Rapid identification of a narcotic plant Papaver bracteatum using flow cytometry

Aragane, Masako; Watanabe, Daisuke; Nakajima, Jun; Yoshida, Masao; Yoshizawa, Masao; Abe, Tomohiro; Nishiyama, Rei; Suzuki, Jin; Moriyasu, Takako; Nakae, Dai; Sudo, Hiroshi; Sato, Hiroyuki; Hishida, Atsuyuki; Kawahara, Nobuo; Makabe, So; Nakamura, Ikuo; Mii, Masahiro

doi:10.1007/s11418-014-0850-z

Cited by 5 publications

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“…FCM estimation of genome size has been successfully used previously for the identification of various species, such as those belonging to genera Curcuma, Nasturtium, Petunia, Lactuca, and Papaver (Koopman, 2000;Mishiba et al, 2000;Leong-Škorničková et al, 2007;Aragane et al, 2014;Morozowska et al, 2015). Also, as shown here, based on the 2C-values it was possible to identify the only tetraploid species, L. corniculatus, as well as four diploid ones, L. burttii, L. japonicus, L. maritimus, and L. uliginosus.…”

Section: Identification Of Lotus Species By Flow Cytometrymentioning

confidence: 71%

Comparison of the genome size, endoreduplication, and ISSR marker polymorphism in eight Lotus (Fabaceae) species

Ducár¹,

Rewers²,

Jędrzejczyk³

et al. 2018

Turk J Bot

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IntroductionThe genus Lotus (Fabaceae; previously Leguminosae) comprises nearly 200 annual and perennial species that are distributed worldwide, except in very cold regions and in some tropical areas of Southeast Asia and Central America (Allan et al., 2004;Escaray et al., 2012). Within this genus, more than 15% of the species are endangered, vulnerable, or rare (IUCN, 2015). Several species are important forage crops (e.g., L. corniculatus, L. uliginosus, L. tenuis, and L. subbiflorus) since their nutritional value is similar or even superior to that of white clover and alfalfa (Jones and Earle, 1966;Escaray et al., 2012).Lotus taxonomy is one of the most problematic within the tribe Loteae due to the limited number of appropriate discernable morphological traits, insufficient genetic description, and high morphological and biogeographical diversity (Grant and Small, 1996;Allan et al., 2004;Escaray et al., 2012). Therefore, in addition to morphological analyses, karyological and molecular studies should be applied for species identification and establishing taxonomic assignment and phylogenetic relationships.The majority of Lotus species have the basic chromosome number x = 6 or 7 and are mostly diploids. However, in some species both diploid and tetraploid accessions occur, while others include only tetraploid forms (e.g., L. corniculatus;Ferreira and Pedrosa-Harand, 2014). Genome sizes of the genus Lotus have been reported for 39 species only (20%), with a range from 0.56 to 2.80 pg/2C (

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Section: Identification Of Lotus Species By Flow Cytometrymentioning

confidence: 71%

Comparison of the genome size, endoreduplication, and ISSR marker polymorphism in eight Lotus (Fabaceae) species

Ducár¹,

Rewers²,

Jędrzejczyk³

et al. 2018

Turk J Bot

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show abstract

“…The relative nuclear DNA content at the vegetative stage was measured by flow cytometric (FCM) analysis in three closely related species of the genus Papaver section Oxytona, that is, P. orientale, P. pseudo-orientale, and P. bracteatum, based on the difference of the chromosome numbers (Aragane et al, 2014). The nr DNA content differed between three species, and most of the commercially distributed plants examined could be identified as P. bracteatum.…”

Section: Molecular Biologymentioning

confidence: 99%