Rapid ex vivo expansion of human umbilical cord hematopoietic progenitors using a novel culture system

Kawada, Hiroshi; Ando, Kiyoshi; Tsuji, Takashi; Shimakura, Yasuhito; Nakamura, Yoshihiko; Chargui, Jamel; Hagihara, Masao; Itagaki, Hiroyuki; Shimizu, Takashi; Inokuchi, Sadaki; Kato, Shingo; Hotta, Tomomitsu

doi:10.1016/s0301-472x(99)00012-0

Cited by 117 publications

(69 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Colony-forming units in culture (CFU-C) and mixed colonies (CFU-Mix) were enhanced by 30-fold and 10-fold, respectively. The severe-combined immunodeficient (SCID) mouse-repopulating cell (SRC) assay confirmed extensive ability of the expanded PPC to reconstitute long-term hematopoiesis [6].…”

Section: Cd38mentioning

confidence: 87%

Murine Stromal Cell Line HESS‐5 Maintains Reconstituting Ability of Ex Vivo‐Generated Hematopoietic Stem Cells from Human Bone Marrow and Cytokine‐Mobilized Peripheral Blood

et al. 2000

Self Cite

View full text Add to dashboard Cite

Human bone marrow (BM) or mobilized peripheral blood (mPB) CD34+ cells have been shown to loose their stem cell quality during culture period more easily than those from cord blood (CB). We previously reported that human umbilical CB stem cells could effectively be expanded in the presence of human recombinant cytokines and a newly established murine bone marrow stromal cell line HESS-5. In this study we assessed the efficacy of this xenogeneic coculture system using human BM and mPB CD34 + cells as materials. We measured the generation of CD34

show abstract

Section: Cd38mentioning

confidence: 87%

Murine Stromal Cell Line HESS‐5 Maintains Reconstituting Ability of Ex Vivo‐Generated Hematopoietic Stem Cells from Human Bone Marrow and Cytokine‐Mobilized Peripheral Blood

et al. 2000

Self Cite

View full text Add to dashboard Cite

show abstract

“…Up to date, most research on ex vivo expansion has explored different combinations of cytokines or chemokines, culture medium ingredients, and recently the role of stromal support. [1][2][3][4][5][6][7] Flt3-ligand (FL), thrombopoietin (TPO), stem cell factor (SCF) and interleukin-6 (IL6) are known to be early acting cytokines and have been reported to support primitive HSPC proliferation with maintenance of long-term repopulating ability. [8][9][10][11] The important effects of these cytokines on early HSPC have led to the development of in vitro culture systems in order to expand these cells.…”

Section: Introductionmentioning

confidence: 99%

Stromal support augments extended long-term ex vivo expansion of hemopoietic progenitor cells

et al. 2001

View full text Add to dashboard Cite

Current technology to numerically expand hemopoietic stem/progenitor cells (HSPC) ex vivo within 1 to 2 weeks is insufficient to warrant significant gain in reconstitution time following their transplantation. In order to more stringently test the parameters affecting HSPC expansion, we followed ex vivo cultures of CD34 + -selected umbilical cord blood (UCB) HSPC for up to 10 weeks and investigated the effects of stromal support and cytokine addition.

show abstract

“…Some of these have been shown to be capable of supporting maintenance or expansion of transplantable stem cells. [13][14][15][16][18][19][20] This discrepancy in the stromal cell dependency of ex vivo stem cell expansion may be resolved by detailed characterization of the exact roles of stromal cells. The mechanisms responsible for the supportive effects of MS-5 cell are not fully understood.…”

Section: Discussionmentioning

confidence: 99%

“…[8][9][10][11] On the other hand, however, the importance of stromal cells in the maintenance and expansion of primitive progenitors including transplantable stem cells have been repeatedly demonstrated. [12][13][14][15][16][17][18][19][20] A number of stromal cell lines have been reported to support the proliferation of human primitive progenitors. MS-5 is one such cell line, derived from noninfected Dexter-type murine long-term marrow culture, which supports CFU-S maintenance 21 and acts synergistically with human growth factors to stimulate the formation of blast colonies and macroscopic colonies from CD34 + CD38 Ϫ primitive progenitors in short-term methylcellulose assays.…”

mentioning

confidence: 99%

Stromal cell-dependent ex vivo expansion of human cord blood progenitors and augmentation of transplantable stem cell activity

Kanai

Hirayama²,

Miki³

et al. 2000

Bone Marrow Transplant

View full text Add to dashboard Cite

Summary:In The remarkable proliferation and cell renewal processes in the human hematopoietic system are believed to be supported by a small population of hematopoietic stem cells. 1,2 A major challenge in stem cell research is the establishment of culture systems that facilitate in vitro maintenance and augmentation of stem cell activity. This is of great impor- tance not only for hematopoietic stem cell transplantation but also for gene therapy. It is also an important step towards cellular and molecular understanding of the regulatory mechanisms that mediate the commitment vs self-renewal decision of stem cells.In the last two decades a number of hematopoietic cytokines have been identified. Studies using recombinant hematopoietic cytokines have revealed that hematopoietic cytokines alone or in combination support the proliferation of hematopoietic progenitors and that it is possible to increase the number of progenitors in culture. 3 Recently, several groups of investigators have reported that combinations of early-acting cytokines support the maintenance or expansion of progenitors and even of transplantable stem cells in both murine 4-7 and human systems. [8][9][10][11] On the other hand, however, the importance of stromal cells in the maintenance and expansion of primitive progenitors including transplantable stem cells have been repeatedly demonstrated. 12-20 A number of stromal cell lines have been reported to support the proliferation of human primitive progenitors. MS-5 is one such cell line, derived from noninfected Dexter-type murine long-term marrow culture, which supports CFU-S maintenance 21 and acts synergistically with human growth factors to stimulate the formation of blast colonies and macroscopic colonies from CD34 + CD38 Ϫ primitive progenitors in short-term methylcellulose assays. 22 MS-5, alone 23 or in combination with SCF and G-CSF, 24,25 also supports the proliferation of primitive lymphohematopoietic progenitors that are capable of differentiation along both myeloerythroid and lymphoid lineages and their differentiation toward the B cell lineage. Furthermore, a recent study has demonstrated that, in the presence of multiple cytokines, MS-5 cells promote a net increase of LTC-IC through self-renewal divisions. 26 In the present study, we demonstrate synergistic effects of MS-5 cells and early-acting hematopoietic growth factors, FL and TPO on the expansion of human cord blood CD34 + cells, CD34 + CD38 Ϫ cells, CFU-C and CAFC. Using an SRC assay we also found that transplantable stem cell activity was slightly augmented or at least maintained in our culture system.

show abstract

Rapid ex vivo expansion of human umbilical cord hematopoietic progenitors using a novel culture system

Cited by 117 publications

References 29 publications

Murine Stromal Cell Line HESS‐5 Maintains Reconstituting Ability of Ex Vivo‐Generated Hematopoietic Stem Cells from Human Bone Marrow and Cytokine‐Mobilized Peripheral Blood

Murine Stromal Cell Line HESS‐5 Maintains Reconstituting Ability of Ex Vivo‐Generated Hematopoietic Stem Cells from Human Bone Marrow and Cytokine‐Mobilized Peripheral Blood

Stromal support augments extended long-term ex vivo expansion of hemopoietic progenitor cells

Stromal cell-dependent ex vivo expansion of human cord blood progenitors and augmentation of transplantable stem cell activity

Contact Info

Product

Resources

About