Rapid identification of Ochroconis gallopava by a loop-mediated isothermal amplification (LAMP) method

Ohori, Akira; Endo, Shigeo; Sano, Ayako; Yokoyama, Koji; Yarita, Kyoko; Yamaguchi, Masashi; Kamei, Katsuhiko; Miyaji, Makoto; Nishimura, Kazuko

doi:10.1016/j.vetmic.2005.11.064

Cited by 33 publications

(21 citation statements)

References 7 publications

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“…The most attractive characteristic of the LAMP method is the visual determination of nucleic acid amplification, 18 which cannot only be used in well-equipped hospitals or laboratories in developed countries, but also in small-scale hospitals or even in field detection. 37 The LAMP method has been used in the differential diagnosis of the pathogens with close genetic relationships 16,17,38,39 and even in the differentiation of DEN virus serotypes. 40 The appearance and use of a turbidimeter allowed the LAMP method to achieve real-time detection.…”

Section: Discussionmentioning

confidence: 99%

“…yeasts 16 and fungi identification. 17 According to a previous study, the sensitivity of the LAMP method is below 10 copies/ reaction. 18 Because of the high amplification efficiency, the LAMP reaction can be ended within 1 hour under isothermal conditions (60-65 C) and sophisticated instruments are not required, 18 which makes this method adaptive to field diagnosis.…”

Section: Introductionmentioning

confidence: 99%

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Rapid Identification of Chikungunya and Dengue Virus by a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Method

Lü

Li²,

Mo³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Both Chikungunya and Dengue virus belong to the acute arthropod-borne viruses. Because of the lack of specific symptoms, it is difficult to distinguish the two infections based on clinical manifestations. To identify and quantitatively detect Chikungunya and Dengue viruses, a real-time accelerated reverse-transcription-loop-mediated isothermal amplification (RT-LAMP) platform was developed, and 26-confirmed RNA samples, 42 suspects, and 18 healthy serum samples were evaluated by the method. The RT-polymerase chain reaction (PCR) and cDNA sequencing were used as references. The results showed that it could identify the Chikungunya and Dengue virus RNA correctly in all antibody-positive samples within 1 hour, without any cross-reactions. The virus load of the positive samples was quantitatively detected with a turbidimeter. The sensitivity was 100% and specificity was 95.25%. The findings indicate that the RT-LAMP is an effective method for rapid quantity detection of Chikungunya virus and Dengue virus in serum samples with convenient operation, high specificity, and high sensitivity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid Identification of Chikungunya and Dengue Virus by a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Method

Lü

Li²,

Mo³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…The LAMP primers developed in this investigation targeted conserved sequences inside the fungal D1/D2 domains of the 26S rDNA. This rDNA region was chosen not only because it is much used in yeast systematics and identification studies but also because of the availability of its sequences in public databases and its earlier utilization for designing fungal LAMP primers (34). Unfortunately, some of the available sequences were of low quality and/or were incorrectly labeled.…”

Section: Vol 46 2008 Identification Of Clinically Relevant Yeast Spmentioning

confidence: 99%

“…There has been a clear emphasis on the diagnosis of viral and bacterial infections (8,11,13,16,17,35,37,42), but parasites such as Plasmodium falciparum (36) and Trypanosoma spp. (19,44) and pathogenic fungi such as Paracoccidioides brasiliensis (7) and Ochroconis gallopava (34) have also been addressed. LAMP-based approaches have been applied to a wide range of samples, such as paraffin-embedded tissues (7), whole blood (36), nasopharyn-geal swabs (17,40), dental plaques (23), eggs (13), and potato leaf samples (32).…”

mentioning

confidence: 99%

Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

2008

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The occurrence of invasive mycoses has progressively increased in recent years. Yeasts of the genus Candida remain the leading etiologic agents of those infections. Early identification of opportunistic yeasts may contribute significantly to improved disease management and the selection of appropriate antifungal therapy. We developed a rapid and reliable molecular identification system for clinically relevant yeasts that makes use of nonspecific primers to amplify a region of the 26S rRNA gene, followed by reverse hybridization of the digoxigenin-labeled products to a panel of species-specific oligonucleotide probes arranged on a nylon membrane macroarray format. DNA amplification was achieved by the recently developed loop-mediated isothermal DNA amplification technology, a promising option for the development of improved laboratory diagnostic kits. The newly developed method was successful in distinguishing among the major clinically relevant yeasts associated with bloodstream infections by using simple, rapid, and cost-effective procedures and equipment.

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“…Each aliquot of 200 µL of swab specimens was tested by the LAMP method, as described above. On the other hand, aliquots of 20 methods are available Endo et al, 2004;Kawano et al, 2015;Ohori et al, 2006;Uemura et al, 2008;Yo et al, 2016 . In this study, we developed a LAMP method using a primer set for detecting a wide range of fungi from environmental specimens to evaluate the fungal burden, which should help to prevent allergies or infections caused by fungi.…”

Section: Detection Of Fungi In the Indoor Environmentmentioning

confidence: 99%

Detection of Fungi from an Indoor Environment using Loop-mediated Isothermal Amplification (LAMP) Method

Nakayama¹,

Yamazaki²,

Yo³

et al. 2017

Biocontrol Sci.

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Loop-mediated isothermal amplification LAMP is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans Ascomycota , Cryptococcus neoformans Basidiomycota , and Mucor racemosus Mucorales . The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction. In this study, we evaluated the correlation between colony forming units CFU and LAMP detection rate using the LAMP method for environmental fungi. The LAMP method should be a useful means of detecting fungi in indoor environments, disaster areas, or even in confined manned spacecraft to prevent allergies or infections caused by fungi.

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Rapid identification of Ochroconis gallopava by a loop-mediated isothermal amplification (LAMP) method

Cited by 33 publications

References 7 publications

Rapid Identification of Chikungunya and Dengue Virus by a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Method

Rapid Identification of Chikungunya and Dengue Virus by a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Method

Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

Detection of Fungi from an Indoor Environment using Loop-mediated Isothermal Amplification (LAMP) Method

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