Rapid, fully automated measurement of plasma homocyst(e)ine with the Abbott IMx analyzer

Shipchandler, M. T.; Moore, Edwin G.

doi:10.1093/clinchem/41.7.991

Cited by 324 publications

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“…These methods were later substituted by radioenzyme assays, the use of gas chromatography-mass spectrometry and the more and more frequent use of protocols of precolumn derivatization, high-pressure liquid chromatography (HPLC) and uorescence detection or, alternatively, HPLC and electrochemical detection [41±45]. However, the most decisive step towards a generalization in the determination of homocysteine levels in the analysis laboratories of clinics perhaps has been the introduction of sensitive, completely automatable immunoassays [46,47]. The results obtained with these methods show good correlation with those obtained with HPLC and gas chromatography-mass spectrometry methods [47±49].…”

Section: Methods Of Measurementmentioning

confidence: 99%

Homocysteine: an emergent cardiovascular risk factor?

Medina¹,

Amores-Sánchez

2000

Eur J Clin Investigation

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Cardiovascular disease is the leading cause of mortality and disability in the western world. In the last years, the accumulation of evidence coming from both retrospective and prospective clinical studies has led to an increased interest in the potential role of mild hyperhomocysteinemia as a major, independent risk factor for cardiovascular disease. The present paper reviews the position of homocysteine in metabolism to understand the pathogenesis of hyperhomocysteinemia, as well as the clinical data pointing to its proposed role as an independent cardiovascular disease risk factor.

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Section: Methods Of Measurementmentioning

confidence: 99%

Homocysteine: an emergent cardiovascular risk factor?

Medina¹,

Amores-Sánchez

2000

Eur J Clin Investigation

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“…The reference range for this assay is 4.2-6.2%. Plasma tHcy was determined with an automated fluorescence polarization immunoassay on an Abbott IMx analyzer (Abbott Laboratories, IL, USA), with an interassay coefficient of variation <4% [37].…”

Section: Risk Factorsmentioning

confidence: 99%

Plasma homocysteine and microvascular and macrovascular complications in type 1 diabetes: a cross‐sectional nested case–control study

Soedamah‐Muthu

Chaturvedi

Teerlink

et al. 2005

Journal of Internal Medicine

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. In multivariate models, tHcy was significantly related to macroalbuminuria (OR ¼ 1.66, 95% CI: 1.24-2.24) and hypertension (OR ¼ 1.57, 95% CI: 1.19-2.07), independent of age, sex, diabetes duration, GFR, microvascular and macrovascular complications and cardiovascular risk factors. There was a significant relationship between tHcy and decreased GFR, independent of established risk factors. The relationship between tHcy and retinopathy was not independent of albuminuria or GFR. The initial positive relationship with CVD was explained by cardiovascular risk factors. Conclusion. In this large study of European type 1 diabetic subjects, increased concentrations of tHcy were independently related to macroalbuminuria, renal function and hypertension, which suggests that tHcy might play an important role in the pathogenesis of vascular complications in type 1 diabetes.

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“…Total plasma homocysteine was determined with an automated fluorescence polarisation immunoassay on an Abbott IMx analyzer (Abbott Laboratories Ltd, IL, USA) (interassay coefficient of variation, CV, 4%) [24]. We used tandem mass spectrometry for the determination of S-adenosylmethionine and S-adenosylhomocysteine in plasma, and the whole blood, as previously described (intra-assay CV, 4% for both determinations; interassay CV, 8% and 6%, respectively) [25].…”

Section: Determination Of Homocysteine S-adenosylmethionine S-adenomentioning

confidence: 99%

S‐adenosylhomocysteine and the ratio of S‐adenosylmethionine to S‐adenosylhomocysteine are not related to folate, cobalamin and vitamin B6 concentrations

Becker¹,

Smulders²,

Teerlink³

et al. 2003

Eur J Clin Investigation

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Background It is unclear whether homocysteine itself is causal in the pathogenesis of cardiovascular disease. Alternatively or additionally, the association between homocysteine and cardiovascular disease may be because of its metabolic precursor, Sadenosylhomocysteine, or of the ratio of S-adenosylmethionine to S-adenosylhomocysteine. Therefore, it is relevant to know how these moieties are interrelated, and whether, as is the case for homocysteine, they are influenced by blood levels of folate, cobalamin or vitamin B 6 .Design We cross-sectionally studied a population-based cohort of 97 Caucasian subjects aged 60 -85 years. Concentrations of homocysteine, S-adenosylhomocysteine, Sadenosylmethionine, folate, cobalamin and vitamin B6 were measured in fasting blood samples. ResultsIn multiple regression analysis, homocysteine was associated with vitamin B12 (per 50 pmol L − 1 increase of cobalamin, change in homocysteine, -0·70 mmol L − 1 ; 95% CI, − 1·30 to − 0·10 mmol L ). S-adenosylhomocysteine, S-adenosylmethionine and the ratio of S-adenosylmethionine to S-adenosylhomocysteine were not associated with serum folate, cobalamin or vitamin B6, nor with erythrocyte folate. Furthermore, plasma homocysteine showed a negative correlation with the ratio of Sadenosylmethionine to S-adenosylhomocysteine in plasma ( r = − 0·27; P < 0·01) but not in erythrocytes.Conclusions In contrast to homocysteine, the plasma concentrations of Sadenosylhomocysteine and the ratio of S-adenosylmethionine to S-adenosylhomocysteine were not associated with the folate, cobalamin and vitamin B6 concentrations in the present study. If these precursors in part explain why homocysteine is associated with cardiovascular disease, homocysteine-lowering treatment with B vitamins may be less effective than currently expected, at least in an elderly population.

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Rapid, fully automated measurement of plasma homocyst(e)ine with the Abbott IMx analyzer

Cited by 324 publications

References 0 publications

Homocysteine: an emergent cardiovascular risk factor?

Homocysteine: an emergent cardiovascular risk factor?

Plasma homocysteine and microvascular and macrovascular complications in type 1 diabetes: a cross‐sectional nested case–control study

S‐adenosylhomocysteine and the ratio of S‐adenosylmethionine to S‐adenosylhomocysteine are not related to folate, cobalamin and vitamin B6 concentrations

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