Rapid identification of quarantine invasive<i>Solanum elaeagnifolium</i>by real-time, isothermal recombinase polymerase amplification assay

Lei, Rong; Yan, Zhengyue; Hu, Fan; Zhu, Shuifang; Xiong, Yufen; Fan, Xiao-Ling

doi:10.1039/c7ra10781a

Cited by 7 publications

(6 citation statements)

References 27 publications

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“…Detection is similar to Taq-Man hydrolysis probes [11] except that the probe contains an internal abasic site analog, tetrahydrofuran, that is cleaved by Endonuclease IV (nfo) [12] during the course of amplification [10]. The polymerase used is strand-displacing Bsu [10], which is more resistant to chemical inhibition than Taq, giving RPA more robustness than PCR [13]. Because DNA denaturation is performed by proteins rather than heat, RPA occurs isothermally, usually 37°C -42°C, and multiple reports document improved speed for RPA relative to PCR, often with detection within 5-7 min [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…The polymerase used is strand-displacing Bsu [10], which is more resistant to chemical inhibition than Taq, giving RPA more robustness than PCR [13]. Because DNA denaturation is performed by proteins rather than heat, RPA occurs isothermally, usually 37°C -42°C, and multiple reports document improved speed for RPA relative to PCR, often with detection within 5-7 min [13][14][15]. In addition, RPA demonstrates extreme sensitivity, often detecting tens of copies of a nucleic acid target [10,[14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

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Rapid molecular detection of macrolide resistance

2019

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BackgroundEmerging antimicrobial resistance is a significant threat to human health. However, methods for rapidly diagnosing antimicrobial resistance generally require multi-day culture-based assays. Macrolide efflux gene A, mef(A), provides resistance against erythromycin and azithromycin and is known to be laterally transferred among a wide range of bacterial species.MethodsWe use Recombinase Polymerase Assay (RPA) to detect the antimicrobial resistance gene mef(A) from raw lysates without nucleic acid purification. To validate these results we performed broth dilution assays to assess antimicrobial resistance to erythromycin and ampicillin (a negative control).ResultsWe validate the detection of mef(A) in raw lysates of Streptococcus pyogenes, S. pneumoniae, S. salivarius, and Enterococcus faecium bacterial lysates within 7–10 min of assay time. We show that detection of mef(A) accurately predicts real antimicrobial resistance assessed by traditional culture methods, and that the assay is robust to high levels of spiked-in non-specific nucleic acid contaminant. The assay was unaffected by single-nucleotide polymorphisms within divergent mef(A) gene sequences, strengthening its utility as a robust diagnostic tool.ConclusionsThis finding opens the door to implementation of rapid genomic diagnostics in a clinical setting, while providing researchers a rapid, cost-effective tool to track antibiotic resistance in both pathogens and commensal strains.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid molecular detection of macrolide resistance

2019

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“…Recently, modern methods have been exploited to detect the genome through DNA or RNA fragments in weed seedlings or seeds for early detection or identification in new regions. These technologies involve (1) reverse transcription–polymerase chain reaction (RT-PCR) or PCR after transcriptase reaction (Zhang et al 2013), which is though, an expensive and time-consuming method, and (2) recombinase polymerase amplification or recombinase polymerase amplification technology, which was found to be able to identify segments of S. elaeagnifolium DNA in as little as 1 h, while remaining a significantly cheaper method than RT-PCR (Lei et al 2017). Such a method, which enables the detection of the weed through genomic screening of seeds or seedlings in the field, is an important tool for early detection of the weed in new areas and the design of effective measures for integrated management.…”

Section: Management Optionsmentioning

confidence: 99%

Biology of Invasive Plants 5. Solanum elaeagnifolium Cav.

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Moreira

Frazão

et al. 2023

Invasive plant sci. manag.

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The family Solanaceae lists about 1,400 species worldwide, of which 143 are considered weeds (Sheppard et al. 2006). The genus Solanum is the most numerous of the family Solanaceae. Solanum elaeagnifolium Cav. belongs to the clade Leptostemonum, commonly known as the “spiny solanum” clade. Solanum elaeagnifolium is native to northern Mexico and the American Southwest. A revision for the S. elaeagnifolium clade and analytic phytokeys are provided by Knapp et al. (2017). The silvery color of its leaves and their resemblance to the leaves of the olive tree (Elaeagnus) were the reasons for naming the species elaeagnifolium (Heap and Carter 1999). Nowadays, it is commonly known as silverleaf nightshade (Boyd et al. 1984). According to Krigas et al. (2021), in northern Greece S. elaeagnifolium is also called “Lernaean Hydra,” due to its intense regrowth after herbicide treatment. Solanum elaeagnifolium is known in South Africa as silverleaf bitter apple or Satansbos (Satan’s bush), indicating how harmful it is to the country (Wilson et al. 2013). In America and other parts of the world, it has received various names over the years, such as white horsenettle, bullnettle, tomatillo, meloncillo, and trompillo (Davis et al. 1945; Kwong et al. 2006). In Algeria, farmers call it echouka, which means thorn, because of the multiple spines on the stem (Adjim and Kazi Tani 2018). In South Korea, it received the name Eun-bit-kka-ma-jung, which is a combination of its silvery coloration and a common plant in the country (Hong et al. 2014).

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“…Moreover, adjuvants such as non‐ionic surfactants could be utilized to increase herbicide efficacy against noxious weeds, 48 such as Solanum elaeagnifolium . Monitoring for new invasions, mapping technologies, early detection at field scale, 49 and the soil seedbank analysis are also significant tools that allow the effective monitoring and management of silverleaf nightshade. Monitoring actions should be conducted in a broad range of land‐use types to identify the significant reservoirs of the weed for dispersal of its propagules (such as grasslands and road networks) 28 and should also include reproducible maps by using novel mapping technologies 50 .…”

Section: Integrated Management Of Important Ips: Two Case‐studiesmentioning

confidence: 99%

Early detection, herbicide resistance screening, and integrated management of invasive plant species: a review

Tataridas

Jabran

Kanatas

et al. 2022

Pest Management Science

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Invasive plant species (IPS) are often considered weeds that cause high yield losses in crops, negatively affect the environment, and disrupt certain ecosystem services. The negative impact of IPS on biodiversity is increasing and disturbing native vegetation. The management of plant invasions can be divided in two phases (before and after invasion). Prior to introduction it is crucial to develop the knowledge base (biology, ecology, distribution, impact, management) on IPS, prevention measures and risk assessment. After introduction if eradication fails, the monitoring and the integrated management of IPS are imperative to prevent the naturalization and further dispersal. This review uses two major invasive weed species (Amaranthus palmeri S. Wats. and Solanum elaeagnifolium Cav.) as case studies to propose a framework for early detection, rapid herbicide resistance screening, and integrated management. The holistic framework that is presented exploits recent: (i) novel detection tools, (ii) rapid tests and assays for herbicide resistance, and (iii) biology, ecology, distribution traits, and management tools for the IPS. Farmers, advisors, researchers, and policymakers need briefing on IPS growth dynamics, adaptability rates, and response to conventional and novel treatments to prevent new invasions, eradicate isolated stands, and mitigate the impact of invasive weed species in the long term.

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Rapid identification of quarantine invasiveSolanum elaeagnifoliumby real-time, isothermal recombinase polymerase amplification assay

Abstract: An easy-to-implement strategy to identifySolanum elaeagnifoliumby utilizing recombinase polymerase amplification (RPA) technology was developed.

Cited by 7 publications

References 27 publications

Rapid molecular detection of macrolide resistance

Rapid molecular detection of macrolide resistance

Biology of Invasive Plants 5. Solanum elaeagnifolium Cav.

Early detection, herbicide resistance screening, and integrated management of invasive plant species: a review

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