Rapid extraction of bacterial genomic DNA with guanidium thiocyanate

Pitcher, D.G.; Saunders, Nicholas A.; Owen, Robert J.

doi:10.1111/j.1472-765x.1989.tb00262.x

Cited by 1,996 publications

(1,331 citation statements)

References 15 publications

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“…Chromosomal DNA was isolated by using the guanidium isothiocyanate method [22] with the exception that phenol-chloroform (50: 50) was used for DNA extraction instead of chloroform. DNA (5 jag) was digested with Sma I ribonuclease (Boehringer Mannheim GmbH Mannheim, Germany).…”

Section: Identification To the Genos3pecies Levelmentioning

confidence: 99%

Distribution ofAeromonasphenospecies and genospecies among strains isolated from water, foods or from human clinical samples

Hänninen

Siitonen

1995

Epidemiol. Infect.

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SUMMARYA total of 332 Aeromonas spp. originating from drinking water (n = 75), fresh water (n = 57), chicken and ground beef (107), human faecal samples in association with travelling (n = 49), human faecal samples not associated with travelling (n = 38), and six strains from human blood cultures were studied by phenotypic methods and by using analysis of ribopatterns as a molecular method for the identification of the 13 known hybridization groups (HGs). Also included were the reference strains of each HG. A. hydrophila HG 1, A. caviae HG 4 and A. veronii biotype sobria HG 8/10 were the most important genospecies identified in human faecal samples. A. hydrophila HG 2 and A. media HG 5B predominated in drinking water and A. hydrophila HG 2 and HG 3, A. media HG 5A and HG 5B predominated in fresh water. In drinking water only one isolate was A. hydrophila HG 1 and two isolates were A. caviae HG 4. Clinically important Aeromonas spp. HG 1 (A. hydrophila), HG 4 (A. caviae) and HG 8/10 (A. veronii biotype sobria) were common in chicken and ground beef. In contrast to the drinking water samples, HG 5A was common in chicken and ground beef samples. Atypical, unidentified isolates were most often found in fresh water samples (12/57 strains). Although water has been suspected of being an important source of human aeromonas infections, clinically important HGs were found to be in the minority among Aeromonas spp. identified in drinking water or fresh water. The distribution of Aeromonas spp. HGs among drinking water, chicken and ground beef samples was also different, suggesting that contamination of meat or chicken may not originate from water.

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Section: Identification To the Genos3pecies Levelmentioning

confidence: 99%

Distribution ofAeromonasphenospecies and genospecies among strains isolated from water, foods or from human clinical samples

Hänninen

Siitonen

1995

Epidemiol. Infect.

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“…Total genomic DNA from bacterial cells was extracted and purified according to Pitcher et al [16]. Enzyme reactions and PCR were conducted in sterilized Mili Q water.…”

Section: Preparation Of Samples For Aflp Fingerprintingmentioning

confidence: 99%

Genetic Diversity and Pathogenicity of Xanthomonas axonopodis Strains Inducing Citrus Canker Disease in Iran and South Korea

Khodakaramian

Swings²

2011

Indian J Microbiol

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“…Total genomic DNA was extracted from the 10 ml culture by the method of Pitcher et al [21] with minor modifications. The lysozyme step for Gram-positive bacteria was incorporated and sarkosyl was omitted from the guanidium thiocyanate step.…”

Section: Faflpmentioning

confidence: 99%

Use of AFLP and PFGE to discriminate between Salmonella enterica serovar Typhimurium DT126 isolates from separate food-related outbreaks in Australia

Ross

Heuzenroeder

2005

Epidemiol. Infect.

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SUMMARYIn 2001 Salmonella enterica serovar Typhimurium definitive phage-type (DT) 126 was isolated at higher frequency in Australia compared to other S. Typhimurium phage types and in comparison to previous years. Associated with this increase was the implication of this phage type in a number of food-related outbreaks. We compared fluorescent amplified fragment length polymorphism (FAFLP) to pulsed-field gel electrophoresis (PFGE), the current ' gold standard ' for molecular typing of Salmonella for the discrimination between outbreak-associated isolates and epidemiologically unrelated DT126 strains. FAFLP showed a greater ability to discriminate between isolates than PFGE, with 16 groups of clusters or individual isolates with <90 % similarity to each other compared to three groups as determined by PFGE. Both methods were able to discriminate between isolates from two separate outbreaks in South Australia and isolates associated with an outbreak at a restaurant in New South Wales. The resolving power of both methods was not sufficient to separate all epidemiologically unrelated DT126 isolates from the outbreak isolates. We conclude that amplified fragment length polymorphism is a useful tool to assist in the discrimination of S. Typhimurium DT126 isolates.

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Rapid extraction of bacterial genomic DNA with guanidium thiocyanate

Cited by 1,996 publications

References 15 publications

Distribution ofAeromonasphenospecies and genospecies among strains isolated from water, foods or from human clinical samples

Distribution ofAeromonasphenospecies and genospecies among strains isolated from water, foods or from human clinical samples

Genetic Diversity and Pathogenicity of Xanthomonas axonopodis Strains Inducing Citrus Canker Disease in Iran and South Korea

Use of AFLP and PFGE to discriminate between Salmonella enterica serovar Typhimurium DT126 isolates from separate food-related outbreaks in Australia

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