Rapid evolution and horizontal gene transfer in the genome of a male-killingWolbachia

Abstract

Wolbachia are widespread bacterial endosymbionts that infect a large proportion of insect species. While some strains of this bacteria do not cause observable host phenotypes, many strains of Wolbachia have some striking effects on their hosts. In some cases, these symbionts manipulate host reproduction to increase the fitness of infected, transmitting females. Here we examine the genome and population genomics of a male-killing Wolbachia strain, wInn, that infects Drosophila innubila mushroom-feeding flies. We compared wInn to other closely-related Wolbachia genomes to understand the evolutionary dynamics of specific genes. The wInn genome is similar in overall gene content to wMel, but also contains many unique genes and repetitive elements that indicate distinct gene transfers between wInn and non-Drosophila hosts. We also find that genes in the Wolbachia prophage and Octomom regions are particularly rapidly evolving, including those putatively or empirically confirmed to be involved in host pathogenicity. Of the genes that rapidly evolve, many also show evidence of recent horizontal transfer among Wolbachia symbiont genomes, suggesting frequent movement of rapidly evolving regions among individuals. These dynamics of rapid evolution and horizontal gene transfer across the genomes of several Wolbachia strains and divergent host species may be important underlying factors in Wolbachia’s global success as a symbiont.

Forward genetics in Wolbachia: Regulation of Wolbachia proliferation by the amplification and deletion of an addictive genomic island

“…The Octomom region has the properties of a genomic island: it is not part of the Wolbachia core genome, since many Wolbachia strains lack this region, and seems to be horizontally transferred between Wolbachia strains [ 16 , 38 , 39 ]. Although the w Mel strain can lose this region and remain viable in laboratory conditions (here and [ 25 , 34 ]), natural variants of w Mel without Octomom are not known [ 40 ].…”

Forward genetics inWolbachia: Regulation ofWolbachiaproliferation by the amplification and deletion of an addictive genomic island

“…The Octomom region has the properties of a genomic island: it is not part of the Wolbachia core genome, since many Wolbachia strains lack this region, and seems to be horizontally transferred between Wolbachia strains [16,37,38]. Although the w Mel strain can lose this region and remain viable in laboratory conditions (here and [25,33]), natural variants of w Mel without Octomom are not known [39].…”

Male-killing-associated bacteriophage WO identified from comparisons of Wolbachia endosymbionts of Homona magnanima

“…Like the CI machinery of Wolbachia , the tandemly arrayed wmk genes might have combined effects, wherein one wmk shows toxicity toward both sexes and the other wmk acts as its suppressor. MK is considered to trigger diversification of the host sex determination pathways (10-11, 40-41). These systems in insects are diverse; for instance, the female-heterogametic system of Lepidoptera (including H. magnanima ) and the male-heterogametic system of Diptera (including D. melanogaster ) do not share any sex-determining genes known to date, except for the widely conserved doublesex gene.…”