Rapid Identification of Colonization Factor Antigens (CFA/I and CFA/II) of Enterotoxigenic E. coli (ETEC) and O-Antigens of Enteropathogenic E. coli (EPEC) by Coagglutination Test

Faris, Ahmed; Ljungh, Åsa; Wadström, Torkel

doi:10.1016/s0176-6724(85)80079-1

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…HA is due to hydrophobic surface fimbriae (6). Since the C. pylon strains in this study caused MRHA but expressed a hydrophilic cell surface, hydrophobic surface fimbriae or fibrillae are not likely to cause HA in C. pylon.…”

Section: Discussionmentioning

confidence: 68%

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Mannose-resistant Haemagglutination by Campylobacter pylori

Emödy

Carlsson

Ljungh

et al. 1988

Scandinavian Journal of Infectious Diseases

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10 Campylobacter pylori strains were screened for the presence of presumptive colonization factors by measuring cell surface hydrophobicity and haemagglutination. All 8 clinical isolates and 2 reference strains caused haemagglutination of rabbit, human and/or sheep erythrocytes in the presence of mannose. None of the strains expressed a hydrophobic cell surface. In preliminary experiments the haemagglutination could be inhibited with monosialogangliosides suggesting that these strains express sialic acid specific haemagglutinin(s) which could be involved in colonization of mucosal surfaces.

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Section: Discussionmentioning

confidence: 68%

“…Cell surface hydrophobicity was measured by the salt aggregation test, SAT (6). Cell surface hydrophobicity was measured by the salt aggregation test, SAT (6).…”

Section: Methodsmentioning

confidence: 99%

Mannose-resistant Haemagglutination by Campylobacter pylori

Emödy

Carlsson

Ljungh

et al. 1988

Scandinavian Journal of Infectious Diseases

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“…The methodology has been described elsewhere (Faris et al, 1984). Briefly, 1 ml of a 10% (w/v) suspension of heat-killed and formalin-treated Staphylococcus aureus (strain Cowan I; A.T.T.C.…”

Section: Preparation Of Coagglutination Reagentmentioning

confidence: 99%

Purification and characterization of CS2, a sialic acid-specific haemagglutinin of enterotoxigenic Escherichia coli

Sjöberg

Lindahl

Porath

et al. 1988

Biochemical Journal

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CS2 fimbriae of enterotoxigenic Escherichia coli were purified and characterized. The surface haemagglutinins (fimbriae) were detached by sonication from a strain producing only the CS2 fimbriae. Isolation was carried out by gel filtration on a Sepharose 4B column. After depolymerization, the fimbriae subunits were purified on a Sephacryl S-300 column in 8.0 M-guanidinium chloride. From 1 litre of medium, 4-6 mg of purified fimbriae was obtained. We found that CS2 fimbriae were completely dissociated by saturated guanidinium chloride into subunits with a molecular mass of 16.5 kDa. CS2 fimbriae was sialic acid-specific, since sialic acids were the most potent inhibitors, and neuraminidase treatment of erythrocytes abolished haemagglutination. Both fimbriae and fimbrial subunits were found to bind to bovine erythrocytes. The binding of subunits to erythrocytes could be inhibited with low concentrations of sialyl-lactose.

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Experimental haematogenous pyelonephritis in mice with uropathogenic, enteropathogenic and enterotoxigenic

Sharma

Asnani

Banerjee

et al. 1986

Antonie van Leeuwenhoek

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Acute haematogenous pyelonephritis was produced with known uropathogenic (075), enteropathogenic (0111) and enterotoxigenic (H10407) Escherichia coli in mice. The enterotoxigenic E. coli showed the highest bacterial counts in kidney and urine and was found to have the same potential for causing pyelonephritis as a known pyelonephritogenic strain both in untreated and corticosteroid treated mice. Enteropathogenic E. coli was found to be less pyelonephritogenic in untreated mice, but under the effect of corticosteroids it showed similar pyelonephritogenic potential.

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Rapid Identification of Colonization Factor Antigens (CFA/I and CFA/II) of Enterotoxigenic E. coli (ETEC) and O-Antigens of Enteropathogenic E. coli (EPEC) by Coagglutination Test

Cited by 4 publications

References 10 publications

Mannose-resistant Haemagglutination by Campylobacter pylori

Mannose-resistant Haemagglutination by Campylobacter pylori

Purification and characterization of CS2, a sialic acid-specific haemagglutinin of enterotoxigenic Escherichia coli

Experimental haematogenous pyelonephritis in mice with uropathogenic, enteropathogenic and enterotoxigenic

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