Rapid expression of RASD1 is regulated by estrogen receptor-dependent intracellular signaling pathway in the mouse uterus

Kim, Hye Ryun; Cho, Kil-Sang; Kim, Eunhye; Lee, Ok-Hee; Yoon, Hyung Ku; Lee, Sang-Ho; Moon, Sohyeon; Park, Miseon; Hong, Kwonho; Na, Younghwa; Shin, Jumi A.; Kwon, Hwang; Song, Haengseok; Choi, Dong Hee; Choi, Yun‐Sang

doi:10.1016/j.mce.2017.02.006

Cited by 11 publications

(21 citation statements)

References 27 publications

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“…We checked the expression of dexamethasone-induced RAS-related protein 1 (Rasd1) and lactoferin (Lf ) to confirm estrogen response in ovariectomized mice. Rasd1 and Lf were known as either early-or late-response genes, respectively [2]. Progesterone treatment didn't affect the expression of Stk4 in ovariectomized mice ( Figure 2C-D).…”

Section: Hormonal Regulation Of Stk3/4 Expression In Mouse Uterusmentioning

confidence: 93%

“…In the previous study, we proved that estrogen signal in uterine epithelium was mediated by p38 mitogen-activated protein (MAP) kinase pathway [2]. Therefore, we examined the relationship between Hippo signaling and non-genomic estrogen signaling pathway.…”

Section: Regulation Of Downstream Factors In the Hippo Signaling Pathmentioning

confidence: 97%

“…BIRC5 NM_001012271.1 (1) Fwd; 5 -AGGACCACCGCATCTCTACAT-3 (2) Rev; 5 -AAGTCTGGCTCGTTCTCAGTG-3 118 CTGF NM_001901.2 (1) Fwd; 5 -TCTTCGGTGGTACGGTGTAC-3 (2) Rev; 5 -TGTCTTCCAGTCGGTAAGCC-3 249…”

Section: Klf5mentioning

confidence: 99%

“…NM_005225.2 (1) Fwd; 5 -CTTCGTAGCATTGCAGACCC-3 (2) Rev; 5 -AAAACATCGATCGGGCCTTG-3 137 CTNNB1 NM_001904.3 (1) Fwd; 5 -CTTACACCCACCATCCCACT-3 (2) Rev; 5 -CCTCCACAAATTGCTGCTGT-3 197 GAPDH AY340484.1 (1) Fwd; 5 -ATGGGGAAGGTGAAGGTCG-3 (2) Rev; 5 -GGGTCATTGATGGCAACAAT-3 107 (1) Fwd; forward primer, (2) Rev; reverse primer.…”

Section: E2fmentioning

confidence: 99%

“…The uterus is one of most important reproductive organs for successful pregnancy on mammals [1,2]. Unlike other tissues, the uterus undergoes dynamic cyclic changes in dependence on the estrous cycle.…”

Section: Introductionmentioning

confidence: 99%

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STK3/4 Expression Is Regulated in Uterine Endometrial Cells during the Estrous Cycle

Moon

Lee

et al. 2019

Cells

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The uterus is dynamically regulated in response to various signaling triggered by hormones during the estrous cycle. The Hippo signaling pathway is known as an important signaling for regulating cellular processes during development by balancing between cell growth and apoptosis. Serine/threonine protein kinase 3/4 (STK3/4) is a key component of the Hippo signaling network. However, the regulation of STK3/4-Hippo signaling in the uterus is little known. In this study, we investigated the regulation and expression of STK3/4 in the uterine endometrium during the estrous cycle. STK3/4 expression was dynamically regulated in the uterus during the estrous cycle. STK3/4 protein expression was gradually increased from the diestrus stage and reached the highest in the estrus stage. STK3/4 was exclusively localized in the luminal and glandular epithelial cells of the uterus, and phosphorylated STK3/4 was also increased at the estrus stage. Moreover, the increase of STK3/4 expression in uteri was induced by administration of estradiol, but not by progesterone injection in ovariectomized mice. Pretreatment with an estrogen receptor antagonist ICI 182,780 reduced estrogen-induced STK3/4 expression and its phosphorylation. The estrogen-induced STK3/4 expression was related to the increase in phosphorylation of downstream targets including LATS1/2 and YAP. These findings suggest that STK3/4-Hippo signaling acts a novel signaling pathway in the uterine epithelium and STK3/4-Hippo is one of key molecules for connecting between the estrogen downstream signaling pathway and the Hippo signaling pathway leading to regulate dynamic uterine epithelium during the estrous cycle.

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Section: Hormonal Regulation Of Stk3/4 Expression In Mouse Uterusmentioning

confidence: 93%

Section: Regulation Of Downstream Factors In the Hippo Signaling Pathmentioning

confidence: 97%

“…BIRC5 NM_001012271.1 (1) Fwd; 5 -AGGACCACCGCATCTCTACAT-3 (2) Rev; 5 -AAGTCTGGCTCGTTCTCAGTG-3 118 CTGF NM_001901.2 (1) Fwd; 5 -TCTTCGGTGGTACGGTGTAC-3 (2) Rev; 5 -TGTCTTCCAGTCGGTAAGCC-3 249…”

Section: Klf5mentioning

confidence: 99%

Section: E2fmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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STK3/4 Expression Is Regulated in Uterine Endometrial Cells during the Estrous Cycle

Moon

Lee

et al. 2019

Cells

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RAS-related protein 1: an estrogen-responsive gene involved in development and molting-mediated regeneration of the female reproductive tract in chickens

Jeong

Bae

Lim

et al. 2018

Animal

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It is important to identify molecular candidates involved in morphological and functional changes in the female reproductive system. We have discovered several candidate genes that were significantly altered in chick oviducts by exogenous estrogen and those candidates included dexamethasone (DEX)-induced RAS-related protein 1 (RASD1). RAS-related protein 1, a member of the Ras family of monomeric G proteins, is involved in various cellular processes including cell growth, proliferation and differentiation, as well as a cell-signaling protein regulating hormonal actions. Although the RASD1 gene was first identified as a DEX (a corticosteroid) inducible gene, there is evidence that it is also an estrogen-responsive gene. However, hormone-mediated expression and biological functions of RASD1 in the avian female reproductive system are poorly understood. Therefore, we tested the hypothesis that RASD1 may be involved in the development and remodeling of the chicken reproductive system as an estrogen-responsive gene. Here we demonstrate differential expression of RASD1 gene and candidate microRNAs (miRNAs) targeting chicken RASD1 transcripts in chicken oviducts in response to diesthylstilbestrol (DES, a synthetic non-steroidal estrogen) and the estrogen-mediated molting process. Result of the present study indicated that expression of RASD1 messenger RNA (mRNA) increased in the developing oviducts of chicks treated with DES, particularly in the glandular (GE) and luminal (LE) epithelia of the magnum and the shell gland. Also, during induced molting by zinc feeding, RASD1 expression changed in concert with changes in concentrations of estrogen in blood of laying hens. Our results revealed that expression of RASD1 mRNA decreased as the oviduct regressed and then increased as the oviduct underwent re-growth and recrudescence in hens. Furthermore, RASD1 mRNA was expressed predominantly in GE and LE of the oviduct of laying hens during regeneration of the oviduct after induced molting, but not during the period of regression of the oviduct during molting. In addition, the relative expression of candidate miRNAs (miR-30a-5p, miR-30b-5p, miR-30c-5p and miR-30d) regulating RASD1 transcripts changed in response to estrogen stimulation of chick oviducts. These results indicate that transcription of the RASD1 gene and miRNAs regulating post-transcriptional aspects of expression of RASD1 are modulated by estrogen which is critical for growth, development, remodeling and maintenance of function of the chicken oviduct.

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A personalized mRNA signature for predicting hypertrophic cardiomyopathy applying machine learning methods

Gu,

Zhao,

Ben

et al. 2024

Sci Rep

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Rapid expression of RASD1 is regulated by estrogen receptor-dependent intracellular signaling pathway in the mouse uterus

Cited by 11 publications

References 27 publications

STK3/4 Expression Is Regulated in Uterine Endometrial Cells during the Estrous Cycle

STK3/4 Expression Is Regulated in Uterine Endometrial Cells during the Estrous Cycle

RAS-related protein 1: an estrogen-responsive gene involved in development and molting-mediated regeneration of the female reproductive tract in chickens

A personalized mRNA signature for predicting hypertrophic cardiomyopathy applying machine learning methods

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