Rapid high-performance liquid chromatographic assay of acetaminophen in serum and tissue homogenates

Colin, Patrick; Sirois, G; Chakrabarti, Swapan

doi:10.1016/0378-4347(87)80222-0

Cited by 21 publications

(7 citation statements)

References 18 publications

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“…Although it is debatable whether the paracetamol/dextropropoxyphene combination is superior to paracetamol alone,1, 2 nevertheless, such combination products have been widely used. Numerous analytical methods for plasma paracetamol concentration determination have been reported 3–10. The majority of these are based on high‐performance liquid chromatography (HPLC) 5–10.…”

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confidence: 99%

“…Numerous analytical methods for plasma paracetamol concentration determination have been reported 3–10. The majority of these are based on high‐performance liquid chromatography (HPLC) 5–10. For dextropropoxyphene, a number of analytical methods including HPLC with UV11, 12 or electrochemical detection,13, 14 gas chromatography (GC)15–17 and liquid chromatography/mass spectrometry (LC/MS),18 have been described.…”

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Simultaneous determination of paracetamol and dextropropoxyphene in human plasma by liquid chromatography/tandem mass spectrometry: application to clinical bioequivalence studies

Yin

Lam

Chow

2005

Rapid Comm Mass Spectrometry

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A liquid chromatography/mass spectrometry method for simultaneous determination of paracetamol and dextropropoxyphene in human plasma is described. Paracetamol and dextropropoxyphene, together with their internal standards (tolbutamide and pyrroliphene), were extracted from 0.5 mL of plasma using solid-phase extraction. The chromatography was performed using a Thermo Hypersil APS-2 Amino column (250 mm x 4.6 mm, 5 microm) with a mobile phase consisting of acetonitrile and 0.4% glacial acetic acid in water (20:80). The total run time was 6 min for each sample. The triple-quadrupole mass spectrometer was operated in both positive (for detection of dextropropoxyphene and its IS pyrroliphene) and negative (for detection of paracetamol and its IS tolbutamide) modes using a polarity-switching technique. Multiple reaction monitoring was used for quantification. The method was linear over the concentration range of 0.1-20 microg/mL for paracetamol and 0.5-80 ng/mL for dextropropoxyphene. The intra- and inter-day precision were less than 10%, and the accuracy ranged from 92.2-110.9%. The lower limits of quantification were 0.1 microg/mL for paracetamol and 0.5 ng/mL for dextropropoxyphene. The present method provides a robust, fast and sensitive analytical tool for both paracetamol and dextropropoxyphene, and has been successfully applied to a clinical bioequivalence study in 14 subjects.

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Simultaneous determination of paracetamol and dextropropoxyphene in human plasma by liquid chromatography/tandem mass spectrometry: application to clinical bioequivalence studies

Yin

Lam

Chow

2005

Rapid Comm Mass Spectrometry

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“…Colin et al. [40], after a single intraperitoneal dose of 750 mg/kg, obtained in one Sprague–Dawley rat an absorption constant rate of 2.7/h and an apparent volume of distribution of 1.2 L/kg. Hirate et al.…”

Section: Discussionmentioning

confidence: 99%

Influence of simulated weightlessness on the pharmacokinetics of acetaminophen administered by the oral route: a study in the rat

Gandia¹,

Saivin²,

Lavit³

et al. 2004

Fundamemntal Clinical Pharma

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During space flights, the human body is submitted to weightlessness which induces physiological variations that could modify drug disposition during space missions. Since space experiments are infrequent and difficult to perform, in order to evaluate pharmacokinetic modifications, simulation experiments of weightlessness have to be carried out on earth, using animal-models such as the Morey-Holton model. In this model, rats are suspended by the tail with their front paws on the ground. We studied the effects of simulated weightlessness on drug absorption and on gastric emptying, using acetaminophen as a probe. Three periods of suspension (1, 2 and 5 days) were compared with two control groups (free and attached rats). The attached group was used to evaluate a possible 'stress effect' caused by the suspension device. Each group was composed of 36 rats (12 sampling times and three rats per time). An oral dose of acetaminophen (100 mg/kg) was administered and blood samples were collected before and up to 12 h after administration. Plasma assays were performed using an high-performance liquid chromatography method with UV detection. The calculated population pharmacokinetic parameters were Ka, Kel (first order absorption and elimination constants) and Vd/F (apparent volume of distribution). The statistical interpretation of the population pharmacokinetic parameters indicated that 2 days of suspension significantly decreased the Vd/F by 83% and the Ka by 125%. The increase in the Ka was probably because of an increased acceleration of the gastric emptying and/or to a decrease in the total peripheral resistance which increased intestinal blood flow.

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“…Cephalexin was analysed with cefoxitin as internal standard according to a published procedure (Charles & Ravenscroft 1984). Paracetamol was analysed essentially according to a published procedure (Colin & Sirois 1987).…”

Section: Sample Analysismentioning

confidence: 99%

Estimation of Oral Bioavailability in the Rat by the Accelerated Infusion Technique

Guo¹,

Luo²,

Dobson³

et al. 2000

Journal of Pharmacy and Pharmacology

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Rapid high-performance liquid chromatographic assay of acetaminophen in serum and tissue homogenates

Cited by 21 publications

References 18 publications

Simultaneous determination of paracetamol and dextropropoxyphene in human plasma by liquid chromatography/tandem mass spectrometry: application to clinical bioequivalence studies

Simultaneous determination of paracetamol and dextropropoxyphene in human plasma by liquid chromatography/tandem mass spectrometry: application to clinical bioequivalence studies

Influence of simulated weightlessness on the pharmacokinetics of acetaminophen administered by the oral route: a study in the rat

Estimation of Oral Bioavailability in the Rat by the Accelerated Infusion Technique

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