“…By calculating the weighted sum of the PLS regression coefficients, we identified significant buckets with the most outstanding contribution to cluster segregation in PLS-DA, and assigned their metabolites ( Moreno-Barea et al, 2022 ). The RF technique combines many decision trees constructed by classifying each tree and voting for popular classes by bootstrap sampling ( Percival et al, 2021 ). The number of trees selected was 5000 ( Gandhi et al, 2022 ).…”