Rapid Gout Detection Method and Kit

Abstract

Gout is a form of arthritis characterized by buildup of uric acid in synovial fluid, which causes severe swelling and can harm joints, tendons, and other tissues. It affects approximately 4% of the United States population, or approximately 8.3 million people nationwide and is therefore a topic of epidemiologic consideration due to its prevalence. Gout is typically diagnosed via polarized microscopy of arthroscopically-aspirated synovial fluid, which is a costly, time-consuming, labor-intensive, and technically complex procedure, warranting a simpler and less complex method for diagnosis. Here, we propose and validate a colorimetric method which is based on the ability of uric acid to reduce silver nitrate. We also assessed how the colorimetric change can be accelerated by changing the concentration of silver nitrate or adding different silver catalysts, as well as develop a matrix bed for improved handling and ease of use. When translated to the clinic, this diagnostic method for gout will have the potential to increase diagnostic efficiency and accelerate patient care at the bedside.

Clinical Validation of Rapid Gout Detection Method and Kit

“…As a result, we recognize the importance developing and optimizing a point-of-care gout detection method and device, which will improve the cost, time, and effort, associated with the diagnosis or rule-out of gout. Our prior work [11] formed the basis for this technique, and here, we have successfully completed the clinical validation of bedside rapid gout detection. The method we have developed is significant in that it improves upon the gold standard of polarized light microscopy for gout diagnosis; our rapid detection method is an easier, faster, and more accessible way of diagnosing gout, while still retaining the identification of uric acid crystals as the gold-standard foundation for diagnosis.…”

“…Therefore, there is no available bedside method or rapid test for the diagnosis of gout, and a point-of-care gout detection technique is necessary to improve patient care efficiency. Due to the need for a less expensive, less labor-intensive, and more efficient diagnostic method for gout, we developed a point-of-care colorimetric gout detection method, and designed a low-cost device to utilize our method [11]. Silver nitrate is reduced to silver and turns black upon addition of uric acid, which is the colorimetric basis for our rapid uric acid detection method.…”