Rapid evaluation of bioactive Ti-based surfaces using an in vitro titration method

Abstract

The prediction of implant behavior in vivo by the use of easy-to-perform in vitro methods is of great interest in biomaterials research. Simulated body fluids (SBFs) have been proposed and widely used to evaluate the bone-bonding ability of implant materials. In view of its limitations, we report here a rapid in vitro method based on calcium titration for the evaluation of in vivo bioactivity. Using four different titanium surfaces, this method identifies that alkaline treatment is the key process to confer bioactivity to titanium whereas no significant effect from heat treatment is observed. The presence of bioactive titanium surfaces in the solution during calcium titration induces an earlier nucleation of crystalline calcium phosphates and changes the crystallization pathway. The conclusions from this method are also supported by the standard SBF test (ISO 23317), in vitro cell culture tests using osteoblasts and in vivo animal experiments employing a pelvic sheep model.

Micropatterning Decellularized ECM as a Bioactive Surface to Guide Cell Alignment, Proliferation, and Migration

“…A significant portion of tissue engineering research focuses on the ability to derive native biological structures as biomaterials for regenerative purposes [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ]. Biomimetic structures, in particular, strive to recreate naturally regenerative microenvironments to promote desired cell behavior and mitigate inflammatory responses [ 9 ].…”

Development of methods for detecting the fate of mesenchymal stem cells regulated by bone bioactive materials

“…The fate of MSCs can be regulated by bioactive materials to promote their adhesion, proliferation and directional differentiation, so as to achieve optimistical tissue regeneration [ 2 ]. Orthopaedic implants have undergone a development from inert biomaterials, which lack the ability to interact with physiological tissues [ 3 ], to bioactive materials, which have the ability to regulate the fate of MSCs to regenerate damaged tissue [ [4] , [5] , [6] , [7] , [8] , [9] ]. The biological properties of bioactive materials are determined by their structure and physicochemical properties [ [10] , [11] , [12] ].…”

Characterization of the in Vitro Osteogenic Response to Submicron TiO2 Particles of Varying Structure and Crystallinity

“…The formation of a superficial bonelike apatite layer after coming in contact with the body fluid is regarded as one of the main features of bioactive biomaterials. 36 This mineral layer decreases the surface free energy at the biomaterial/bone interface and favors the binding between the implant and the living tissue. 37 This is, therefore, an essential requirement to hamper the formation of a fibrous tissue encapsulating and isolating the implanted material from the host bone, which would lead to implant failure.…”