1999
DOI: 10.1128/jb.181.6.1868-1874.1999
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Rapid Hypothesis Testing with Candida albicans through Gene Disruption with Short Homology Regions

Abstract: Disruption of newly identified genes in the pathogen Candida albicans is a vital step in determination of gene function. Several gene disruption methods described previously employ long regions of homology flanking a selectable marker. Here, we describe disruption of C. albicans genes with PCR products that have 50 to 60 bp of homology to a genomic sequence on each end of a selectable marker. We used the method to disrupt two known genes,ARG5 and ADE2, and two sequences newly identified through the Candida gen… Show more

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Cited by 729 publications
(353 citation statements)
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“…C. albicans BWP171CIp30 was used for all experiments (20). This isogenic wild-type strain was selected to facilitate future comparison with C. albicans deletion mutants.…”
Section: Experimental Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…C. albicans BWP171CIp30 was used for all experiments (20). This isogenic wild-type strain was selected to facilitate future comparison with C. albicans deletion mutants.…”
Section: Experimental Methodsmentioning
confidence: 99%
“…This suggests that the observed progression of length was predominantly induced by transitions of hyphae from the non-invasive state to the invasive state, such that the net length of invaded hyphae increased at a higher overall rate [see Supporting Information S3 Eq. (20)].…”
Section: Hyphal Growth Kinetics Reveals Massive Epithelial Invasionmentioning
confidence: 99%
“…All C. albicans strains were derivatives of strain SC5314 (Gillum et al, 1984). Strain BWP17 (Wilson et al, 1999) was used to create all mutant strains. The genotypes of all strains constructed in this study are listed in Table 3.…”
Section: Strain Constructionmentioning
confidence: 99%
“…All C. albicans strains used in this study were derived from BWP17 (Wilson et al, 1999) and are listed in Table 2. Primer sequences from 5′ to 3′ are listed in Supporting Information, 'Primer sequences' worksheet.…”
Section: Construction Of Fungal Strainsmentioning
confidence: 99%