Rapid Flow Through Immunoassay for CRP Determination Based on Polyethylene Filters Modified with<i>ω</i>-Aminocellulose Carbamate

Elschner, Thomas; Scholz, Friedrich; Miethe, Peter; Heinze, Thomas

doi:10.1002/mabi.201400179

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…Thus, the CRP determination could be performed by a very sensitive rapid flow through immunoassay, which shows a detection limit of 5 ng CRP/ml and a detection range of 5–250 ng CRP/ml. Depending on the ω ‐aminocellulose carbamate and the coupling structure, covalent antibody immobilization could be developed and optimized …”

Section: Applicationsmentioning

confidence: 99%

Cellulose Carbonates: A Platform for Promising Biopolymer Derivatives With Multifunctional Capabilities

Elschner

Heinze

2015

Macromolecular Bioscience

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Cellulose carbonates as a platform compound open new possibilities for the design of advanced materials based on the most important renewable resource cellulose. In the present feature, the chemistry of cellulose carbonates is discussed considering own research results adequately. After a short overview about methods for activation of polysaccharides for a conversion with nucleophilic compounds in particular with amines, details about various methods for the synthesis of polysaccharide carbonates are discussed. The main issue of the feature is the synthesis and aminolysis of cellulose carbonates with low, intermediate, and high degree of substitution and the evaluation of this chemistry with respect to specific challenges. Functional cellulose carbamates, obtained from cellulose phenyl carbonate by aminolysis, show the potential use of this class of celluloses. Immunoassays and zwitterionic polymers are included as representative examples regarding properties and application of the new cellulose-based products.

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Section: Applicationsmentioning

confidence: 99%

Cellulose Carbonates: A Platform for Promising Biopolymer Derivatives With Multifunctional Capabilities

Elschner

Heinze

2015

Macromolecular Bioscience

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“…Dialysis of the dissolved polymers in DMA against water resulted in nanoparticles, their hydrodynamic diameters, zeta potentials and PDI decreasing with increasing hydrophobicity. Functionalized cellulose based materials as biocompatible supports allow a covalent antibody conjugation with high yields and antibody activity . Preliminary studies indicate that the fluorescent nanoparticles can act as a label for biomolecules in bioassays.…”

Section: Discussionmentioning

confidence: 99%

Simple Synthesis of Reactive and Nanostructure Forming Hydrophobic Amino Cellulose Derivatives

Obst

Heinze

2015

Macromol. Mater. Eng.

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Organo-soluble amino cellulose derivatives were synthesized in a one-pot synthesis by simultaneous reaction of tosyl celluloses with ethylenediamine and 4-chlorobenzylamine. The soluble products obtained form nanoparticles in water by nanoprecipitation. As shown by dynamic light scattering (DLS) and scanning electron microscopy (SEM), their hydrodynamic diameter, zetapotential, and PDI decrease with increasing hydrophobicity. Moreover, the derivative could be labeled with Fluorescein isothiocyanate (FITC) and forms fluorescent nanoparticles with an unexpected low ydrodynamic diameter, useful for the labeling of biomolecules in bioassays.Amino tosyl cellulose 2e (300 mg, 0.76 mmol) was dissolved in 12 mL DMA and FITC (5.82 mg, 0.02 mmol, ratio FITC/NH 2 ¼ 1/5) was added. The reaction was performed at room temperature for 24 h. The product was precipitated in 125 mL ethyl acetate, washed 5 times with ethyl acetate, 3 times with ethanol and dried in vacuo. Yield: 150 mg (50%). Preparation of NanoparticlesThe polymer samples (20 mg) were dissolved in 5 mL DMA and impurities were removed by centrifugation. The solution was filled in a dialysis tube (MWCO ¼ 3 500 g Á mol À1 ) and kept in a beaker filled with distilled water. Water was changed 6 times in intervals www.mme-journal.de M. Obst, T. Heinze

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“…Moreover, it was found that ω‐aminoethylcellulose carbamate obtained by the conversion of cellulose phenyl carbonate forms thin films for functional surfaces from aqueous solutions on various substrates like gold and polyethylene (PE) depending on the pH value . Based on this observation, a method for immobilization of antibodies on ω‐aminoethylcellulose‐modified PE was developed . In the present study, the synthesis of ω‐aminoethylcellulose carbamate with low DS and of mixed ω‐aminoethyl‐ p ‐aminobenzylcellulose carbamate is discussed.…”

Section: Introductionmentioning

confidence: 94%

“…[ 19 ] Based on this observation, a method for immobilization of antibodies on ω-aminoethylcellulose-modifi ed PE was developed. [ 20 ] In the present study, the synthesis of ω-aminoethylcellulose carbamate with low DS and of mixed ω-aminoethylpaminobenzylcellulose carbamate is discussed. These novel cellulose derivatives were investigated due to their biocompatibility and cytotoxicity against human HaCaT keratinocytes and antimicrobial activity in vitro.…”

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confidence: 98%

Synthesis of Novel Cellulose Carbamates Possessing Terminal Amino Groups and Their Bioactivity

Ganske

Wiegand

Hipler

et al. 2015

Macromolecular Bioscience

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Cellulose phenyl carbonates are an excellent platform to synthesize a broad variety of soluble and functional cellulose carbamates. In this study, the synthesis of cellulose carbamates with terminal amino groups, namely ω-aminoethylcellulose- and ω-aminoethyl-p-aminobenzyl-cellulose carbamate, is discussed. The products are well soluble and their structures can be clearly described by NMR spectroscopy. The cellulose carbamates exhibit a bactericide and fungicide activity in vitro. The ω-aminoethylcellulose carbamate possesses a strong activity against Candida albicans and Staphylococcus aureus (IC50 of 0.02 mg mL(-1) and 0.05 mg mL(-1)). The antimicrobial activity and cytotoxicity can be improved by p-amino-benzylamine (ABA) as an additional substituent. The mixed cellulose carbamate exhibits a high biocompatibility (LC50 of 3.18 mg mL(-1)) and forms films on cotton and PES, which exhibit a strong activity against S. aureus and Klebsiella pneumoniae.

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Rapid Flow Through Immunoassay for CRP Determination Based on Polyethylene Filters Modified withω-Aminocellulose Carbamate

Cited by 10 publications

References 33 publications

Cellulose Carbonates: A Platform for Promising Biopolymer Derivatives With Multifunctional Capabilities

Cellulose Carbonates: A Platform for Promising Biopolymer Derivatives With Multifunctional Capabilities

Simple Synthesis of Reactive and Nanostructure Forming Hydrophobic Amino Cellulose Derivatives

Synthesis of Novel Cellulose Carbamates Possessing Terminal Amino Groups and Their Bioactivity

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