Rapid fluorimetric procedure for the analysis of Fendosal in plasma and data following oral dosing

Abstract

A simple, rapid, and sensitive procedure is described for the determination of Fendosal in plasma. After extraction of Fendosal from buffered plasma, the drug is determined by measurement of the fluorescence induced by irradiation with short-wave ultra-violet (UV) light. The complete procedure can be completed in less than 2 h; one technician can perform up to 50 assays in one working day. The limit of detection corresponds to 0.1 microgram ml-1 plasma. Drug concentration and induced fluorescence were linearly related over the concentration range 0--8 micrograms ml-1.