Rapid hydrophobic grid membrane filter-enzyme-labeled antibody procedure for identification and enumeration of Escherichia coli O157 in foods

Todd, Ewen C. D.; Szabo, Richard A.; Peterkin, Pearl I.; Sharpe, A. N.; Parrington, Lorna J.; Bundle, David R.; Gidney, M. A. J.; Perry, Malcolm B.

doi:10.1128/aem.54.10.2536-2540.1988

Cited by 65 publications

(27 citation statements)

References 19 publications

(13 reference statements)

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“…Escherichia coli strain O157:H7 is an enteric pathogen that can cause severe local and systemic diseases and is responsible for sporadic and for major outbreaks of haemorrhagic colitis and large numbers of haemolytic^uraemic syndrome [1]. The O157 antigen was chemically iden-ti¢ed as a polysaccharide composed of repeating tetrasaccharide units [2] having the structure: 4 À L À D À Glcp À 1 À 3 À K À D À GalpNAcÀ 1 À 2 À K À D À Rhap4NAc À 1 À 3 À K À LÀ Fucp À 1 and monoclonal antibodies directed to the antigen were made and used for the identi¢cation of the E. coli serotype O157 strains [3,4].…”

Section: Introductionmentioning

confidence: 99%

Serological cross-reaction between the lipopolysaccharide O-polysaccharide antigens of Escherichia coli O157:H7 and strains of Citrobacter freundii and Citrobacter sedlakii

Vinogradov¹

2000

FEMS Microbiology Letters

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A strain of Citrobacter sedlakii showing serological cross-reaction with Escherichia coli O157 antisera was demonstrated to produce a lipopolysaccharide O-antigen having an identical structure with that of the E. coli O157 O-antigen. A strain of Citrobacter freundii showing similar cross-reaction with E. coli O157 specific monoclonal antibody was shown to produce a lipopolysaccharide O-antigen composed of a trisaccharide repeating unit having the structure. This O-antigen differs from that of the E. coli O157 O-antigen and also lacks a component 2-substituted 4-amino-4,6-dideoxy-K-D-mannopyranosyl residue implicated as the common epitope in the lipopolysaccharide O-antigens of previously investigated bacterial species showing serological cross-reactivity with E. coli O157 antisera. The C. freundii O-antigen presents an interesting example of structural mimicry within a bacterial polysaccharide antigen. ß

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Section: Introductionmentioning

confidence: 99%

Serological cross-reaction between the lipopolysaccharide O-polysaccharide antigens of Escherichia coli O157:H7 and strains of Citrobacter freundii and Citrobacter sedlakii

Vinogradov¹

2000

FEMS Microbiology Letters

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“…Other methods developed to improve the detectability of E. coli 0157 in food and environmental samples include : the use of hydrophobic grid membranes filters (HGMF) to permit the screening of a large number of isolates (Doyle and Schoeni 1987 ;Todd et al 1988) and immunomagnetic separation (IMS) techniques for separating E. coli 0157 from the surrounding microflora (Chapman et al 1994;Mortlock 1994). This paper documents the problems encountered in the use of the above methods in the isolation of E. coli 0157 from four dairy herds and describes a protocol which isolated the target bacterium and produced the lowest proportion of false positives.…”

Section: Introductionmentioning

confidence: 99%

The use of selective and differential agars in the isolation of Escherichia coli ***O157 from dairy herds

Wallace¹,

Jones²

1996

J Appl Microbiol

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The use of selective and differential agars for the isolation of Escherichiu coli 0 157 from the faeces of dairy herds was investigated. O u t of the 614 isolates which were positive for one or more of the selective criteria employed in the media only four proved to be E. cofi 0157. Ninety-nine per cent of the isolates were false positives. T h e procedure which resulted in the isolation of E. coli 0157 from faecal samples was enrichment in modified Tryptone Soya Broth supplemented with novobiocin and subsequent growth on Chromagar@ 0 1 57.

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“…1986;Borczyk ef al. 1987;Doyle and Schoeni 1987;Todd et al 1988;March and Ratnam 1989;Okrend et al 1990;Doyle 1991;Padhye and Doyle 1991;Padhye and Doyle 1992) since its recognition as a foodborne pathogen in 1982. An antibody-direct epifluorescent filter technique has been developed for rapid direct enumeration of E. coli 0157:H7 in beef without enrichment (Tortorello and Stewart 1994).…”

Section: Introductionmentioning

confidence: 99%

MEMBRANE LIFTING TECHNIQUE FOR RAPID DETECTION OF ESCHERICHIA COLI 0157:H7 IN INOCULATED GROUND MEATS

Tsai

Slavik

1996

Rapid Methods Automation Mic

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A membrane lifting technique was developed for direct rapid detection of Escherichia coli 0157:H7 in inoculated ground meats. Duplicate groups of 2 meatballs were inoculated with volumes of 0.1‐ml of a serial dilution (1:10) of E. coli 0157:H7 or a mixed culture containing one strain of E. coli 0157:H7 and a non‐0157:H7E. coli serotype (E. coli ATCC 25922). Each meatball was sampled by sandwiching with 2 pieces of nitrocellulose membranes and pressing against each other to the center of the meatball. The membranes were in contact with the meats for 10 min to lift the bacteria. The membranes were removed and incubated on MacConkey‐sorbitol agar plates with the meat contact side up. After 18 h incubation at 37C, an immunostain was performed directly on the membranes for detection of the presence or absence of E. coli 0157:H7. This method was found to be sensitive enough to detect as few as 1 to 2 cells of E. coli 0157:H7 inoculated on surfaces of 18‐g meatballs. This method might be used as a rapid presumptive test for E. coli 0157:H7.

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Rapid hydrophobic grid membrane filter-enzyme-labeled antibody procedure for identification and enumeration of Escherichia coli O157 in foods

Cited by 65 publications

References 19 publications

Serological cross-reaction between the lipopolysaccharide O-polysaccharide antigens of Escherichia coli O157:H7 and strains of Citrobacter freundii and Citrobacter sedlakii

Serological cross-reaction between the lipopolysaccharide O-polysaccharide antigens of Escherichia coli O157:H7 and strains of Citrobacter freundii and Citrobacter sedlakii

The use of selective and differential agars in the isolation of Escherichia coli ***O157 from dairy herds

MEMBRANE LIFTING TECHNIQUE FOR RAPID DETECTION OF ESCHERICHIA COLI 0157:H7 IN INOCULATED GROUND MEATS

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