Rapid high-performance liquid chromatographic method for the determination of dapsone and monoacetyldapsone in biological fluids

Zuidema, J.; Modderman, E. S. M.; Hilbers, Hans W.; Merkus, F. W. H. M.; Huikeshoven, H

doi:10.1016/s0378-4347(00)81662-x

Cited by 16 publications

(5 citation statements)

References 8 publications

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“…The predominant route of elimination of dapsone is metabolism, with only 5% to 15% of an administered dose found in the urine as unchanged drug in adults. 5 Dapsone is extensively metabolized by both oxidative and conjugative processes, forming dapsone hydroxylamine and the N-acetylated metabolites monoacetyldapsone and hydroxymonoacetyldapsone. 14 Deacetylation of the acetylated metabolites also takes place, and an equilibrium between acetylation and deacetylation is reached within a few hours after dapsone administration, resulting in a relatively constant acetylation ratio.…”

Section: Discussionmentioning

confidence: 99%

“…Dapsone assay. Serum dapsone and monoacetyldapsone concentrations were determined by HPLC with use of a modification of the method of Zuidema et al 5 HPLC was performed by use of a Biorad 800 HRLC, with a Supelcosil LC 18 column (15 cm × 4.6 mm) maintained at 35°C. The mobile phase consisted of 16% acetonitrile and 84% acetic acid (1.5%) delivered at a flow rate of 2.2 mL/min.…”

Section: Methodsmentioning

confidence: 99%

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Population pharmacokinetics of dapsone in children with human immunodeficiency virus infection

Mirochnick

Cooper

Capparelli

et al. 2001

Clin Pharma and Therapeutics

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Ethnicity, age, and concomitant rifabutin use were associated with dapsone CL/F, with more rapid clearance observed in black children, children younger than 2 years old, and children receiving rifabutin. Dapsone pharmacokinetic parameters were not associated with toxicity, but higher dapsone AUC was associated with decreased risk of PCP. Monitoring of serum dapsone levels may be needed for optimal management of dapsone for PCP prophylaxis in children.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Population pharmacokinetics of dapsone in children with human immunodeficiency virus infection

Mirochnick

Cooper

Capparelli

et al. 2001

Clin Pharma and Therapeutics

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“…Dapsone assay. Serum dapsone and monoacetyldapsone (MADDS) concentrations were determined by high-performance liquid chromatography (HPLC) by a modification of the method of Zuidema et al (19). HPLC was performed with a Bio-Rad 800 HRLC chromatograph with a Supelcosil LC-18 column (15 cm by 4.6 mm) maintained at 35°C.…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of Dapsone Administered Daily and Weekly in Human Immunodeficiency Virus-Infected Children

Mirochnick

Cooper

McIntosh

et al. 1999

Antimicrob Agents Chemother

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Although dapsone is a commonly used alternative agent for prophylaxis against Pneumocystis carinii pneumonia in children intolerant to trimethoprim-sulfamethoxazole, there are few data that describe dapsone pharmacokinetics in children. We studied dapsone pharmacokinetics in 30 children (median age, 2.8 years; age range, 0.3 to 12 years) receiving a new proprietary liquid preparation by three dosing regimens (1 mg/kg of body weight daily, 2 mg/kg daily, or 4 mg/kg weekly). Dosing of children with 2 mg/kg daily or 4 mg/kg weekly resulted in peak concentrations equivalent to those reached in adults receiving 100-mg tablets daily. For the entire population, the median half-life was 22.2 h (range, 7.1 to 40.3 h), the median oral clearance was 0.0365 liter/kg/h (range, 0.0104 to 0.1021 liter/kg/h), and the median oral apparent volume of distribution was 1.13 liters/kg (range, 0.50 to 2.32 liters/kg). The median dapsone oral clearance was significantly increased in those infants less than 2 years of age compared to the oral clearance in those over 2 years of age (0.0484 versus 0.0278 liter/kg/h; P = 0.011). These data suggest that absorption of this liquid preparation is adequate and that the concentrations in the sera of children receiving 2 mg/kg daily or 4 mg/kg weekly are equivalent to those seen in adults receiving standard dapsone dosing. Dapsone oral clearance appears to be increased in children under 2 years of age.

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“…HPLC of the internal standard adopted; monopropionyldapsone. Other H P L C procedures (4,15) lack the simplicity and require complex sample preparation. T h e method described by Philip et al (16), used no internal standard and had the disadvantage of inadequate resolution from the endogenous plasma components in some samples.…”

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

“…This emphasizes the importance of devising a sensitive and specific method for the determination of D D S and MAD in biological fluids. Several methods have been reported for the estimation of D D S and MAD including colorimetric (7)(8)(9), fluorometric (10-1 l), thin layer chromatographic (12) and high performance liquid chromatographic (4, [13][14][15][16] techniques. This report describes a simple, rapid, reproducible one-stage protein precipitation H P L C method for the simultaneous determination of D D S and MAD in plasma and urine using sulphamethazine (SMZ) as an internal standard.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous High Performance Liquid Chromatographic Determination of Dapsone and Monoacetyldapsone in Human Plasma and Urine

Al-Hadidi

et al. 2008

Journal of Clinical Pharmacy and Therapeutics

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A rapid, specific and a one-stage protein precipitation method for simultaneous estimation of dapsone (DD S) and monoacetyldapsone (MAD) concentration in plasma and urine using high performance liquid chromatography (HPLC) is described. T h e applicability of the method for monitoring D D S and MAD blood levels in two different acetylator phenotype volunteers following the administration of 100-mg oral dose of D D S was shown. Cumulative urinary excretion of D D S and MAD were studied in the same volunteers.Correspondence: D r M. Abuirjeie,

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Rapid high-performance liquid chromatographic method for the determination of dapsone and monoacetyldapsone in biological fluids

Cited by 16 publications

References 8 publications

Population pharmacokinetics of dapsone in children with human immunodeficiency virus infection

Population pharmacokinetics of dapsone in children with human immunodeficiency virus infection

Pharmacokinetics of Dapsone Administered Daily and Weekly in Human Immunodeficiency Virus-Infected Children

Simultaneous High Performance Liquid Chromatographic Determination of Dapsone and Monoacetyldapsone in Human Plasma and Urine

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