Rapid identification of <i>Streptomyces</i> tetracycline producers by MALDI-TOF mass spectrometry

Hleba, Lukáš; Charousová, Ivana; Císarová, Miroslava; Kováčik, Anton; Kormanec, Ján; Medo, Juraj; Božík, Matěj; Javoreková, Soňa

doi:10.1080/10934529.2018.1474581

Cited by 12 publications

(9 citation statements)

References 40 publications

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“…These tetracycline-producing streptomyces present important signal and protein spectra in the mass range of 2 to 20 kDa. The mass spectrum of tetracycline (dissolved in ethanol) (M + H +) was found at 445.41 m/z [39]. This study can be an anchor point for our study, since the detection of specific peaks for tetracycline in our work did not achieve final results when compared to other antibiotics.…”

Section: Tetracyclinementioning

confidence: 71%

Putative Protein Biomarkers of Escherichia coli Antibiotic Multiresistance Identified by MALDI Mass Spectrometry

Sousa

Viala

Théron

et al. 2020

Biology

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The commensal bacteria Escherichia coli causes several intestinal and extra-intestinal diseases, since it has virulence factors that interfere in important cellular processes. These bacteria also have a great capacity to spread the resistance genes, sometimes to phylogenetically distant bacteria, which poses an additional threat to public health worldwide. Here, we aimed to use the analytical potential of MALDI-TOF mass spectrometry (MS) to characterize E. coli isolates and identify proteins associated closely with antibiotic resistance. Thirty strains of extended-spectrum beta-lactamase producing E. coli were sampled from various animals. The phenotypes of antibiotic resistance were determined according to Clinical and Laboratory Standards Institute (CLSI) methods, and they showed that all bacterial isolates were multi-resistant to trimethoprim-sulfamethoxazole, tetracycline, and ampicillin. To identify peptides characteristic of resistance to particular antibiotics, each strain was grown in the presence or absence of the different antibiotics, and then proteins were extracted from the cells. The protein fingerprints of the samples were determined by MALDI-TOF MS in linear mode over a mass range of 2 to 20 kDa. The spectra obtained were compared by using the ClinProTools bioinformatics software, using three machine learning classification algorithms. A putative species biomarker was also detected at a peak m/z of 4528.00.

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Section: Tetracyclinementioning

confidence: 71%

Putative Protein Biomarkers of Escherichia coli Antibiotic Multiresistance Identified by MALDI Mass Spectrometry

Sousa

Viala

Théron

et al. 2020

Biology

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“…Polyketides exhibit a wide range of bioactivities such as antibacterial (e.g., tetracycline), antifungal (e.g., amphotericin B), anticancer (e.g., doxorubicin), antiviral (e.g., balticolid), immune-suppressing (e.g., rapamycin), anti-cholesterol (e.g., lovastatin), and anti-inflammatory activity (e.g., flavonoids) [3,4,5,6,7,8,9]. Some organisms can produce polyketides such as bacteria (e.g., tetracycline from Streptomyces aureofaciens ) [10], fungi (e.g., lovastatin from Phomopsis vexans ) [11], plants (e.g., emodin from Rheum palmatum ) [12], protists (e.g., maitotoxin-1 from Gambierdiscus australes ) [13], insects (e.g., stegobinone from Stegobium paniceum ) [14], and mollusks (e.g., elysione from Elysia viridis ) [15]. These organisms could use the polyketides they produce as protective compounds and for pheromonal communication in the case for insects.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Polyketides in Streptomyces

2019

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Polyketides are a large group of secondary metabolites that have notable variety in their structure and function. Polyketides exhibit a wide range of bioactivities such as antibacterial, antifungal, anticancer, antiviral, immune-suppressing, anti-cholesterol, and anti-inflammatory activity. Naturally, they are found in bacteria, fungi, plants, protists, insects, mollusks, and sponges. Streptomyces is a genus of Gram-positive bacteria that has a filamentous form like fungi. This genus is best known as one of the polyketides producers. Some examples of polyketides produced by Streptomyces are rapamycin, oleandomycin, actinorhodin, daunorubicin, and caprazamycin. Biosynthesis of polyketides involves a group of enzyme activities called polyketide synthases (PKSs). There are three types of PKSs (type I, type II, and type III) in Streptomyces responsible for producing polyketides. This paper focuses on the biosynthesis of polyketides in Streptomyces with three structurally-different types of PKSs.

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“…Klebsiella pneumoniae local database was created by the same protocol as in our previous study [ 29 ]. Reproducibility was verified using a blind test where random isolates of non-KPC and KPC K. pneumoniae were chosen and re-analyzed.…”

Section: Methodsmentioning

confidence: 99%

“…Genetic analysis including cell lysis and DNA extraction is time consuming, requires experienced technicians, and is also expensive [ 22 , 25 , 26 ]. In contrast, MALDI-TOF is easier, faster, cheaper, more accurate, and allows for the identification of closely related microbial strains [ 27 , 28 , 29 , 30 , 31 ]. Moreover, the analysis of unique protein biomarkers in the obtained mass spectrum allows discrimination of bacteria at the strain level [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Carbapenemase Producing Klebsiella pneumoniae (KPC): What Is the Best MALDI-TOF MS Detection Method

et al. 2021

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Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria is a group of highly dangerous antibiotic resistant Gram-negative Enterobacteriaceae. They cause infections associated with significant morbidity and mortality. Therefore, the rapid detection of KPC-producing bacteria plays a key role in clinical microbiology. Matrix assisted laser desorption/ionization time-of- flight (MALDI-TOF) is a rapidly evolving technology that finds application in various clinical, scientific, and industrial disciplines. In the present study, we demonstrated three different procedures of carbapenemase-producing K. pneumoniae (KPC) detection. The most basic model of MALDI-TOF instrument MS Microflex LT was used, operating in the linear ion-positive mode, commonly used in modern clinical laboratories. The first procedure was based on indirect monitoring of carbapenemase production with direct detection of hydrolyzed carbapenem antibiotic degradation products in the mass spectrum. The second procedure was based on direct detection of blaKPC accompanying peak with an 11,109 Da in the mass spectrum of carbapenemase-producing K. pneumoniae (KPC), which represents the cleaved protein (pKpQIL_p019) expressed by pKpQIL plasmid. In addition, several unique peaks were detected in the carbapenemase-producing K. pneumoniae (KPC) mass spectrum. The third procedure was the identification of carbapenemase-producing K. pneumoniae (KPC) based on the protein fingerprint using local database created from the whole mass spectra. By comparing detection procedures, we determined that the third procedure was very fast and relatively easy. However, it requires previous verification of carbapenemase-producing K. pneumoniae (KPC) using other methods as genetic blaKPC identification, detection of carbapenem degradation products, and accompanying peak with 11,109 Da, which represents cleaved pKpQIL_p019 protein expressed by pKpQIL plasmid. Detection of carbapenemase-producing K. pneumoniae using MALDI-TOF provides fast and accurate results that may help to reduce morbidity and mortality in hospital setting when applied in diagnostic situations.

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Rapid identification of Streptomyces tetracycline producers by MALDI-TOF mass spectrometry

Cited by 12 publications

References 40 publications

Putative Protein Biomarkers of Escherichia coli Antibiotic Multiresistance Identified by MALDI Mass Spectrometry

Putative Protein Biomarkers of Escherichia coli Antibiotic Multiresistance Identified by MALDI Mass Spectrometry

Biosynthesis of Polyketides in Streptomyces

Carbapenemase Producing Klebsiella pneumoniae (KPC): What Is the Best MALDI-TOF MS Detection Method

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