Rapid identification and isolation of neuraminidase inhibitors from mockstrawberry (Duchesnea indica Andr.) based on ligand fishing combined with HR-ESI-Q-TOF-MS

Luo, Sifan; Guo, Lin-Bo; Sheng, Caimin; Zhao, Yan‐Xia; Chen, Ling; Li, Chufang; Jiang, Zhengjin; Tian, Hai‐Yan

doi:10.1016/j.apsb.2020.04.001

Cited by 36 publications

(13 citation statements)

References 28 publications

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“…In a docking study, vitexin was shown to have binding affinity with influenza A virus neuraminidase (Sadati et al, 2019). Similarly, the anti-influenza A virus H1N1 ability of isovitexin, with an IC 50 of 14 μg/ml, has been reported (Ye et al, 2011), and the potential mechanism is through the viral neuraminidase inhibition (Luo et al, 2020). In this study, we confirmed that VRE showed antineuraminidase activity against two different subtypes of influenza virus.…”

Section: Discussionsupporting

confidence: 71%

Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release

Pi²,

Chen

et al. 2020

Front. Pharmacol.

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Vigna radiata (L.) R. Wilczek (mung bean) is a Chinese functional food with antioxidant, antimicrobial and anti-inflammatory activities. However, little is known about its antiviral activity. We aimed to investigate the antiviral activity and mechanisms of action of Vigna radiata extract (VRE) against influenza virus. HPLC was conducted to analyze the components of the VRE. The anti-influenza viral activity of VRE in Mardin-Darby canine kidney (MDCK) cells was evaluated by virus titration assays, hemagglutination assays, quantitative RT-PCR assays, cellular α-glucosidase activity assays and neuraminidase activity assays. Chromatographic profiling analysis identified two major flavonoids, vitexin and isovitexin, in the ethanol extract of Vigna radiata. Through in vitro studies, we showed that VRE, at concentrations up to 2,000 μg/ml, exhibited no cytotoxicity in MDCK cells. VRE protected cells from influenza virus-induced cytopathic effects and significantly inhibited viral replication in a concentration-dependent manner. A detailed time-of-addition assay revealed that VRE may act on both the early and late stages of the viral life cycle. We demonstrated that 1) VRE inhibits virus entry by directly blocking the HA protein of influenza virus; 2) VRE inhibits virus entry by directly binding to cellular receptors; 3) VRE inhibits virus penetration; 4) VRE inhibits virus assembly by blocking cellular α-glucosidase activity, thus reducing HA protein trafficking to the cell surface; and 5) VRE inhibits virus release by inhibiting viral neuraminidase activity. In summary, Vigna radiata extract potently interferes with two different subtypes of influenza viruses at multiple steps during the infectious cycle, demonstrating its broad-spectrum potential as an anti-influenza preventive and therapeutic agent. Continued development of Vigna radiata-derived products into antiviral therapeutics is warranted.

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Section: Discussionsupporting

confidence: 71%

Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release

Pi²,

Chen

et al. 2020

Front. Pharmacol.

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“…Neuraminidase (NA) is a novel target for the development of therapeutic agents to treat bacterial or viral infections ( Kim et al, 2014 ; Luo et al, 2020 ). As documented in literature, jatrorrhizine showed inhibitory activity on bacterial NA with an IC 50 value of 37.0 ± 1.8 μΜ and suppressed viral NA activity against rvH1N1 and H5N1 with IC 50 values of 66.2 ± 4.2 μΜ and 76.3 ± 2.1 μΜ ( Kim et al, 2014 ).…”

Section: Pharmacological Activities Of Jatrorrhizinementioning

confidence: 99%

Jatrorrhizine: A Review of Sources, Pharmacology, Pharmacokinetics and Toxicity

Zhong

Chen

et al. 2022

Front. Pharmacol.

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Jatrorrhizine, an isoquinoline alkaloid, is a bioactive metabolite in common medicinal plants, such as Berberis vernae Schneid., Tinospora sagittata (Oliv.) Gagnep. and Coptis chinensis Franch. These plants have been used for centuries in traditional medicine for their wide-ranging pharmacological properties. This review emphasizes the latest and comprehensive information on the sources, pharmacology, pharmacokinetics and toxicity of jatrorrhizine. Studies on this alkaloid were collected from scientific internet databases, including the Web of Science, PubMed, ScienceDirect, Google Scholar, Elsevier, Springer, Wiley Online Library and Europe PMC and CNKI, using a combination of keywords involving “jatrorrhizine”, “sources”, “pharmacology,” “pharmacokinetics,” and “toxicology”. Jatrorrhizine exhibits anti-diabetic, antimicrobial, antiprotozoal, anticancer, anti-obesity and hypolipidemic properties, along with central nervous system activities and other beneficial activity. Studies of jatrorrhizine have laid the foundation for its application to the treatment of various diseases, but some issues still exist. Further investigations might emphasize 1) specific curative mechanisms of jatrorrhizine and clinical utility, 2) application prospect in the treatment of metabolic disorders, 3) comprehensive investigations of the toxicity mechanisms and 4) interactions of jatrorrhizine with other pharmaceuticals and development of derivatives.

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“…It has gradually become a powerful tool for screening biologically active components. This strategy was used for neuraminidase immobilized magnetic beads to screen inhibitors from crude extracts of mockstrawberry ( Luo et al, 2020 ) and α-glucosidase inhibitors in Epimedium ( Shen et al, 2020 ) and amyloid β (Aβ) inhibitors in Scutellaria baicalensis ( Guo et al, 2022a ). The traditional fishing strategy with a single target needs to be further enhanced to match the multi-component and multi-target characteristics of TCM ( Chen et al, 2020a ).…”

Section: Frontier Approaches and Technologies For Pharmacodynamic Sub...mentioning

confidence: 99%

Unraveling the mystery of efficacy in Chinese medicine formula: New approaches and technologies for research on pharmacodynamic substances

Lin

Wang

et al. 2022

Arabian Journal of Chemistry

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Rapid identification and isolation of neuraminidase inhibitors from mockstrawberry (Duchesnea indica Andr.) based on ligand fishing combined with HR-ESI-Q-TOF-MS

Cited by 36 publications

References 28 publications

Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release

Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release

Jatrorrhizine: A Review of Sources, Pharmacology, Pharmacokinetics and Toxicity

Unraveling the mystery of efficacy in Chinese medicine formula: New approaches and technologies for research on pharmacodynamic substances

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