Rapid identification of Candida albicans and its related species Candida stellatoidea and Candida dubliniensis by a single PCR amplification using primers specific for the repetitive sequence (RPS) of Candida albicans

Kanbe, Toshio; Kurimoto, Keisuke; Hattori, Hisao; Iwata, Takako; Kikuchi, Akihiko

doi:10.1016/j.jdermsci.2005.06.007

Cited by 13 publications

(14 citation statements)

References 18 publications

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“…In addition, amplification profiles of C. dubliniensis were quite different from those of C. albicans. This suggested that PCR using P-II was useful not only for genotyping of C. albicans but also for discrimination between C. albicans and C. dubliniensis [19].…”

Section: Discussionmentioning

confidence: 99%

“…The numbers of ALT lead to variations in molecular sizes of RPSs [17], and these molecular characteristics of the different sizes and copy numbers of ALT sequences are attractive targets for genotyping of C. albicans [18]. Recently, we found that C. stellatoidea and C. dubliniensis also contained RPS sequences similar to those of C. albicans, and RPSs of C. albicans were more variable compared with those of C. stellatoidea and C. dubliniensis [19]. In this study, we designed primers specific to RPS sequences of C. albicans, and PCR amplification using these primers was tested for its performance in terms of resolution, reproducibility and specificity for strain identification of C. albicans.…”

Section: Introductionmentioning

confidence: 99%

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Genotyping of Candida albicans on the basis of polymorphisms of ALT repeats in the repetitive sequence (RPS)

Iwata

Hattori

Chibana

et al. 2006

Journal of Dermatological Science

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genotyping of Candida albicans on the basis of polymorphisms of ALT repeats in the repetitive sequence (RPS)

Iwata

Hattori

Chibana

et al. 2006

Journal of Dermatological Science

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“…For genotyping of C. albicans strains, primer sets for 25S rDNA (CA-INT-L/CA-INT-R) and the RPS (ASDcF/pCSCR) were used [17]. The primer sets CA-INT-L/CA-INT-R and ASDcF/pCSCR were designated P-I and P-II, respectively [22,23]. The nucleotide sequences of the primers and the expected PCR product sizes are listed in Table 2.…”

Section: Pcr Primersmentioning

confidence: 99%

“…The numbers of ALT repeats in the RPS vary in each chromosome, thereby leading to variation in the molecular sizes of RPSs, and these molecular characteristics of the different sizes and copy numbers of the ALT sequence are attractive for the genotyping of C. albicans. Recently, we reported that a PCR system targeting the RPS region containing the inner ALT repeat sequences was quite powerful for distinguishing C. albicans from its related species C. stellatoidea and C. dubliniensis [22]. Furthermore, we described that a combined PCR system targeting 25S rDNA and RPS produced a high performance as a tool for C. albicans genotyping [23].…”

Section: Introductionmentioning

confidence: 99%

Genotype analysis of Candida albicans isolates obtained from different body locations of patients with superficial candidiasis using PCRs targeting 25S rDNA and ALT repeat sequences of the RPS

Hattori

Iwata

Nakagawa

et al. 2006

Journal of Dermatological Science

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“…Therefore, Candida albicans and non-albicans were identified by using PCR-RFLP, which is a rapid and cost-effective molecular method [18, 19]. …”

Section: Introductionmentioning

confidence: 99%

Identification of Candida species in the oral cavity of diabetic patients

Dehghan¹,

Mohammadi²,

Javaheri³

et al. 2016

mazu-cmm

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Background and Purpose:Diabetic patients are more susceptible to oral candidiasis infection than non-diabetics due to the factors promoting oral carriage of Candida. Several factors can increase colonization of Candida species in the oral cavity such as xerostomia, which reduces the salivary flow and is a salivary pH disorder. In the current study, we aimed to identify and compare the colonization level of Candida spp. in the oral cavity of diabetic and non-diabetic groups. Materials and Methods:Swabs were taken from the mouth of 106 participants and were cultured on Sabouraud dextrose agar (SDA) medium. Likewise, the saliva samples were collected for salivary glucose and pH measurements. The study was performed during June 2014-September 2015 on two groups of diabetic patients (n=58) and non-diabetics (n=48) as the control group. The Candida spp. were identified with PCR-restriction fragment length polymorphism (RFLP) using the restriction enzymes HinfI and MspI and were differentiated by culture on CHROMagar Candida medium. Results:The frequency of Candida spp. was higher in diabetic patients compared to non-diabetics. The most frequent Candida spp. in the diabetic patients were Candida albicans (%36.2), C. Krusei (%10.4), C. Glabrata (%5.1), and C. tropcalis .(%3.4)Likewise, C. albicans was the most frequent species (%27) in the non-diabetic individuals. In this study, the results of both methods for identification of the isolates were consistent with each other.Conclusion:Xerostomia and disturbance of physiological factors including pH and glucose can promote overgrowth of Candida flora in the oral cavity. These factors are considered important predisposing factors for oral candidiasis in diabetic patients. In the present study, it was observed that application of CHROMagar Candida and PCR-RFLP methods at the same time contributes to more accurate identification of isolates.

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Rapid identification of Candida albicans and its related species Candida stellatoidea and Candida dubliniensis by a single PCR amplification using primers specific for the repetitive sequence (RPS) of Candida albicans

Cited by 13 publications

References 18 publications

Genotyping of Candida albicans on the basis of polymorphisms of ALT repeats in the repetitive sequence (RPS)

Genotyping of Candida albicans on the basis of polymorphisms of ALT repeats in the repetitive sequence (RPS)

Genotype analysis of Candida albicans isolates obtained from different body locations of patients with superficial candidiasis using PCRs targeting 25S rDNA and ALT repeat sequences of the RPS

Identification of Candida species in the oral cavity of diabetic patients

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