Rapid high-performance liquid chromatographic identification/quantification of total vitamin C in fruit drinks

Furusawa, Naoto

doi:10.1016/s0956-7135(00)00021-9

Cited by 47 publications

(29 citation statements)

References 7 publications

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“…All fruit samples were reduced with 50 mM DTT (to reduce dehydroascorbic acid in order to determine total ascorbic acid content) and centrifuged at 5600 RCF (9g), 4°C for 10 min [17]. The supernatant (soluble fraction) was filtered through a 0.45-lm nylon filter (PALL) prior to injection into HPLC.…”

Section: Methodsmentioning

confidence: 99%

Effect of environmental factors [air and UV-C irradiation] on some fresh fruit juices

Rameshkumar

Thilagar

Jeyadevi

et al. 2012

Eur Food Res Technol

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The present study was carried out to evaluate the effect of environmental factors (air and UV-C irradiation) on various common fruits juices (lemon, orange, papaya and grape). Total phenolics, total ascorbic acid (AA) content and antioxidant capability were determined in atmospheric air oxidation on fresh fruit juices, and the effect of UV-C irradiation on AA was investigated in both fresh fruit juices and commercial AA using RP-HPLC. The total phenolics content was found to be 92.46 ± 1.5, 41.53 ± 2.1, 138.56 ± 2.3 and 110.54 ± 3.9 mg/100 ml of lemon, orange, papaya and grape juices, respectively. DPPH radical-scavenging activity of the fresh juices was 94.53 ± 3.1, 82.36 ± 2.2, 94.65 ± 3.5 and 90.74 ± 2.6 percentage, whereas the 1-day-old fruit juices showed reduced scavenging activity when compared to fresh juices. Total phenolic (TP) and DPPH-scavenging activity were significantly decreased in the mean at P \ 0.05 level. Ascorbic acid (vitamin C) content of fresh fruit juices was determined through RP-HPLC; fruit juices that were kept in open container for 24 hrs and that were treated with UV irradiation have shown a decrease in AA content, when compared with control fresh juice samples. AA degradation increased with high significant differences at P \ 0.001 level, with the increase in oxidation time in atmospheric air and UV-C radiation.

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Section: Methodsmentioning

confidence: 99%

Effect of environmental factors [air and UV-C irradiation] on some fresh fruit juices

Rameshkumar

Thilagar

Jeyadevi

et al. 2012

Eur Food Res Technol

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“…The classic method is the titulometric that does not quantify DHA but only AA. 13 Spectrophotometry, 14 fluorimetry, 10,15 amperometry, 16 electrophoresis, 17,18 chromatography [7][8][9]19 and enzymatic methods 20 have also been used, but some of these methods have limitations regarding specificity. High-performance liquid chromatography (HPLC) stands out as a reliable and usually simple method.…”

Section: Introductionmentioning

confidence: 99%

“…After separation in chromatography column, the use of a diode array detector can improve the reliability of AA identification. 19 As DHA detection is hindered by its weak molar absortivity, 1 most researchers choose to reduce it to AA before chromatographic separation, making indirect quantification by difference. Among the reducing agents dithiothreitol (DTT) has been widely used.…”

Section: Introductionmentioning

confidence: 99%

“…Among the reducing agents dithiothreitol (DTT) has been widely used. 6,19,21,22 The objective of this study was to test the methodology for analyzing AA and DHA in vegetables by reverse phase high performance liquid chromatography, optimizing the extraction processes of ascorbic acid and DHA conversion to ascorbic acid, using dithiothreitol (DTT). In addition, the efficiency of the optimized methodology was verified in vegetables with the addition of oil.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of methodology to analyze ascorbic and dehydroascorbic acid in vegetables

Campos¹,

Ribeiro²,

Lúcia³

et al. 2009

Quím. Nova

106

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OptimizatiOn Of methOdOlOgy tO analyze ascOrbic and dehydrOascOrbic acid in vegetables flávia milagres campos*, sônia machado rocha ribeiro, ceres m. della lucia e helena maria pinheiro-sant'ana Departamento de Nutrição e Saúde, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36571-000 Viçosa -MG, Brasil paulo césar stringheta Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36571-000 Viçosa -MG, BrasilRecebido em 10/1/08; aceito em 18/8/08; publicado na web em 15/12/08In this study, different solutions to extract vitamin C were tested. High-performance liquid chromatography was chosen and the conditions were based on isocratic elution in reverse phase column. Dehydroascorbic acid was determined indirectly after its reduction using dithiothreitol. The use of metaphosphoric acid to stabilize the vitamin C was shown to be required and it was necessary to neutralize the pH of the extract to apply dithiothreitol. The average recovery was 90% in collard and tomato samples. The presence of oil did not interfere in extraction and the methodology can be used to analyze stir fried vegetables.

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“…The tert-butyldimethylsilyl derivatives of AA and DHA were characterized by gas chromatography-mass spectrometry, and an isotope dilution assay for AA and DHA was developed [36]. In literature are reported several chromatographic [37 -48] and capillary electrophoresis [37,38,49] AA and DHA separative procedures in pharmaceuticals. However, the methods require the derivatization prior [44 -48,50, 51] or after [41,43,46] to HPLC separation.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Detection of Ascorbic Acid and Dehydroascorbic Acid Using Hydrodynamic Amperometry and Anodic Stripping Voltammetry on Modified Aluminum Electrodes

2010

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An electroanalytical strategy for the simultaneous determination of ascorbic acid (AA) and dehydroascorbic acid (DHA), is described. A palladized Al electrode is used for hydrodynamic amperometry of AA. While the decrease of anodic stripping voltammetry current of the K 2 UO 2 [Fe(CN) 6 ]-Pd/Al electrode prepared in the presence of DHA was the principal of the DHA determination. The calibration graph for both methods was linear over the concentration range 1 -50 mM. The detection limit was found to be 0.5 mM. Some fresh fruit juices and vegetables of trace level of AA and DHA were analyzed as the typical example of application.

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Rapid high-performance liquid chromatographic identification/quantification of total vitamin C in fruit drinks

Cited by 47 publications

References 7 publications

Effect of environmental factors [air and UV-C irradiation] on some fresh fruit juices

Effect of environmental factors [air and UV-C irradiation] on some fresh fruit juices

Optimization of methodology to analyze ascorbic and dehydroascorbic acid in vegetables

Differentiation of Detection of Ascorbic Acid and Dehydroascorbic Acid Using Hydrodynamic Amperometry and Anodic Stripping Voltammetry on Modified Aluminum Electrodes

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