Rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by MALDI‐TOF‐MS

Stingu, Catalina‐Suzana; Rodloff, Arne C.; Jentsch, Holger; Schaumann, Reiner; Eschrich, Klaus

doi:10.1111/j.1399-302x.2008.00438.x

Cited by 76 publications

(47 citation statements)

References 22 publications

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“…A database was created, and analysis of results revealed identifications consistent with phenotypic and 16S rRNA gene sequencing data. The technology was able to differentiate between two species of Prevotella, P. intermedia and P. nigrescens, a task which would usually be relegated to costly and time-consuming 16S rRNA gene analysis (245).…”

Section: Anaerobic Bacteriamentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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SUMMARY Within the past decade, clinical microbiology laboratories experienced revolutionary changes in the way in which microorganisms are identified, moving away from slow, traditional microbial identification algorithms toward rapid molecular methods and mass spectrometry (MS). Historically, MS was clinically utilized as a high-complexity method adapted for protein-centered analysis of samples in chemistry and hematology laboratories. Today, matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) MS is adapted for use in microbiology laboratories, where it serves as a paradigm-shifting, rapid, and robust method for accurate microbial identification. Multiple instrument platforms, marketed by well-established manufacturers, are beginning to displace automated phenotypic identification instruments and in some cases genetic sequence-based identification practices. This review summarizes the current position of MALDI-TOF MS in clinical research and in diagnostic clinical microbiology laboratories and serves as a primer to examine the “nuts and bolts” of MALDI-TOF MS, highlighting research associated with sample preparation, spectral analysis, and accuracy. Currently available MALDI-TOF MS hardware and software platforms that support the use of MALDI-TOF with direct and precultured specimens and integration of the technology into the laboratory workflow are also discussed. Finally, this review closes with a prospective view of the future of MALDI-TOF MS in the clinical microbiology laboratory to accelerate diagnosis and microbial identification to improve patient care.

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Section: Anaerobic Bacteriamentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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“…Several studies have also investigated subsets of organisms, such as difficult-to-identify Nocardia spp., anaerobes, and oral flora, as well as fastidious upper respiratory flora (e.g., Neisseria spp., Moraxella spp., Haemophilus spp.) (5,9,12,13,15,16). Haemophilus spp., including H. influenzae, were previously shown to be distinguishable by MALDI-TOF MS (5), and since H. influenzae and HACEK organisms have similar growth requirements, this suggested that MALDI-TOF MS may be useful in their identification.…”

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confidence: 99%

Identification of HACEK Clinical Isolates by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2011

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Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is a rapid and accurate tool for the identification of many microorganisms. We assessed this technology for the identification of 103 Haemophilus parainfluenzae, Aggregatibacter aphrophilus, Aggregatibacter actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella kingae (HACEK) clinical isolates and 20 Haemophilus influenzae clinical isolates. Ninety-three percent of HACEK organisms were identified correctly to the genus level using the Bruker database, and 100% were identified to the genus level using a custom database that included clinical isolates.Clinical microbiology laboratories strive to identify infectious organisms from patient samples in the most-accurate yet time-and cost-efficient manner possible. This process may be impeded by rare or difficult-to-identify organisms such as those of the HACEK group (Haemophilus parainfluenzae, Aggregatibacter aphrophilus, Aggregatibacter actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella kingae). HACEK organisms are fastidious Gram-negative rods found in the healthy oropharynx or upper respiratory tract and can cause endocarditis, especially in young children and patients with heart defects (3). HACEK organisms are also involved in a wide array of serious infections involving the head and neck, bone, joints, lungs, and other soft tissues (17).Numerous reports have recently been published showing the successful integration of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in the identification of microorganisms routinely encountered in clinical microbiology labs (1,2,12,15). These reports have focused largely on easy-to-culture bacteria and yeasts, including Staphylococcus aureus and nonfermenting 14). To date, three genera of fastidious Gram-negative rods (Legionella, Bartonella, and Francisella) have been extensively evaluated by the newer MALDI-TOF MS identification systems (4, 7, 10, 11). Several studies have also investigated subsets of organisms, such as difficult-to-identify Nocardia spp., anaerobes, and oral flora, as well as fastidious upper respiratory flora (e.g., Neisseria spp., Moraxella spp., Haemophilus spp.) (5,9,12,13,15,16). Haemophilus spp., including H. influenzae, were previously shown to be distinguishable by MALDI-TOF MS (5), and since H. influenzae and HACEK organisms have similar growth requirements, this suggested that MALDI-TOF MS may be useful in their identification. HACEK organisms are not readily identified on most automated bacterial identification systems, and most require either timeconsuming biochemical profiling or genetic analysis, such as 16S rRNA gene sequencing, for definitive identification (8). This study aimed to determine whether MALDI-TOF MS could accurately identify a large panel of HACEK clinical isolates.HACEK and H. influenzae isolates were grown on chocolate agar plates (Hardy Diagnostics, Santa Maria, CA), and 24-to 48-h-old cult...

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“…Indeed, mass spectra (m/z range between 2000 and 13000) obtained for A. schaalii strains appear to be unique and reproducible. Notingly, all the databases now available do not include the spectrum profile for this species and must be updated (11,17).…”

Section: Introductionmentioning

confidence: 99%

Actinobaculum shaalii: a new uropathogen?

2015

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SummaryBackground and Aims. Actinobaculum schaalii is a facultative anaerobic, Gram-positive rod-shaped species phylogenetically related to Actinomyces. A. schaalii is an emerging pathogen causing urinary tract infections (UTI) in both children and adults; although, as part of the human genitourinary tract flora, it is frequently overlooked or considered as a contaminant. While the phenotypic identification of A. schaalii is difficult, the recent Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight-mass spectrometry (MALDI TOF) technology could represent a promising tool for its identification.Materials and Methods. This is a retrospective study including all known cases (n=7) of A. schaalii infections occurred (between July 2013 and November 2013) at the Microbiology Laboratory of the A. Cardarelli Hospital, in Campobasso (Italy).Results. All the 7 A. schaalii collected strains, resulted in vitro susceptible to most of the drugs commonly used for urinary tract infections, but resistant to ciprofloxacin, a first-line antibiotic in the treatment of prostatitis. All isolates were susceptible to amoxicillin, amoxicillin-clavulanic, ampicillin-sulbactam, cefuroxime, gentamicin, piperacillin-tazobactam, vancomicin, tetracycline (no EUCAST breakpoints). All except two isolates were susceptible to cefotaxime; 3/7 and 5/7 strains were clindamicin and levofloxacin resistant, respectively.Conclusions. As most antibiotics empirically prescribed for UTI (mainly fluoroquinolones or trimethoprim/sulfamethoxazole) are not effective against A. schaalii, the appropriate onset of treatment was delayed by an average of 2.8 days. The implementation of the newer MALDI TOF technology in routine diagnostic procedures may allow a more reliable and rapid identification of A. schaalii in future.

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Rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by MALDI‐TOF‐MS

Abstract: Our results suggest that MALDI-TOF-MS might become a useful method for the identification of anaerobic bacteria, especially for those that cannot be readily identified by biochemical analysis. It may become an attractive system even for the routine identification of clinical isolates.

Cited by 76 publications

References 22 publications

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Identification of HACEK Clinical Isolates by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Actinobaculum shaalii: a new uropathogen?

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