Rapid Identification and Differentiation of
            <i>Trichophyton</i>
            Species, Based on Sequence Polymorphisms of the Ribosomal Internal Transcribed Spacer Regions, by Rolling-Circle Amplification

Kong, Fanrong; Tong, Zhongsheng; Chen, Xiaoyou; Sorrell, Tania C.; Wang, Bin; Wu, Qi; Ellis, David; Chen, Sharon

doi:10.1128/jcm.02235-07

Cited by 60 publications

(92 citation statements)

References 40 publications

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“…For clinical practice, a simple and robust diagnostic method is required. RCA has been applied to the identification of diverse human-pathogenic fungi (21,30,31). According to previous studies, the method proved to be easily operated, rapid, costeffective, sensitive, and specific.…”

Section: Discussionmentioning

confidence: 99%

Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

et al. 2013

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hThe species diversity and identification of black fungi belonging to Cyphellophora and Phialophora, which colonize and infect human skin and nails, were studied using amplified fragment length polymorphism (AFLP). A total of 76 Cyphellophora and Phialophora isolates were evaluated, and their delimitation was compared to earlier studies using multilocus sequencing. The results of the AFLP analysis and sequencing were in complete agreement with each other. Seven species-specific padlock probes for the most prevalent species were designed on the basis of the ribosomal DNA internal transcribed spacer region, and identification of the respective species could easily be achieved with the aid of rolling circle amplification.

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Section: Discussionmentioning

confidence: 99%

Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

et al. 2013

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“…Vorrangig ist hier die Entwicklung genetischer Untersuchungen zur Identifizierung von Pilzen zu nennen [1,17,19,20,23,35]. Mittels der Polymerasekettenreaktion können definierte Genabschnitte von Mikroorganismen amplifiziert und vergleichbar gemacht werden.…”

Section: Neue Vergleichsmethodenunclassified

“…Die letzten Jahre haben gezeigt, dass bei Dermatophyten das ribosomale Operon mit seinen Spacer-Abschnitten diesen Anforderungen auf Ebene der Artdifferenzierung am besten zu entsprechen scheint [23,35]. Viele Untersuchungen beziehen sich auf die intern transkribierten SpacerRegionen (ITS) der rDNA (.…”

Section: Microsporum-artenunclassified

Dermatophytenspezies

Brasch¹

2008

Hautarzt

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Taxonomy is an artificial system to classify living organisms. For this purpose, different concepts of how to define a species can be used. In recent years, DNA analysis has especially helped to better understand the phylogenetic relations of dermatophytes and thus influenced the distinction of dermatophyte species. In some cases species previously considered separate were made synonymous. It may be necessary to discriminate different varieties within a particular species if this is required by their typical morphologic, physiologic or epidemiological characteristics. Trichophyton eboreum with its perfect state Arthroderma olidum was recently described as a new species. The taxonomic order of dermatophytes must continuously be updated by integrating new findings; at the same time, meaningful and proven criteria should be retained.

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“…For primer design, it was important to consider the quality of the input sequence, as GenBank sequence submissions are not peer reviewed and it has been estimated that 10 to 20% of the fungal sequences in GenBank have been misidentified (26). Therefore, primers were designed from consensus sequences generated in regions devoid of intraspecies variability.…”

Section: Discussionmentioning

confidence: 99%

Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens

et al. 2008

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We describe the first development and evaluation of a rapid multiplex tandem PCR (MT-PCR) assay for the detection and identification of fungi directly from blood culture specimens that have been flagged as positive. The assay uses a short-cycle multiplex amplification, followed by 12 simultaneous PCRs which target the fungal internal transcribed spacer 1 (ITS1) and ITS2 region, elongation factor 1-␣ (EF1-␣), and ␤-tubulin genes to identify 11 fungal pathogens: Candida albicans, Candida dubliniensis, Candida glabrata, Candida guilliermondii, Candida krusei, Candida parapsilosis complex, Candida tropicalis, Cryptococcus neoformans complex, Fusarium solani, Fusarium species, and Scedosporium prolificans. The presence or absence of a fungal target was confirmed by melting curve analysis. Identification by MT-PCR correlated with culture-based identification for 44 (100%) patients. No crossreactivity was detected in 200 blood culture specimens that contained bacteria or in 30 blood cultures without microorganisms. Fungi were correctly identified in five specimens with bacterial coinfection and in blood culture samples that were seeded with a mixture of yeast cells. The MT-PCR assay was able to provide rapid (<2 h), sensitive, and specific simultaneous detection and identification of fungal pathogens directly from blood culture specimens.

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Rapid Identification and Differentiation of Trichophyton Species, Based on Sequence Polymorphisms of the Ribosomal Internal Transcribed Spacer Regions, by Rolling-Circle Amplification

Cited by 60 publications

References 40 publications

Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

Dermatophytenspezies

Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens

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