Rapid genotype analysis of the stromelysin gene 5A/6A polymorphism

Dunleavey, L; Beyzade, Seyyare; Ye, Shu

doi:10.1016/s0021-9150(00)00443-3

Cited by 34 publications

(20 citation statements)

References 11 publications

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“…10% of samples were genotyped twice for quality control and results showed no discrepancies. PCR-RFLP methods were employed as previously published for MMP1 G-1607GG, MMP3 6A-1171 5A, MMP2 C-735T, MMP9 C-1562T, MMP7 C-153T and MMP7 A-181G [12,14,17,22].…”

Section: Methodsmentioning

confidence: 99%

Single nucleotide polymorphisms in matrix metalloproteinase genes and lung cancer chemotherapy response and prognosis

Scherf

Dally²,

Müller³

et al. 2009

European Respiratory Journal

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The prognosis for lung cancer patients treated with chemotherapy is poor. Single nucleotide polymorphisms (SNPs) in matrix metalloproteinase (MMP) genes could influence treatment outcome by altering apoptotic pathways. Eight SNPs with known or suspected phenotypic effect in six genes (MMP1, MMP2, MMP3, MMP7, MMP9 and MMP12) were investigated.For 349 Caucasian patients with primary lung cancer, receiving first-line chemotherapy, three different endpoints were analysed: response after the second cycle, progression free survival (PFS) and overall survival (OS).The prognostic value of the SNPs was analysed using multiple logistic regression for all patients and histology-, stage-and treatment-specific subgroups. Hazard ratio estimates for PFS and OS were calculated using Cox regression methods.None of the investigated polymorphisms modified response significantly in the whole patient population.However, tumour stage IIIB variant allele carriers of MMP2 C-735T showed a significantly worse response. PFS was significantly prolonged in MMP1 G-1607GG variant allele carriers and OS in small cell lung cancer patients carrying the MMP12 A-82G variant allele.In conclusion, this study identified SNPs in MMP1, MMP2, MMP7 and MMP12 for further investigation as possible predictors of chemotherapy outcome in lung cancer patients.

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Section: Methodsmentioning

confidence: 99%

Single nucleotide polymorphisms in matrix metalloproteinase genes and lung cancer chemotherapy response and prognosis

Scherf

Dally²,

Müller³

et al. 2009

European Respiratory Journal

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“…The PCR primers for amplifying the MMP-3 promoter were: forward primer 5'-GATTACAGACATGGGTCACG-3' and reverse primer 5'-TTTCAATCAGGACAAGACGAAGTTT-3' (Dunleavey et al, 2000). A mismatch close to the 3'-end of the reverse primer was made to create a restriction site in the case of the 5A allele by XmnI (New England BioLabs, UK).…”

Section: Genotype Of the Mmp-3 Polymorphismmentioning

confidence: 99%

Association between matrix metalloproteinase-3 polymorphism and anterior cruciate ligament ruptures

Malila¹,

Yuktanandana²,

Saowaprut³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Anterior cruciate ligament (ACL) ruptures are considered to be the most severe joint injury in sports. However, the precise etiologies of ACL injuries are not fully understood. Recently, the gene encoding the matrix metalloproteinase-3 (MMP-3, stromelysin-1) was shown to be associated with anterior cruciate ligament ruptures. The 5A/6A polymorphism in the promoter of the MMP-3 gene affects the regulation of MMP-3 gene expression. We examined the association between polymorphism within -1612 of the MMP-3 gene and ACL rupture in an independent population. Eighty-six participants between 20 and 40 years of age with surgically diagnosed ACL ruptures and 100 healthy controls between 18 and 28 years of age without history of ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (4): 4158-4165 (2011) MMP-3 SNP in ACL ruptures 4159 ligament or tendon injuries were recruited for the study. All participants were genotyped for the MMP-3 polymorphism (-1612 5A/6A). Statistical analyses of genotype frequencies between patients and healthy controls were performed by the chi-square test. A significant difference was found between ACL rupture subgroups in terms of genotype association (5A+ (5A/5A, 5A/6A): 37.5% in contact sports vs 20% in non-contact sports; P = 0.02). In allelic association, there were significant differences (6A: 81.2% in contact sports vs 89.1% in non-contact sports, 5A: 18.8% in contact sports vs 10.9% in non-contact sports, P = 0.01). The 5A+ genotype of MMP-3 was represented in ACL ruptures in contact sport participants. We propose that this sequence variant is a specific genetic element that should be included in a multifactorial model to understand the etiologies and risk factors for ACL rupture.

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“…28 The methods used to type the MMP-1 1G/2G, MMP-3 5A/6A, MMP-9 C-1562T, and MMP-12 A-82G polymorphisms have been described previously. 17,21,29,30 Briefly, the DNA sequence containing the relevant polymorphic site was amplified by polymerase chain reaction (PCR), and the amplicon was digested with an appropriate restriction enzyme that cleaves only 1 of the 2 alleles. The digests were then subjected to gel electrophoresis and visualized by Vistra green or ethidium bromide staining.…”

Section: Determination Of Genotypesmentioning

confidence: 99%

Polymorphisms in Matrix Metalloproteinase-1, -3, -9, and -12 Genes in Relation to Subarachnoid Hemorrhage

Zhang

Dhillon

Geary

et al. 2001

Stroke

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Background and Purpose-Intracranial aneurysm, which underlies the vast majority of subarachnoid hemorrhage incidences, has a multifactorial etiology, and the importance of genetic factors is increasingly recognized. Development and rupture of intracranial aneurysms involve degradation and remodeling of the vascular wall matrix in which the matrix metalloproteinases (MMPs) play an important role. The possible impact of MMP gene polymorphisms on susceptibility to intracranial aneurysms is still controversial, with conflicting data from different reported studies. Methods-In this study we analyzed 5 different functional promoter polymorphisms in the MMP-1, MMP-3, MMP-9, and MMP-12 genes in a sample of 92 patients with aneurysmal subarachnoid hemorrhage and 158 healthy control subjects, all from southern England. Results-No significant difference was detected between the patient and control groups in genotype distribution of any of the polymorphisms studied. Conclusions-The

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Rapid genotype analysis of the stromelysin gene 5A/6A polymorphism

Cited by 34 publications

References 11 publications

Single nucleotide polymorphisms in matrix metalloproteinase genes and lung cancer chemotherapy response and prognosis

Single nucleotide polymorphisms in matrix metalloproteinase genes and lung cancer chemotherapy response and prognosis

Association between matrix metalloproteinase-3 polymorphism and anterior cruciate ligament ruptures

Polymorphisms in Matrix Metalloproteinase-1, -3, -9, and -12 Genes in Relation to Subarachnoid Hemorrhage

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