Rapid Growth Inhibition of <i>Avena</i> Coleoptile Segments by Abscisic Acid

Rehm, Marilyn M.; Cline, Morris G.

doi:10.1104/pp.51.1.93

Cited by 41 publications

(14 citation statements)

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“…The transport (9) and distribution (3) pattern of cAMP in certain plant tissues is also similar to that of auxin. On the other hand some investigators report that cAMP alone does not promote growth in dwarf pea stems (18) very rapid promotion of elongation in Avena coleoptiles (7,21). In the present experiments, growth of 1-cm segments from coleoptiles of 4-to 5-day-old Avena sativa L. cv.…”

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confidence: 62%

The Lack of Effect of Cyclic Adenosine 3′:5′-Monophosphate on Avena Coleoptile Growth

Edgerton¹,

Cline²,

Rehm³

1975

Plant Physiol.

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The effects of cyclic adenosine 3': 5'-monophosphate (cAMP) on the growth of Arena coleoptile segments over 4 to 10 hours were monitored with a position sensing transducer. At pH 6, cAMP (0.1 mM with and without 2.5 mM glucose; or 2 mM alone) or dibutyryl cAMP (0.1 mM) was added at the beginning of the experiment, or after about 1 hour or after about 6 or 7 hours. Under all conditions tested, cAMP compounds had little or no effect on coleoptile segment elongation. Inasmuch as cAMP does not duplicate the rapid and vigorous elongation obtained with 2 gM auxin, the hypothesis that cAMP is a mediator of auxin activity is not supported by experimental evidence in this system. This conclusion is dependent upon the assumption that the cAMP compounds penetrated the tissue.

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confidence: 62%

The Lack of Effect of Cyclic Adenosine 3′:5′-Monophosphate on Avena Coleoptile Growth

Edgerton¹,

Cline²,

Rehm³

1975

Plant Physiol.

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“…In contrast, ABA induced significant growth inhibition in each coleoptile portion, for every tested concentration (10, 16,25) for both in vivo and in vitro treatments. The last point could be explained by the fact that ABA is possibly not formed (15) by the coleoptile tissues, which was of course not the case for IAA.…”

Section: Resultsmentioning

confidence: 92%

“…The most sensitive zone of the material-for hormonal treatments-WM first localized and then comparatively cultured both in situ and in vitro. Growth stimulation by IAA was nearly proportional to its concentration up to 10~* M, while ABA always induced an significant inhibition.The inhibitory effect of abscisic acid (ABA) on growth is now clearly established (see 15), and its interactions with indolyl-3-acetic acid (IAA) have been studied using several parameters (21,22,25).It was of interest to analyse the growth pattern of intact coleoptiles treated with IAA and ABA and to compare the results obtained with those given by a usual coleoptile segment test (14, 24). …”

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confidence: 99%

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Critical study of coleoptile elongation controlled by IAA and ABA I. Growth kinetics and distribution

Schlienger

Hofer

Pilet

1977

Plant and Cell Physiology

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The elongation rate of wheat coleoptiles, treated with IAA and ABA, was already affected during the first 8 hr of culture. The most sensitive zone of the material-for hormonal treatments-WM first localized and then comparatively cultured both in situ and in vitro. Growth stimulation by IAA was nearly proportional to its concentration up to 10~* M, while ABA always induced an significant inhibition.The inhibitory effect of abscisic acid (ABA) on growth is now clearly established (see 15), and its interactions with indolyl-3-acetic acid (IAA) have been studied using several parameters (21,22,25).It was of interest to analyse the growth pattern of intact coleoptiles treated with IAA and ABA and to compare the results obtained with those given by a usual coleoptile segment test (14, 24). Material and methodsCaryopses of Triticum vulgare L., cv. Probus were soaked in water for 2 hr, carefully washed and sown on moist filter paper, as previously described (7). After 72 hr (dark; 25± 1°C), only seedlings with 20^1 rnm coleoptiles were selected.In situ experiments: The surface of the coleoptiles was carefully marked into 1 mm segments with India ink, and 20 intact seedlings were placed in Petri dishes on moist filter paper for 4 hr. Then, after careful washing, they were transferred (zero time) onto active solutions (still on filter paper).In vitro experiments: According to a usual technique (17, 19), coleoptile segments of 5 mm in length were excised at 5 mm from the tip. After 4 hr incubation in water (zero time), they were immersed in active solutions.Active solutions: Phosphate-citrate buffer solutions at pH 5.4 (17) containing 2% sucrose were used with or without IAA or ABA at several concentrations.All active solutions and materials (Petri dishes, filters, etc.,) employed were previously sterilized. All manipulations were conducted under dim green light.Changes of elongation were analyzed using a photographic technique (26). Each result thus represents the mean response of at least 60 measurements. Standard errors of this mean have been calculated and significant differences assessed by the <-test.Abbreviations: IAA, indolyl-3-acetic acid; ABA, abscisic acid. 729

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“…An angular position-sensing transducer was used to produce continuous elongation versus time curves. The curves were analyzed by computer and replotted by an IBM 1130 line plotter as previously described (20). Solutions containing inhibitors were added as described under "Results."…”

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Cyanide Inhibition of Acid-induced Growth in Avena Coleoptile Segments

Cline

Evans²,

Rehm³

1979

Plant Physiol.

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The comparative effects of metabolic inhibitors on acid-and auxininduced growth in oat (Avena sativa L. var. Victory) coleoptile segments have been examined. Acid (pH 4)-induced growth in both peeled and unpeeled segments is inhibited by 1 millimolar KCN when added at the time of acidification. KCN inhibits total acid-induced growth by 59 and 76%, respectively, in peeled and nonpeeled segments during the first 60 minutes. The growth rate of cyanide-treated tissue drops to zero or near zero in both peeled and nonpeeled segments during this period. Cyanide inhibition of total acid-induced growth in peeled segments at pH 5 is even more severe, amounting to about 80% during the first 60 mniutes. The possibility that inhibition by cyanide may be caused by some nonspecific effect of the inhibitor on a process other than respiration, e.g. turgor reduction due to membrane damage, has not been rled out. Acidinduced growth is also inhibited by 3 millimolar sodium fluoride and by anoxia. In unpeeled segments total pH 4-induced growth is inhibited 73% by sodium fluoride and 38% by anoxia during the 1st hour. Possible corrections to the above inhibition percentages which may be necessary due to the sensitivity of basal growth to inhibitors are discussed. Cyanide was found to inhibit auxin-induced growth much more rapidly than acid-induced growth. These data suggest that acid growth may be dependent on respiratory metabolism but to a lesser degree than is auxin-induced growth. If the acid growth theory of auxin action is correct, it appears that there may be two steps in the growth process which are dependent on respiratory metabolism: (a) auxin-induced proton pumping which is highly sensitive to respiratory inhibitors; and (b) acid-mediated wail loosening which is moderately and perhaps indirectly sensitive to respiratory inhibitors.The rapid elongation of stem and coleoptile segments in response to auxin has been described as an active process which is very sensitive to metabolic inhibitors (14, 15). According to the acid growth hypothesis, auxin is thought to activate a metabolically driven proton pump which excretes H+ into the cell wall (5, 18). The resultant decrease in wall pH is thought to cause wall loosening which is followed by turgor-driven growth. The rapid suppressive effect (e.g. 2-10 min, see refs. 13 and 15) of respiratory inhibitors on auxin-induced growth may be caused by their ability to interfere with oxidative phosphorylation, thus deactivating the proton pump. The resulting increase in cell wall pH may be responsible for the observed cessation of wall loosening and growth.-In contrast, the rapid elongation of stem and coleoptile segments in response to external application of acid has been described as a passive process which is dependent upon turgor but insensitive to metabolic inhibitors and anaerobic conditions (14). More recently, it has been described as an enzymic wall-loosening process which is insensitive to metabolic inhibitors (18). The enhancement of growth by acid has been considered...

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Rapid Growth Inhibition of Avena Coleoptile Segments by Abscisic Acid

Cited by 41 publications

References 2 publications

The Lack of Effect of Cyclic Adenosine 3′:5′-Monophosphate on Avena Coleoptile Growth

The Lack of Effect of Cyclic Adenosine 3′:5′-Monophosphate on Avena Coleoptile Growth

Critical study of coleoptile elongation controlled by IAA and ABA I. Growth kinetics and distribution

Cyanide Inhibition of Acid-induced Growth in Avena Coleoptile Segments

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