Rapid Identification and Quantification of Adulteration in Methyl Eugenol using Raman Spectroscopy Coupled with Multivariate Data Analysis

Abstract

Identification of adulterants in commercial samples of methyl eugenol is necessary because it is a botanical insecticide, a tephritid male attractant lure that is used to attract and kill invasive pests such as oriental fruit flies and melon flies on crops. In this study, Raman spectroscopy was used to qualitatively and quantitatively assess commercial methyl eugenol along with adulterants. For this purpose, commercial methyl eugenol was adulterated with different concentrations of xylene. The Raman spectral features of methyl eugenol and xylene in liquid formulations were examined, and Raman peaks were identified as associated with the methyl eugenol and adulterant. Principal component analysis (PCA) and partial least-squares regression analysis (PLSR) have been used to qualitatively and quantitatively analyze the Raman spectral features. PCA was applied to differentiate Raman spectral data for various concentrations of methyl eugenol and xylene. Additionally, PLSR has been used to develop a predictive model to observe a quantitative relationship between various concentrations of adulterated methyl eugenol and their Raman spectral data sets. The root-mean-square errors of calibration and prediction were calculated using this model, and the results were found to be 1.90 and 3.86, respectively. The goodness of fit of the PLSR model is found to be 0.99. The proposed approach showed excellent potential for the rapid, quantitative detection of adulterants in methyl eugenol, and it may be applied to the analysis of a range of pesticide products.

Novel SERS Probe Based on Ag Nanobean/Cu Foam for Deep-Sea Extreme Environment Biomolecule Detection

“…Excitingly, we successfully obtained the SERS data spectrum with rich feature peaks after entering the water (Figure c). For example, the characteristic peaks at 1057 cm –1 ( o -xylene), 1226 cm –1 (stretching vibrations of CH 3 + C–H bond in plane bending + ring bending of o -xylene), 1253 cm –1 (CC stretching + ring bending of m-xylene), and 1638 cm –1 (CCH bond wagging vibration) presumed to be derived from xylene . The characteristic peaks at 1380–1409 cm –1 (ring deformation and C–C stretching) and 1526–1578 cm –1 [CC stretching (in-phase)] are more likely to originate from a variety of aromatic hydrocarbons .…”