Rapid high-performance liquid chromatography of 3-methylhistidine in human urine

Minkler, Paul E.; Ingalls, Stephen T.; Griffin, Ronda; Hoppel, Charles L.

doi:10.1016/0378-4347(87)80211-6

Cited by 11 publications

(3 citation statements)

References 36 publications

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“…At the end of the daily urine collection, we gathered a urine sample from the pooled urine after immixture of the collected urine and stored at À80 C until processing. The 3-MH concentration was determined by highperformance liquid chromatography 15 (SRL, Inc). The value of 3-MH and Cre in urine samples was multiplied by the 24-hour urine volume to produce a value for daily 3-MH/Cre excretion.…”

Section: Discussionmentioning

confidence: 99%

Neuromuscular electrical stimulation may attenuate muscle proteolysis after cardiovascular surgery: A preliminary study

Iwatsu

Iida

Kono

et al. 2017

The Journal of Thoracic and Cardiovascular Surgery

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Section: Discussionmentioning

confidence: 99%

Neuromuscular electrical stimulation may attenuate muscle proteolysis after cardiovascular surgery: A preliminary study

Iwatsu

Iida

Kono

et al. 2017

The Journal of Thoracic and Cardiovascular Surgery

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“…3-Methylhistidine was isolated from the resulting solution by ion-exchange chromatography and quantified by HPLC as described by Minkler et al 32 Plasma bilirubin and creatinine concentrations and activities of alkaline phosphatase and aspartate aminotransferase were determined on an S-Plus STKR autoanalyzer (Coulter Counter Instruments, Hialeah, FL). Urine creatinine concentrations were determined spectrophotometrically.…”

Section: Materials and Equipmentmentioning

confidence: 99%

“…Peptide-linked trimethyllysine and free trimethyllysine contents in tissues and carcass were determined by high-performance liquid chromatography (HPLC) as described by Davis et al 28 Trimethyllysine and N-acetyl-trimethyllysine content in plasma and urine were determined by HPLC as reported by Minkler et al 30 Butyrobetaine content in carcass and urine was determined by the radioenzymatic method described by Sandor et al 31 For the determination of 3-methylhistidine content, carcass, skeletal muscle, or urine was treated with 6 mol/L HCl (final concentration) at 105°C for 18 hours. 3-Methylhistidine was isolated from the resulting solution by ion-exchange chromatography and quantified by HPLC as described by Minkler et al 32 Plasma bilirubin and creatinine concentrations and activities of alkaline phosphatase and aspartate aminotransferase were determined on an S-Plus STKR autoanalyzer (Coulter Counter Instruments, Hialeah, FL). Urine creatinine concentrations were determined spectrophotometrically.…”

Section: Materials and Equipmentmentioning

confidence: 99%

Decreased carnitine biosynthesis in rats with secondary biliary cirrhosis

2000

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Carnitine biosynthesis was investigated in rats with secondary biliary cirrhosis induced by bile duct ligation (BDL) for 4 weeks (n ‫؍‬ 5) and in pair-fed, sham-operated control rats (n ‫؍‬ 4). Control rats were pair-fed to BDL rats, and all rats were fed an artificial diet with negligible contents of carnitine, butyrobetaine, or trimethyllysine. Biosynthesis of carnitine and its precursors was determined by measuring their excretion in urine and accumulation in the body of the animals. Four weeks after BDL, total carnitine content was increased by 33% in livers from BDL rats when compared with control rats, but was unchanged in skeletal muscle and whole carcass. The plasma total carnitine concentration averaged 29.0 ؎ 4.1 vs. 46.4 ؎ 7.3 mol/L in BDL rats and control rats, respectively. Urinary total carnitine excretion was reduced by 56% in BDL rats as compared with control rats. Carnitine biosynthesis was significantly decreased in BDL rats (0.45 ؎ 0.19 vs. 0.93 ؎ 0.08 mol/100 g body weight/d in BDL and control rats, respectively). The tissue content of free and protein-linked trimethyllysine, a carnitine precursor, and trimethyllysine plasma concentrations were not different between BDL and control rats. However, urinary trimethyllysine excretion was increased 5-fold in BDL rats and approximated glomerular filtration. In contrast, urinary excretion of butyrobetaine, the direct carnitine precursor, was decreased by 40% in BDL rats as compared with control rats. Trimethyllysine biosynthesis was not different, but butyrobetaine biosynthesis was decreased by 51% in BDL as compared with control rats. In conclusion, carnitine biosynthesis is decreased in BDL rats as a result of a defect in the conversion of trimethyllysine to butyrobetaine.

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A sensitive and specific high performance liquid chromatographic assay for imidazole dipeptides and 3‐methylhistidine in human muscle biopsies, serum and urine

Teahon

Rideout

1992

Biomedical Chromatography

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Rapid high-performance liquid chromatography of 3-methylhistidine in human urine

Cited by 11 publications

References 36 publications

Neuromuscular electrical stimulation may attenuate muscle proteolysis after cardiovascular surgery: A preliminary study

Neuromuscular electrical stimulation may attenuate muscle proteolysis after cardiovascular surgery: A preliminary study

Decreased carnitine biosynthesis in rats with secondary biliary cirrhosis

A sensitive and specific high performance liquid chromatographic assay for imidazole dipeptides and 3‐methylhistidine in human muscle biopsies, serum and urine

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