Rapid evaporative ionization mass spectrometry for high-throughput screening in food analysis: The case of boar taint

Abstract

Boar taint is a contemporary off-odor present in meat of uncastrated male pigs. As European Member States intend to abandon surgical castration of pigs by 2018, this off-odor has gained a lot of research interest. In this study, rapid evaporative ionization mass spectrometry (REIMS) was explored for the rapid detection of boar taint in neck fat. Untargeted screening of samples (n=150) enabled discrimination between sow, tainted and untainted boars. The obtained OPLS-DA models showed excellent classification accuracy, i.e. 99% and 100% for sow and boar samples or solely boar samples, respectively. Furthermore, the obtained models demonstrated excellent validation characteristics (R(Y)=0.872-0.969; Q(Y)=0.756-0.917), which were confirmed by CV-ANOVA (p<0.001) and permutation testing. In conclusion, in this work for the first time highly accurate and high-throughput (<10s) classification of tainted and untainted boar samples was achieved, rendering REIMS a promising technique for predictive modelling in food safety and quality applications.

Food authentication in real life: How to link nontargeted approaches with routine analytics?

“…Laser systems working with different wavelengths can be used to ablate large biomolecules, such as DNA or proteins, as well as smaller metabolites and elements [32,33]. Some analytical platforms also enable the direct nontargeted measurement of food samples without any extraction, for example by means of infrared (IR) spectroscopy [34] (near IR [NIR] [35], mid IR [36]), Raman spectroscopy [37]) as well as different ionization sources for MS: direct analysis in real time (DART) [38], desorption electrospray ionization (DESI), rapid evaporative ionization mass spectrometry (REIMS) [39], liquid extraction surface analysis (LESA) [40], or laser ablation electrospray ionization (LAESI). However, the analytical information content (i.e.…”

Exploration of Rapid Evaporative-Ionization Mass Spectrometry as a Shotgun Approach for the Comprehensive Characterization of Kigelia Africana (Lam) Benth. Fruit

“…To the best of our knowledge, this is the first work dealing with the untargeted characterization of a botanical species by the novel REIMS technology, taking also into account that the majority of the applications reported in the literature focus on animals [17,[20][21][22][23][24][25][26] and microorganisms [27][28][29][30], apart from several studies involving human cancer [14,15,18,20,31,32].…”

“…Taking into account the novelty of the REIMS ionization source, which most commonly focused on lipid compounds [13][14][15][16][17][18][20][21][22][23][24][25][26]31,32], some additional experiments were necessary to confirm the identification of these phenols. In particular, the polyphenol isoquercetrin, available as pure standard, was infused by the syringe pump into the REIMS source to elucidate the ionization mechanism in positive polarity.…”

Meet interesting abbreviations in clinical mass spectrometry: from compound classification by REIMS to multimodal and mass spectrometry imaging (MSI)

“…Powerful ability of REIMS tissue characterization and differentiation is used for several non-clinical applications such as food quality control studies and in research studies in microbiology and animal research. Possible adulteration of meat products can also be easily detected by REIMS (Verplanken et al, 2017). The characterization of meat originating from different species (horse, cattle, and venison) was demonstrated with high accuracy using mixed patties with a 5% detection limit (Balog et al, 2016).…”