Rapid high-performance liquid chromatographic determination of docetaxel (Taxotere) in plasma using liquid–liquid extraction

Ciccolini, Joseph; Catalin, J; Blachon, M.F; Durand, Alain

doi:10.1016/s0378-4347(01)00238-9

Cited by 55 publications

(28 citation statements)

References 15 publications

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“…At the assay's lower limit of quantitation (0.50 nM; 400 pg/ml), accuracy was 103% and between-run precision was 17.5%. This represents a 25-50-fold increase in sensitivity compared with analytical assays based on high-performance liquid chromatography with UV detection (22)(23)(24)(25)(26)(27)(28), although an analytical assay based on high-performance liquid chromatography with mass spectrometric detection with an lower limit of quantitation of 0.30 nM has recently been described (29). For quantitation of docetaxel in unknown samples, quality control samples at low, medium, and high concentrations were assayed in duplicate and were distributed among the calibrators and unknown samples in the analytical run; no more than 33% of the quality assurance samples were greater than Ϯ15% of the nominal concentration.…”

Section: Methodsmentioning

confidence: 99%

Comparative Pharmacokinetics of Weekly and Every-Three-Weeks Docetaxel

Baker

Zhao

Lee

et al. 2004

Clinical Cancer Research

114

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Section: Methodsmentioning

confidence: 99%

Comparative Pharmacokinetics of Weekly and Every-Three-Weeks Docetaxel

Baker

Zhao

Lee

et al. 2004

Clinical Cancer Research

114

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“…In the current method, tizanidine was extracted from plasma using liquid extraction which is extensively for determination of low drug concentrations in biological fluids (Ciccolini et al, 2001;Esrafili et al, 2007;Xiong et al, 2009). Gan et al (2002) developed and validated the estimation of tramadol in human plasma using HPLC followed by liquid-liquid extraction.…”

Section: Resultsmentioning

confidence: 99%

Development and validation of a reverse phase high performance liquid chromatography (HPLC) method for determination of tizanidine in human plasma

Ali¹,

Shoaib²,

Yousuf³

et al. 2014

Afr. J. Pharm. Pharmacol.

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A simple and cost effective high performance liquid chromatography (HPLC) method was developed for determination of tizanidine in human plasma using liquid extraction technique. The assay of tizanidine was performed after extraction of drug from plasma using diethyl ether as extraction solvent. The isocratic elution was performed in Agilent, Zorbax SB-C 18 , 4.6 × 150 mm column maintained at 30°C with mobile phase containing acetonitrile and ammonium acetate in a ratio of 15:85 v/v, respectively. The linear relationship was found within the concentration range of 0.25 to 8 ng/ml, with a flow rate of 1 ml/min and detector wavelength of 230 nm. The evaluated validation parameters were found within the acceptable range. Use of simple HPLC technique with short retention time makes this method a convenient choice for assay of tizanidine in human plasma.

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“…The mobile phase consisted of 65 parts of acetonitrile and 35 parts of double distilled water. The flow rate was 1 ml/min and the detection was performed at l max of 227 nm (Ciccolini et al, 2001).…”

Section: Determination Of Total Drug Content and Entrapment Efficiencymentioning

confidence: 99%

“…Each docetaxel-loaded LNE (0.1 ml) was diluted to 1 ml and further diluted twice (1:10) with methanol, and 20 ml sample was injected to determine drug content by the reported HPLC method (Ciccolini et al, 2001). …”

Section: Drug Contentmentioning

confidence: 99%

Albumin anchored docetaxel lipid nanoemulsion for improved targeting efficiency – preparation, characterization, cytotoxic, antitumor and in vivo imaging studies

et al. 2015

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The aim was to develop albumin anchored docetaxel lipid nanoemulsion (ALNE) for improving tumor targeted delivery. The O/W lipid nanoemulsion, LNEs were prepared by homogenization and ultrasonication processes. The size of globules and zeta potential were measured by Malvern Zetasizer. Albumin was coupled to stearylamine containing lipid nanoemulsion (SALNE) globules using water soluble EDC reaction. The drug content and entrapment efficiencies for the LNEs were determined by the high-performance liquid chromatography. The in vitro cytotoxic studies of the delivery systems were performed on MCF-7 and Hela cells. The IC 50 values of ALNE on both the cell lines were statistically significant. The in vivo antitumor activity was tested on solid tumors induced in C57BL/6 mice. This study revealed that the percentage tumor inhibition for the groups treated with DLNE, SALNE and ALNE when compared with untreated control was found to be 55.62 ± 5.41%, 54.27 ± 4.85% and 80.01 ± 2.74%, respectively. Furthermore, in vivo distribution studies were carried out in breast cancer MDA-MB231 xenografted Balb/c mice. The LNEs were loaded with fluorescent DiD oil and the distribution in different organs after 6 h was tracked using Caliper life sciences in vivo imaging system. The studies revealed that ALNE was superior in tumor targeting activity when compared with DLNE and SALNE by 3.04 and 2.26 folds, respectively. The average radiance values of ALNE on the tumor tissue were statistically significant when compared with DLNE, SALNE at p50.01. In addition, this strategy can become a platform technology for other lipophilic drugs to target tumors. KeywordsAlbumin tagged docetaxel lipid emulsion, antitumor study, fluorescent imaging study, improved tumor delivery, tumor targeting History

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Rapid high-performance liquid chromatographic determination of docetaxel (Taxotere) in plasma using liquid–liquid extraction

Cited by 55 publications

References 15 publications

Comparative Pharmacokinetics of Weekly and Every-Three-Weeks Docetaxel

Comparative Pharmacokinetics of Weekly and Every-Three-Weeks Docetaxel

Development and validation of a reverse phase high performance liquid chromatography (HPLC) method for determination of tizanidine in human plasma

Albumin anchored docetaxel lipid nanoemulsion for improved targeting efficiency – preparation, characterization, cytotoxic, antitumor and in vivo imaging studies

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