Rapid identification of Streptococcus and Enterococcus species using diffuse reflectance-absorbance Fourier transform infrared spectroscopy and artificial neural networks

Goodacre, Royston; Timmins, Éadaoin M.; Rooney, Paul J.; Rowland, Jem J.; Kell, Douglas B.

doi:10.1111/j.1574-6968.1996.tb08342.x

Cited by 170 publications

(58 citation statements)

References 14 publications

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“…FT-IR analysis was initially performed on the plate without samples to provide a reference reading for each well. The plate was loaded onto the motorised stage of a reflectance TLC accessory, 2,[16][17][18] attached to a Bruker IFS28 FT-IR spectrometer (Bruker Spectrospin Ltd., Coventry, UK). This was equipped with a mercury-cadmiumtelluride (MCT) detector, which was cooled with liquid N 2 .…”

Section: Diffuse Reflectance-absorbance Ht Ft-ir Spectroscopymentioning

confidence: 99%

“…Vibrational spectroscopic techniques (Raman and Fourier transform infrared spectroscopy) generate biochemical 'fingerprints', which when coupled to multivariate statistical analysis are demonstrated to discriminate microorganisms to subspecies levels. [1][2][3][4][5][6] Numerous advantages of using this method of discrimination over the traditional microbiological and molecular techniques are documented, and these include speed, minimal sample preparation, automation, and relative lack of expense. In addition, recent optimisation of the techniques for implementation into the clinical environment has reduced the amount of sample required, thereby reducing isolate growth and identification times.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of diffuse-reflectance absorbance and attenuated total reflectance FT-IR for the discrimination of bacteria

Winder

Goodacre

2004

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A collection of bacteria belonging to the genus Bacillus were analysed by diffuse reflectance absorbance and attenuated total reflectance Fourier transform infrared (FT-IR) spectroscopy in the mid-infrared. The diffuse reflectance absorbance method is a rapid whole organism fingerprinting method, which generates a biochemical profile of the bacteria, where samples are presented to the FT-IR spectrometer dried on a metal carrier. The attenuated total reflectance FT-IR used in conjunction with a diamond attenuated total reflectance (ATR) accessory produces a biochemical profile of the surface chemistry of bacteria directly without the need for drying, and has not previously been used in the discrimination of bacteria. Principal component, discriminant function and hierarchical cluster analysis were performed on the data to discriminate the bacteria. The differentiation of the bacteria to species level was observed in both analyses however, it was concluded that the ATR FT-IR illustrated better sub-species differentiation of the microorganisms. This may imply that the total biochemical profiling infers discrimination to species level whereas strain specific markers are present on the cell surface chemistry.

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Section: Diffuse Reflectance-absorbance Ht Ft-ir Spectroscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of diffuse-reflectance absorbance and attenuated total reflectance FT-IR for the discrimination of bacteria

Winder

Goodacre

2004

Analyst

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“…The physiological state associated with acid crash could also be differentiated ( Grube, Schuster, and Gapes, manuscript submitted ) . An alternative method for analysis of bacterial cells was developed by Goodacre et al [ 14 ] who employed the diffuse reflectance -absorbance mode. This protocol was used in the present study.…”

Section: Introductionmentioning

confidence: 99%

Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells

Schuster

Goodacre

Gapes

et al. 2001

Journal of Industrial Microbiology and Biotechnology

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Strain degeneration in solventogenic clostridia is a known problem in the technical acetone -butanol fermentation bioprocess, especially in the continuous process mode. Clostridial strain degeneration was studied by Fourier transform infrared ( FT -IR ) spectroscopy of the bacterial cells. Degenerative variant formation in two strains, Clostridium beijerinckii NCIMB 8052 and Clostridium species AA332, was detected spectroscopically. Colonies on solid media were sampled, or assayed directly in situ by IR microscopy. It has previously been shown that the distinctive acidogenic and solventogenic physiological phases of Clostridium acetobutylicum in liquid medium can be discriminated by FT -IR spectroscopy. This was confirmed here for C. beijerinckii NCIMB 8052. The proportion of degenerate cells in a mixed population in liquid medium could be quantified, as the spectral features change in different ways during the normal growth cycle of wild type organisms and degenerate variants in batch culture. This opens a new perspective for physiology -based process monitoring and control, especially of the continuous acetone -butanol fermentation.

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“…E-mail: michel.manfait@univ-reims.fr it offers several advantages. For example, the problems, mainly baseline drifts, associated with these methods and pointed out by other investigators [7,8] can be circumvented. Its use for bacterial studies is simpler since no suspension or dehydration of the sample is required.…”

Section: Introductionmentioning

confidence: 99%

ATR‐FTIR spectroscopic investigation of E. coli transconjugants β‐lactams‐resistance phenotype

et al. 1997

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Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transeonjugants TEM-1, TEM-2, TEM-3, SHV-2, SHV-3, SHV-4. A good discrimination of the susceptible strain from the transeonjugants was obtained. Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM-2 and SHV-3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to P-lactamase point-mutations.

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Rapid identification of Streptococcus and Enterococcus species using diffuse reflectance-absorbance Fourier transform infrared spectroscopy and artificial neural networks

Cited by 170 publications

References 14 publications

Comparison of diffuse-reflectance absorbance and attenuated total reflectance FT-IR for the discrimination of bacteria

Comparison of diffuse-reflectance absorbance and attenuated total reflectance FT-IR for the discrimination of bacteria

Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells

ATR‐FTIR spectroscopic investigation of E. coli transconjugants β‐lactams‐resistance phenotype

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