Rapid fabrication of keratin–hydroxyapatite hybrid sponges toward osteoblast cultivation and differentiation

Tachibana, Akira; Kaneko, Sumika; Tanabe, Toshizumi; Yamauchi, Kiyoshi

doi:10.1016/j.biomaterials.2004.02.032

Cited by 178 publications

(124 citation statements)

References 17 publications

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“…Therefore, the chemical composition, controlled biodegradability and biologically functional properties are all important attributes for scaffolds used in TE [1][2][3] . Calcium phosphates such as hydroxyapatite (HA; Ca 10 (PO 4 ) 6 (OH) 2 ) have shown good biocompatibility and osteoconductivity 4,5 , although their mechanical strength limits their use to non-load bearing applications 6,7 . HA synthesis by wet precipitation is largely used and can be obtained by different routes.…”

Section: Introductionmentioning

confidence: 99%

“…Those reactions allow the improvement of different structural, mechanical and physico-chemical properties 8 . The use of Col fibrils dissociated in a solution seems to provide an effective interaction between Col molecules and the mineral phase precipitated in comparison with other composite routes 1,2,5,[7][8][9] . The cross-linking process induces a significant change in the Col structure from the nanoscale to microscale range 8,10,11 and it is usually necessary in order to reduce its immunogenicity and control material biodegradation 11 .…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

2011

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Hydroxyapatite-collagen (HA/Col) composites are potential scaffolds for bone tissue engineering. In this work, three-dimensional (3-D) HA/Col (50/50 wt. (%)) scaffolds were synthesized using a selfassembly method and cross-linked with a 0.125% glutaraldehyde solution. Scaffolds were evaluated in vitro by cytotoxicity testing using MC3T3 cells; proliferation and differentiation were studied using STRO-1A human stromal cells for up to 21 days. Morphological and histological examinations showed a fibrous structure with a good distribution and homogeneous HA particles distribution. By thermogravimetric analysis, a ratio of 1.2 between inorganic and organic phase was found. The scaffolds presented no cytotoxicity when evaluated using three different parameters of cell survival and integrity: 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT), Neutral Red (NR) and Crystal Violet Dye Elution (CVDE). STRO-1A cells were found to adhere, proliferate and differentiate on the 3-D scaffold, but limited cell penetration was observed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

2011

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“…Hydroxyapatite can be made porous/dense, resorbable/solid and it can be produced in powders/blocks 1 . Bone is a complex material composed of proteins, mainly collagen, with hydroxyapatite, therefore, now many investigations are focused on the hybrid biomaterials of hydroxyapatite with proteins and other synthetic polymers 2 . Biomaterials may have low, medium or high potential risk to human safety, depending on the type and extent of patient contact.…”

Section: Introductionmentioning

confidence: 99%

Comparison the Cytotoxicity of Hydroxyapatite Measured by Direct Cell Counting and MTT Test in Murine Fibroblast Nih-3t3 Cells

Theiszová¹,

Jantová²,

Dragúňová³

et al. 2005

BIOMED PAP

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The worldwide growing interest to biomaterials over the last years results from their irreplaceable role in medical clinic. Hydroxyapatite is used in bone reconstruction because of its similar chemical structure compared to the inorganic composition of human bone and it is basic building component of many newly prepared biomaterials. In this study, we evaluated cytotoxic/antiproliferative activity of hydroxyapatite extract using murine fibroblast cell line NIH-3T3 and two in vitro different cytotoxic assays: growth inhibition assay and MTT assay. Hydroxyapatite extract after 72 h of incubation manifested the significant in vitro cytotoxic/antiproliferative effect only at the highest concentration tested (100 %). The antiproliferative effect of hydroxyapatite extract at the other concentrations tested (75 %, 50 %, 25 %, 10 %, 5 % and 1 %) was directly proportional to the concentration and the time of influence. The inhibition of cell proliferation was 86.8 -0 %. The sensitivity of cell growth inhibition assay (direct counting of viable cells) to the extract influence was higher than that of MTT test.

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“…Certain keratin materials have been shown to be mitogenic and chemotactic for a variety of cell types, and to mediate changes in gene expression consistent with the promotion of wound healing. Moreover, other recent works (Tachinaba et al, 2002;Tachinaba et al, 2005;Hamasaki et al, 2008) have highlighted the excellent cell adhesion onto wool keratin sponges produced by lyophilisation from aqueous solution. These results show that keratin is a useful biomaterial for scaffolds for cell cultivation.…”

Section: Keratin/peo Blend Nanofibresmentioning

confidence: 99%

Keratin-based Nanofibres

Tonin¹,

Aluigi²,

Varesano³

et al. 2010

Nanofibers

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Rapid fabrication of keratin–hydroxyapatite hybrid sponges toward osteoblast cultivation and differentiation

Cited by 178 publications

References 17 publications

In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

Comparison the Cytotoxicity of Hydroxyapatite Measured by Direct Cell Counting and MTT Test in Murine Fibroblast Nih-3t3 Cells

Keratin-based Nanofibres

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