently developed an enzymebased rapid ICC method and validated its utility in clinical cases, 8,9 and although this rapid method may be a practical and useful tool, some limitations remain, including nonspecific background staining and/or weak immunostaining. DOI: 10.1111/vcp.12598 In the present study, we developed a rapid multiple immunofluorescent (RMIF)-ICC method for cytokeratin and vimentin detection on a single cytologic preparation and evaluated the practicality of using this method in veterinary clinics compared with the enzymebased rapid ICC.…”

“…Enzyme-based rapid ICC was performed according to previous reports. 8,9 Findings of the RMIF method were compared to an enzyme-based rapid ICC method. In addition, we attempted to sequentially apply a Giemsa stain (Merck KGaA, Darmstadt, Germany) followed by RMIF staining on the same smear.…”

“…In a recent case report, pleural and peritoneal fluid cytology preparations were examined using immunofluorescence, for which carcinoma could be diagnosed . We recently developed an enzyme‐based rapid ICC method and validated its utility in clinical cases, and although this rapid method may be a practical and useful tool, some limitations remain, including nonspecific background staining and/or weak immunostaining.…”

“…Images were captured and processed using imaging software (cellSens Standard; Olympus). Enzyme‐based rapid ICC was performed according to previous reports . Findings of the RMIF method were compared to an enzyme‐based rapid ICC method.…”

Immunohistochemical staining patterns of alpha-keratins in normal tissues from two reptile species: implications for characterization of squamous cell carcinomas

“…Epithelial tumours, including metastases, most widely retain the keratin patterns of their (normal) epithelial origin; thus, the determination of the keratin patterns of tumours is essential for cell and tumour typing [ 1 ]. Immunohistochemistry is a valuable tool for the detection of cytokeratins in normal tissues from mammals [ 2 – 4 ] and also for the accurate diagnosis of numerous epithelial tumours in mammals [ 5 – 8 ] and birds [ 9 , 10 ].…”