Rapid identification of wine yeast species based on RFLP analysis of the ribosomal internal transcribed spacer (ITS) region

Abstract

In this study, we identified a total of 33 wine yeast species and strains using the restriction patterns generated from the region spanning the internal transcribed spacers (ITS 1 and 2) and the 5.8S rRNA gene. Polymerase chain reaction (PCR) products of this rDNA region showed a high length variation for the different species. The size of the PCR products and the restriction analyses with three restriction endonucleases (HinfI, CfoI, and HaeIII) yielded a specific restriction pattern for each species with the exception of the corresponding anamorph and teleomorph states, which presented identical patterns. This method was applied to analyze the diversity of wine yeast species during spontaneous wine fermentation.

Differentiation of Brewing and Related Yeasts Based on PCR Amplification and Restriction Fragment Length Polymorphism of Ribosomal DNA

“…bayanus. This method proved to be reproducible and of use in the rapid identification of wine yeasts 4 .…”

“…In recent times, there have been a number of reports describing the application of molecular genetic tools for the differentiation and characterisation of yeasts used in industrial fermentation 2,3,4,7,8,9,11 . Data from these studies show that RFLP patterns allow recognition of individual species as well as individual strains within a species.…”

Characterisation of Hanseniaspora Isolates with Potential Aroma-enhancing Properties in Muscat Wines

“…These studies must be accompanied by other assays to assess a correct and unambiguous identification of the yeast. Molecular methods have been used to explore this yeast biodiversity, conferring a high degree of accuracy in the final identification (Kurtzman et al, 1994(Kurtzman et al, , 2011Guillamón et al, 1998;Esteve-Zarzoso et al, 1999;Arroyo-López et al, 2006).…”

Yeast biodiversity fromVitis viniferaL., subsp. sylvestris (Gmelin) Hegi to face up the oenological consequences of climate change

“…PCR products varying in length from 390 to 880 bp were digested with HinfI, HaeIII and DdeI enzymes, and the molecular mass of the restriction products obtained was compared with those described previously in the literature [10][11][12][13]. In general, we observed 18 different profiles of the ITS-5.8S rDNA region.…”

“…The approximate lengths were determined by the use of molecular weight ladder (100 bp, Roche). PCR and RFLP fragment lengths were used for identification of yeasts by comparison of the restriction bands with those available in literature [10][11][12][13]. Several groups appeared on the basis of their RFLP of the ITS region.…”