Rapid Epithelial Restitution in the Stomach: An Updated Perspective

Lacy, Eric R.

doi:10.3109/00365529509090260

Cited by 24 publications

(21 citation statements)

References 14 publications

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“…Previous studies have shown that cultured endometrial epithelia exhibit a basal I SC that can be effectively blocked by amiloride, indicative of predominant Na ϩ absorption [10,13]. We recently found that the ENaC activity of endometrial cultures could be affected by coating the permeable support with Matrigel [14], which resembles the extracellular matrix facilitating rapid epithelial reconstitution and differentiation [15,16]. As shown in Fig.…”

Section: Introductionmentioning

confidence: 92%

Enhanced Epithelial Na+ Channel (ENaC) Activity in Mouse Endometrial Epithelium by Upregulation of .GAMMA.ENaC Subunit.

Tsang

Chan²,

Wang³

et al. 2001

JJP

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Section: Introductionmentioning

confidence: 92%

Enhanced Epithelial Na+ Channel (ENaC) Activity in Mouse Endometrial Epithelium by Upregulation of .GAMMA.ENaC Subunit.

Tsang

Chan²,

Wang³

et al. 2001

JJP

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“…This fundamental early repair process can begin within minutes, well before extensive infl ammatory processes occur, and has been termed 'restitution' [25]. It is typical of the GI mucosa (for reviews, see [25][26][27][28][29][30], the respiratory tract [31][32][33][34], the urothelium [35], the gall bladder epithelium [36], the oral epithelium [37] and the cornea [38]. For example, surface mucous cells migrate in the stomach (starting within 3 min after injury), whereas enterocytes (not goblet cells) participate in duodenal restitution with a fi rst sign of repair after 3 h [26].…”

Section: Mucosal Restitutionmentioning

confidence: 99%

Trefoil factors

Hoffmann

2005

Cell. Mol. Life Sci.

184

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Rapid repair of mucous epithelia is essential for preventing inflammation which is a critical component of cancer progression. 'Restitution' is an early repair process which can begin within minutes and is achieved via the migration of neighbouring cells into the wounded area. Mucosal restitution is a multistep process which requires continuous blood flow and includes at least (i) the reduction of cell-cell contacts and a shift in the cell shape towards a migratory phenotype (characteristics of the epithelial-mesenchymal transition), (ii) migration of cells, (iii) repolarization and formation of tight junctions (morphological restitution) and (iv) restoration of barrier function (transmucosal epithelial resistance, functional restitution). Secretory TFF (trefoil factor family) peptides TFF1, TFF2 and TFF3 are well known for their potent protective and healing effects after mucosal damage (function as 'luminal surveillance peptides'). Here, the contributions of the TFFs during the different steps of mucosal restitution are discussed, i. e. the modulation of cell-cell contacts, their motogenic activity and synergy with epidermal growth factor, their anti-apoptotic and pro-angiogenic effects. Special emphasis has been given to discussion of the various signal transduction networks triggered by TFFs. It is becoming increasingly clear that these pathways differ depending on the respective TFF.

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“…Observations have been reported on airway epithelial cells that different culture conditions, such as different culture substrates (plastic vs filter) and hormone treatments, give rise to differential expression of ENaC (18). We tried to define the endometrial cultures using Matrigel which resembles the extracellular matrix facilitating rapid epithelial reconstitution and differentiation (19,20). The amiloride sensitivity of endometrial cells cultured on different permeable supports, i.e., nonMatrigel-coated, single-and double-Matrigel-coated, was examined and compared.…”

Section: Effect Of Matrigel On Enac and Cftr Expressionmentioning

confidence: 99%