Rapid extraction and analysis of oxidative stress and DNA damage biomarker 8-hydroxy-2′-deoxyguanosine (8-OHdG) in urine: Application to a study with pregnant women

Bláhová, Lucie; Janoš, Tomáš; Mustieles, Vicente; Rodríguez-Carrillo, Andrea; Fernández, Mariana F.; Bláha, Luděk

doi:10.1016/j.ijheh.2023.114175

Cited by 7 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8-OHdG served as a commonly utilized biomarker to detect oxidative DNA damage resulting from oxidative stress (Bláhová et al, 2023). Relative to healthy rats, elevated expression levels of 8-OHdG were observed within the colon mucosa of CC-afflicted mice, as illustrated in Figure 6.…”

Section: Oxidative Stressmentioning

confidence: 99%

The Impact of Atmospheric Cadmium Exposure on Colon Cancer and the Invasiveness of Stents within the Cancerous Colon

Zhang,

Li,

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: Inhalation exposure to carcinogenic metals like cadmium (Cd) is a significant global health concern linked to various cancers. However, understanding their precise carcinogenic mechanism remains elusive. Methods: Using the colon cancer (CC) rat model and implanted intestinal stent, exposed them to atomized oxygen and nitrogen (O2/N2) gas containing Cd. Results: Atmospheric Cd intensified inflammation in CC cells, heightening nicotinamide adenine dinucleotide phosphate 1 (NOX1) activity, generating increased reactive oxygen species (ROS). This escalated ROS production triggered abnormal Wnt protein secretion, activating the Wnt/β-catenin pathway, stimulating CC cell proliferation. No discernible body weight effect was seen in CC mice, possibly due to later-stage tumor weight gain, masking rat changes. Cd facilitated colon tumor restructuring and cell migration later. Intestinal stents inhibited superoxide dismutase 1 (SOD1) in CC mice's tumors, with no clear effects on NOX1, SOD2, and catalase (CAT) enzymes. Elevated ROS levels didn't substantially impact Wnt/β-catenin and might slow its imbalance. Stent implantation eased inflammation, reducing CC cell proliferation but induced discomfort in rats, reducing food intake and weight. Conclusion: Cd might partially foster CC tumorigenesis via the ROS-mediated Wnt/β-catenin pathway. The effect of Cd on the invasive effect of intestinal stents in cancerous colon was not significant.

show abstract

Section: Oxidative Stressmentioning

confidence: 99%

The Impact of Atmospheric Cadmium Exposure on Colon Cancer and the Invasiveness of Stents within the Cancerous Colon

Zhang,

Li,

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…MMP-9 primarily participates in the activation and migration of inflammatory cells [ 34 ]. 8-OHdG serves as a commonly utilized biomarker to detect oxidative DNA damage resulting from oxidative stress [ 35 ]. However, the impact of atmospheric Cd on the invasiveness of stents in the cancerous colon remains largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Atmospheric Cadmium Exposure on Colon Cancer and the Invasiveness of Intestinal Stents in the Cancerous Colon

Zhang,

Li,

et al. 2024

Toxics

View full text Add to dashboard Cite

Background: Inhalation exposure to carcinogenic metals such as cadmium (Cd) is a significant global health concern linked to various cancers. However, the precise carcinogenic mechanism underlying inhalation exposure remains elusive. Methods: In this study, CT26 mouse colon cancer (CC) cells were implanted into BALB/c mice to establish CC mouse models. Some of the CC mice were implanted with intestinal stents. The mice were exposed to atomized oxygen and nitrogen (O2/N2) gas containing Cd. Results: Atmospheric Cd intensified inflammation in CC cells and heightened Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase 1 (NOX1) activity, which is an indirect measurement of increased reactive oxygen species (ROS) production. This escalated ROS production triggered abnormal Wnt protein secretion, activated the Wnt/β-catenin signaling pathway, and stimulated CC cell proliferation. No discernible body weight effect was seen in the CC mice, possibly due to the later-stage tumor weight gain, which masked the changes in body weight. Cd facilitated colon tumor restructuring and cell migration at the later stage. The implantation of intestinal stents inhibited the expression of Superoxide Dismutase 1 (SOD1) in the colon tumors of the CC mice, with no evident effects on the expression levels of NOX1, SOD2, and Catalase (CAT) enzymes. Elevated ROS levels, indirectly reflected by enzyme activity, did not substantially impact the Wnt/β-catenin signaling pathway and even contributed to slowing its imbalance. Stent implantation eased the inflammation occurring in colon tumors by reducing CC cell proliferation but it induced discomfort in the mice, leading to a reduction in food intake and weight. Conclusions: Cd partially fosters CC tumorigenesis via the ROS-mediated Wnt/β-catenin signaling pathway. The effect of Cd on the invasive effect of intestinal stents in the cancerous colon is not significant.

show abstract

“…Hydroxyl radicals may attack cellular membranes, proteins, nucleic acids, nuclear DNA, mitochondrial DNA, and RNA, during which 8-hydroxydeoxyguanosine (8-OHdG) forms abundantly and stablely [ 14 ]. At present, the most commonly used and promising biomarkers 8-hydroxydeoxyguanosine (8-OHdG) for oxidative stress have been investigated in medicine and toxicology [ 15 , 16 ]. It has been proposed that the content of 8-OHdG is the most studied biomarker of DNA damage-induced diseases, including cancer [ 17 , 18 , 19 ], neurodegenerative disorders [ 20 , 21 ], diabetes [ 22 , 23 ], cardiovascular [ 24 , 25 ], and infectious diseases [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Sensing System for 8-OHdG Detection Based on Plasma Coupled Electrochemistry by Transparent ITO/AuNTAs/PtNPs Electrode

Bai

2023

Biosensors

View full text Add to dashboard Cite

8-Hydroxydeoxyguanosine (8-OHdG) is the most widely used oxidative stress biomarker of the free radical-induced oxidative damage product of DNA, which may allow a premature assessment of various diseases. This paper designs a label-free, portable biosensor device to directly detect 8-OHdG by plasma-coupled electrochemistry on a transparent and conductive indium tin oxide (ITO) electrode. We reported a flexible printed ITO electrode made from particle-free silver and carbon inks. After inkjet printing, the working electrode was sequentially assembled by gold nanotriangles (AuNTAs) and platinum nanoparticles (PtNPs). This nanomaterial-modified portable biosensor showed excellent electrochemical performance for 8-OHdG detection from 10 μg/mL to 100 μg/mL by our self-developed constant voltage source integrated circuit system. This work demonstrated a portable biosensor for simultaneously integrating nanostructure, electroconductivity, and biocompatibility to construct advanced biosensors for oxidative damage biomarkers. The proposed nanomaterial-modified ITO-based electrochemical portable device was a potential biosensor to approach 8-OHdG point-of-care testing (POCT) in various biological fluid samples, such as saliva and urine samples.

show abstract

Rapid extraction and analysis of oxidative stress and DNA damage biomarker 8-hydroxy-2′-deoxyguanosine (8-OHdG) in urine: Application to a study with pregnant women

Cited by 7 publications

References 44 publications

The Impact of Atmospheric Cadmium Exposure on Colon Cancer and the Invasiveness of Stents within the Cancerous Colon

The Impact of Atmospheric Cadmium Exposure on Colon Cancer and the Invasiveness of Stents within the Cancerous Colon

The Impact of Atmospheric Cadmium Exposure on Colon Cancer and the Invasiveness of Intestinal Stents in the Cancerous Colon

Oxidative Stress Sensing System for 8-OHdG Detection Based on Plasma Coupled Electrochemistry by Transparent ITO/AuNTAs/PtNPs Electrode

Contact Info

Product

Resources

About