Rapid Extraction Method with Pronase B for Grouping Beta-Hemolytic Streptococci

Ederer, Grace Mary; Herrmann, Mary M.; Bruce, Robert A.; Matsen, John M.; Chapman, S. Stephen

doi:10.1128/aem.23.2.285-288.1972

Cited by 26 publications

(11 citation statements)

References 3 publications

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“…A possible explanation for these negative responses with the Streptex kit may be associated with the vagueness of the explanation of its package insert concerning the density of the bacterial suspension required for the pronase extraction. Extraction enzymes such as Pronase B have been shown to be effective for the preparation of group-specific streptococcal antigens (5). However, this procedure re-…”

Section: Discussionmentioning

confidence: 99%

Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci

Slifkin¹,

Pouchet-Melvin²

1980

J Clin Microbiol

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Three beta-streptococci serogrouping kits, Phadebact, SeroSTAT, and Streptex, were evaluated as to their sensitivity, accuracy, and suitability as methods for serogrouping streptococci in a clinical microbiology laboratory. The majority of the primary isolates examined by the various methods associated with each of the three kits were correctly identified. The Streptex direct mixed-culture procedure was more often associated with the observation of cross-reactivity than with the direct procedures of the other two kits which did not employ mixed growth cultures. Furthermore, the Streptex kit was associated with more falsenegative responses than those determined by the other two kits under evaluation. These results appeared to be due to the relatively poor sensitivity of the Streptex grouping reagents. The Streptex test procedures required more labor than the other kit procedures, requiring a 1-h enzymatic extraction step for the release of the group antigens. The SeroSTAT kit provided only a direct procedure and, thus, is limited in its application. The Phadebact procedures were the most versatile by providing not only a direct and a 24-h grouping procedure, but also by including a 4-h method that may be employed as required by the clinical microbiologist.

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Section: Discussionmentioning

confidence: 99%

Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci

Slifkin¹,

Pouchet-Melvin²

1980

J Clin Microbiol

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“…The group determination of streptococci is most commonly performed by using various precipitation techniques with group-specific antisera and extracts of streptococcal group antigens (3,8,14,16,18,22) and also by immunofluorescent methods (7,17). Direct agglutination of untreated streptococci by group-specific antisera does not work regularly for two reasons: first, group-specific antigens are usually not exposed, and secondly, many strains show saline agglutinability (15,19).…”

Section: Kronvall In Press)mentioning

confidence: 99%

New Method for the Serological Grouping of Streptococci with Specific Antibodies Adsorbed to Protein A-Containing Staphylococci

et al. 1973

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A coagglutinating reagent has recently been developed and utilized in capsule typing of pneumococci. The reagent consists of stabilized staphylococci coated with specific antibody via the gamma globulin Fc-protein A reaction thereby orienting the antibody molecules with the antigen-reactive Fab parts outwards. Reagent staphylococci coated with antibodies directed against streptococcal group-specific antigens have been used in the present investigations. Overnight cultures of streptococci were treated with trypsin and then directly tested for coagglutination of selected reagent staphylococci on glass slides. In all, 179 strains of streptococci were analyzed by the method described. The results were compared with grouping by using Lancefield extracts in counter-immunoelectrophoresis and were shown to agree completely. The coagglutination method described proved to be accurate, rapid, and simple.

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“…Over the years, a number of reports have evaluated the bacitracin sensitivity of various streptococcal groups. Those which evaluated the bacitracin results for each group either used homemade strips containing more than 0.02 gm of bacitracin (5), a 1: 5,000 dilution of the drug (8), or the number of non-group A strains studied were small (3,4,9). The reported false-positive reactions in the latter studies were extremely high.…”

mentioning

confidence: 99%

Distribution of Streptococcal Groups in Clinical Specimens with Evaluation of Bacitracin Screening

Pollock¹,

Dahlgren²

1974

Applied Microbiology

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During a 2-year period, 4,968 strains of beta-hemolytic streptococci were examined for the clinical source distribution and bacitracin sensitivity of each group. In the upper respiratory tract, groups A (51.7%) and C (20.4%) accounted for most of the isolates, and in wounds and exudates group A (79.1%) made up most of the isolates. Group B (71.2%) was the major component of isolates from the genitourinary tract and, while composing 29.3% of the lower respiratory tract isolates, competed with group A (18.8%) and the nongroupables (22.8%) for supremacy. Bacitracin screening showed that 0.5% of group A streptococci were resistant, and sensitive non-group A isolates were group B (2.6%), group C (6.0%), group G (8.0%), and the nongroupables (2.2%). It was found that those groups which were most predominant in wounds and the upper respiratory tract gave the highest rate of false positives with bacitracin, whereas the predominant group of the genitourinary tract gave the lowest rate of false positives.

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Rapid Extraction Method with Pronase B for Grouping Beta-Hemolytic Streptococci

Cited by 26 publications

References 3 publications

Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci

Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci

New Method for the Serological Grouping of Streptococci with Specific Antibodies Adsorbed to Protein A-Containing Staphylococci

Distribution of Streptococcal Groups in Clinical Specimens with Evaluation of Bacitracin Screening

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