Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals

Linton, Dennis; Owen, Robert J.; Stanley, John R.

doi:10.1016/s0923-2508(97)85118-2

Cited by 323 publications

(306 citation statements)

References 38 publications

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“…fetus is too conserved for subspecies-level differentiation, it is a reliable basis for species-level identi®cation, either by comparative analysis or by use of PCR tests based on the 16S rRNA gene sequence (e.g. Linton et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of numerical analysis of PFGE-DNA profiles for differentiating Campylobacter fetus subspecies by comparison with phenotypic, PCR and 16S rDNA sequencing methods

On¹,

Harrington²

2001

J Appl Microbiol

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Aims: To assess the ef®cacy of numerical analysis of PFGE-DNA pro®les for identi®cation and differentiation of Campylobacter fetus subspecies. Methods and Results: 31 Camp. fetus strains were examined by phenotypic, PCR-and PFGE-based methods, and the 16S rDNA sequences of 18 strains compared. Numerical analysis of PFGE-DNA pro®les divided strains into two clusters at the 86% similarity level. One cluster contained 19 strains clearly identi®ed as Camp. fetus subsp. venerealis. The other cluster comprised 12 strains, of which 10 were unambiguously identi®ed as Camp. fetus subsp. fetus. The remaining two strains were identi®ed as Camp. fetus subsp. venerealis by either phenotypic or PCR methods, but not both. At higher similarity levels, clusters containing isolates from each of two countries were identi®ed, suggesting that certain clones predominate in certain geographical regions. Conclusions: Numerical analysis of PFGE-DNA pro®les is an effective method for differentiating Camp. fetus subspecies. Signi®cance and Impact of the Study: Critical comparison of PFGE, PCR, 16S rDNA sequencing and phenotypic methods for differentiation of Camp. fetus subspecies was attained. Novel phenotypic markers for distinguishing subspecies were identi®ed. Evidence for dominant clones of each subspecies in certain countries was provided.

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Section: Discussionmentioning

confidence: 99%

Evaluation of numerical analysis of PFGE-DNA profiles for differentiating Campylobacter fetus subspecies by comparison with phenotypic, PCR and 16S rDNA sequencing methods

On¹,

Harrington²

2001

J Appl Microbiol

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“…Frozen strains at -20°C in peptone broth (Himedia/India) with 25% glycerol (Sigma-Aldrich/ USA) were thawed and spread on Agar Columbia (Himedia/India) supplemented with 0.4% activated charcoal (Vetec Fine Chemicals/Brazil) and incubated at 37°C for 48 hours in a microaerophilic atmosphere. Multiplex PCR assay using primers C1288_R C412_F for 16S rRNA gene (816 bp) specific to the genus Campylobacter (LINTON et al, 1996) and the primers C1 and C4 for oxidoreductase gene (160 bp) specific for C. jejuni (WINTERS & SLAVIK, 1995) was carried for confirmation of the identification. Primers Col1 and Col2 for the ceuE gene (894 bp) (GONZALEZ et al, 1997) were used to confirm the species C. coli.…”

Section: Methodsmentioning

confidence: 99%

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

et al. 2015

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“…Total community DNA extraction of the resulting pellets and of reference strains used as positive controls (C. jejuni LMG 8842, C. coli ATCC 33559, and C. lari ATCC 35221) was carried out using a MO BIO power soil kit (MO BIO Laboratories, Inc., Carlsbad, CA) per the manufacturer's instructions. The Campylobacter genus-specific PCR assay developed by Linton et al (23) was used to determine the presence of members of this genus, and selected amplification products were used in cloning reactions. Positive PCR products from the same sampling date were pooled, cloned into pCR4.1 TOPO (Invitrogen, Carlsbad, CA) per the manufacturer's instructions, and sequenced to determine the molecular diversity of this bacterial group.…”

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confidence: 99%

Molecular Detection of Campylobacter spp. in California Gull (Larus californicus) Excreta

Ryu

Domingo

et al. 2011

Appl Environ Microbiol

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We examined the prevalence, quantity, and diversity of Campylobacter species in the excreta of 159 California gull (Larus californicus) samples using culture-, PCR-, and quantitative PCR (qPCR)-based detection assays. Campylobacter prevalence and abundance were relatively high in the gull excreta examined; however, C. jejuni and C. lari were detected in fewer than 2% of the isolates and DNA extracts from the fecal samples that tested positive. Moreover, molecular and sequencing data indicated that most L. californicus campylobacters were novel (<97% 16S rRNA gene sequence identity to known Campylobacter species) and not closely related to species commonly associated with human illness. Campylobacter estimates were positively related with those of fecal indicators, including a gull fecal marker based on the Catellicoccus marimammalium 16S rRNA gene.

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Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals

Cited by 323 publications

References 38 publications

Evaluation of numerical analysis of PFGE-DNA profiles for differentiating Campylobacter fetus subspecies by comparison with phenotypic, PCR and 16S rDNA sequencing methods

Evaluation of numerical analysis of PFGE-DNA profiles for differentiating Campylobacter fetus subspecies by comparison with phenotypic, PCR and 16S rDNA sequencing methods

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

Molecular Detection of Campylobacter spp. in California Gull (Larus californicus) Excreta

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