Rapid immobilization of viable Bacillus pseudomycoides in polyvinyl alcohol/glutaraldehyde hydrogel for biological treatment of municipal wastewater

Mehrotra, Tithi; Zaman, Mohammad Nawaid; Prasad, Bhim Bali; Shukla, Anuradha; Aggarwal, Srijan; Singh, Rachana

doi:10.1007/s11356-019-07296-z

Cited by 40 publications

(13 citation statements)

References 44 publications

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“…Repeated utilization of the immobilised bacteria preserved 100% of their metabolic activity for 10 working cycles (60 days). Some researchers state that Bacillus pseudomycoides immobilized PVA)/GA hydrogel at mass ratio of 0.03 and acidic pH of 2 maintaining cell accessibility to external environment for bioremediation of wastewater, [56] however it won't work for most of low pH non-resistant bacteria strains. Recent evaluation of patent applications revealed most efficient series of bioreactor design (magnetic fluidised bed reactors, fluidised bed bioreactor employing biofilm carriers, reverse fluidised loop reactor, fluidised bed reactor, packed-bed reactor, biological permeable barrier for removal groundwater contaminations, and layered packed bed bioreactor) [47].…”

Section: Application Of Immobilised Bacteriamentioning

confidence: 99%

“…Although cryogels have been in development since the mid-1980s, it is only within the last few years that the potential of macroporous cryogels for cell immobilisation in cell culture applications and in bioremediation approaches has been realised [19,34,35,38,[56][57][58][59][60]. Lysozymeimprinted bacterial cellulose (Lyz-MIP/BC) nanofibers were developed to recognise lysozyme via metal ion coordination.…”

Section: Application Of Immobilised Bacteriamentioning

confidence: 99%

“…The inherent characteristics of macroporous cryogels, such as their high flow permeability, low flow resistance, and minimal toxicity [60], make them suitable for applications in a range of processes including aerobic and anaerobic bioreactors [28,[61][62][63][64], the separation of cells and bio-particles [27,56,[65][66][67], scaffolds for tissue engineering and biomedical applications [35,59,68], and for the perfusion of blood products [69][70][71].…”

Section: Application Of Immobilised Bacteriamentioning

confidence: 99%

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Polymeric Materials Used for Immobilisation of Bacteria for the Bioremediation of Contaminants in Water

2021

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Bioremediation is a key process for reclaiming polluted soil and water by the use of biological agents. A commonly used approach aims to neutralise or remove harmful pollutants from contaminated areas using live microorganisms. Generally, immobilised microorganisms rather than planktonic cells have been used in bioremediation methods. Activated carbon, inorganic minerals (clays, metal oxides, zeolites), and agricultural waste products are acceptable substrates for the immobilisation of bacteria, although there are limitations with biomass loading and the issue with leaching of bacteria during the process. Various synthetic and natural polymers with different functional groups have been used successfully for the efficient immobilisation of microorganisms and cells. Promise has been shown using macroporous materials including cryogels with entrapped bacteria or cells in applications for water treatment and biotechnology. A cryogel is a macroporous polymeric gel formed at sub-zero temperatures through a process known as cryogelation. Macroporous hydrogels have been used to make scaffolds or supports for immobilising bacterial, viral, and other cells. The production of composite materials with immobilised cells possessing suitable mechanical and chemical stability, porosity, elasticity, and biocompatibility suggests that these materials are potential candidates for a range of applications within applied microbiology, biotechnology, and research. This review evaluates applications of macroporous cryogels as tools for the bioremediation of contaminants in wastewater.

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Section: Application Of Immobilised Bacteriamentioning

confidence: 99%

Section: Application Of Immobilised Bacteriamentioning

confidence: 99%

Section: Application Of Immobilised Bacteriamentioning

confidence: 99%

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Polymeric Materials Used for Immobilisation of Bacteria for the Bioremediation of Contaminants in Water

2021

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“…Immobilization also allows the convenient separation of catalyst from the reaction medium and subsequently the continuous refining and purification of the reaction products . While enzymes are generally immobilized by covalent binding to a solid support, whole cells can be encapsulated in natural polymers or synthetic hydrogels including alginate, polyurethane, polyacrylamide, and polyvinyl alcohol. , Lentikats technology for encapsulation of cells is based on controlled drying of the polyvinyl alcohol–poly(ethylene glycol) (PEG) hydrogel matrix and chemical stabilization into lens-shaped particles. Lentikats particles offer improved mechanical properties and reduced diffusional limitations compared to spherical alginate beads.…”

Section: Introductionmentioning

confidence: 99%

“…While enzymes are generally immobilized by covalent binding to a solid support, whole cells can be encapsulated in natural polymers or synthetic hydrogels including alginate, polyurethane, polyacrylamide, and polyvinyl alcohol. , Lentikats technology for encapsulation of cells is based on controlled drying of the polyvinyl alcohol–poly(ethylene glycol) (PEG) hydrogel matrix and chemical stabilization into lens-shaped particles. Lentikats particles offer improved mechanical properties and reduced diffusional limitations compared to spherical alginate beads. Several bacterial or yeast whole cells have been encapsulated in Lentikats hydrogels, with applications in biocatalysis, wastewater treatment, and microbial alcohol production. , Optimized processes are also available for the large scale printing of Lentikats beads, enabling rapid immobilization of the required biocatalyst for industrial applications.…”

Section: Introductionmentioning

confidence: 99%

Process Development for 6-Aminopenicillanic Acid Production Using Lentikats-Encapsulated Escherichia coli Cells Expressing Penicillin V Acylase

et al. 2020

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Penicillin V acylase (PVA, EC 3.5.1.11) hydrolyzes the side chain of phenoxymethylpenicillin (Pen V) and finds application in the manufacture of the pharmaceutical intermediate 6-aminopenicillanic acid (6-APA). Here, we report the scale-up of cultivation of Escherichia coli whole cells expressing a highly active PVA from Pectobacterium atrosepticum and their encapsulation in polyvinyl alcohol–poly(ethylene glycol) Lentikats hydrogels. A biocatalytic process for the hydrolysis of 2% (w/v) Pen V was set up in a 2 L reactor using the Lentikats-immobilized whole cells, with a customized setup to enable continuous downstream processing of the reaction products. The biocatalytic reaction afforded complete conversion of Pen V for 10 reaction cycles, with an overall 90% conversion up to 50 cycles. The bioprocess was further scaled up to the pilot-scale at 10 L, enabling complete conversion of Pen V to 6-APA for 10 cycles. The 6-APA and phenoxy acetic acid products were recovered from downstream processing with isolated yields of 85–90 and 87–92%, respectively. Immobilization in Lentikats beads improved the stability of the whole cells on storage, maintaining 90–100% activity and similar conversion efficiency after 3 months at 4 °C. The robust PVA biocatalyst can be employed in a continuous process to provide a sustainable route for bulk 6-APA production from Pen V.

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Tuning the Physically Induced Crystallinity of Microfabricated Bioresorbable Guides for Insertion of Flexible Neural Implants

Mousavi,

Schoutens,

Merhie

et al. 2024

Adv Materials Inter

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Devices that safely interface with the brain are critical to advancing neuroengineering. Thin and flexible neural implants show great promise alongside established silicon technologies. They therefore require a physical stiffener to allow their insertion into brain tissue. Bioresorbable polymer shanks are novel transient guides enabling accurate implantation using biocompatible materials that will be absorbed by the body over time. The development of materials with optimized stiffness and degradation is needed to provide minimally invasive probes with precise insertion capability under surgical conditions. A microfabrication protocol for the patterning of polyvinyl alcohol and its physical cross‐linking is presented, resulting in insertion guides with precise shapes and tunable degradation and stiffness. The results demonstrate a remarkable improvement in batch fabricating micro‐scale neural shanks with designed crystallinity. It results in their prolonged degradation time, evaluated in agarose gel, and remarkably improved penetrability due to the increase in mechanical stiffness. In vitro and in vivo studies support the high acceptability of this combination in interfacing with neural cells and tissue. This work represents a novel approach to the material and process engineering of bioresorbable polymers for developing fully organic and safe implants.

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Rapid immobilization of viable Bacillus pseudomycoides in polyvinyl alcohol/glutaraldehyde hydrogel for biological treatment of municipal wastewater

Cited by 40 publications

References 44 publications

Polymeric Materials Used for Immobilisation of Bacteria for the Bioremediation of Contaminants in Water

Polymeric Materials Used for Immobilisation of Bacteria for the Bioremediation of Contaminants in Water

Process Development for 6-Aminopenicillanic Acid Production Using Lentikats-Encapsulated Escherichia coli Cells Expressing Penicillin V Acylase

Tuning the Physically Induced Crystallinity of Microfabricated Bioresorbable Guides for Insertion of Flexible Neural Implants

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