Rapid enzyme system for the identification of pathogenic Neisseria spp

Brown, J D; Thomas, Karen R.

doi:10.1128/jcm.21.5.857-858.1985

Cited by 18 publications

(5 citation statements)

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“…With the RIM-N test, N. cinerea isolates as well as three N. subflava (the sucrose-positive biovar formerly designated N. perfiava) isolates produced weak positive reactions (or- (3) 100.0 100.0 100.0 66.7/66.7 100.0 100.0 B. catarrhalis (9) 100.0 100.0 100.0 100.0 100.0 66.7/66.7 N. sicca (4) 100.0 100.0 0/0 100.0 100.0 oloa N. cinerea (4) o.oa 1oo.oa o/oa 10.Oa 10.Oa ooa N. subflavab (5) 100.0 10.O< 40.0/40.0 40/60.0 80.0/1j0a 0/0a Others (4) 100.0a 100.0a 100.0a 100.0a 100.0a oIoa aManufacturers make no claim to identify species.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

1988

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The performance of eight methods in identifying Neisseria species, particularly N. gonorrhoeae, was evaluated. These methods included four rapid carbohydrate utilization tests (Gonobio-Test, Neisseria-Kwik, RIM-N, and Minitek); the Gonochek 11, a test which is based on the utilization of chromogenic substrates; and three monoclonal antibody tests (Syva MicroTrak, GonoGen, and Phadebact Monoclonal GC OMNI Test). In all, 182 isolates comprised in six species of Neisseria as well as Branhamella catarrhalis and Moraxella sp. were tested. Cystine-tryptic digest agar supplemented with sugars was included for reference purposes. In the carbohydrate utilization tests, the sensitivity and specificity of the Neisseria-Kwik and Minitek tests for the identification of N. gonorrhoeae were 100%. This compared with sensitivities and specificities, respectively, of 100 and 99.1% for the Gonobio-Test and 99.1 and 100% for cystine-tryptic digest agar sugars and the RIM-N test. The sensitivity and specificity of the Gonochek II test were 99.0 and 86.7%, respectively. Although most test kits did not claim to identify all Neisseria species, in several cases isolates of N. subflava were misidentified or could be misinterpreted as N. gonorrhoeae or N. meningitidis. With the monoclonal reagents, the Syva MicroTrak system was 100% sensitive and 100% specific. The GonoGen test was both 99.1% sensitive and specific, while the Phadebact Monoclonal GC OMNI Test was 99.1% sensitive but 91.2% specific. With this latter test, cross-reactions were observed with strains of B. catarrhalis, N. cinerea, and N. lactamica.

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Section: Resultsmentioning

confidence: 99%

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

1988

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“…Rapid identification of Neisseria gonorrhoeae may be accomplished by several diverse methods. The methods include carbohydrate utilization with preformed enzymes or enzymes formed during growth (5,8,12,15,20) and immunological (4,7,10,13), chromogenic (2,19), and radiometric (16) procedures. Immunological procedures include coagglutination with polyclonal (11) (Phadebact Gonococcus Test; Pharmacia Diagnostics, Piscataway, N.J.) and murine monoclonal (9) (Gono Gen; New Horizons Diagnostics, Columbia, Md.)…”

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confidence: 99%

Fluorescent monoclonal antibody compared with carbohydrate utilization for rapid identification of Neisseria gonorrhoeae

Welch

Cartwright

1988

J Clin Microbiol

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A commercially available fluorescein-conjugated monoclonal antibody (MAb) (Syva Co., Palo Alto, Calif.; Genetic Systems, Seattle, Wash.) against Neisseria gonorrhoeae was compared with a standard cystine Trypticase agar (CTA) sugar utilization method and with three rapid carbohydrate utilization tests, including the Minitek (BBL Microbiology Systems, Cockeysville, Md.), Neisseria-Stat (Richardson Scientific, Dallas, Tex.), and Neisseria-Kwik (Micro-Biologics, St. Cloud, Minn.) systems for the identification of Neisseria species. The MAb correctly identified ail 86 clinical isolates of N. gonorrhoeae. Of these 86 isolates, 28 were found later (48 h after the initial inoculation) to be contaminated with non-Neisseria bacteria. In the other four test systems studied, the identification rates for pure and contaminated N. gonorrhoeae cultures were, respectively, as follows: CTA sugars, 88 and 32%; Minitek, 67 and 50%; Neisseria-Stat, 97 and 96%; and Neisseria-Kwik, 80 and 74%. The MAb did not identify any of the 50 nongonoccocal Neisseria isolates tested. The most expensive test system was the MAb, followed by the Neisseria-Kwik, Minitek, Neisseria-Stat, and CTA sugars systems. The MAb appears to be a rapid and accurate method to identify in vitro isolates of N. gonorrhoeae.

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“…Unlike Gonochek Il and Identicult-Neisseria, which rely solely on the detection of prolyl aminopeptidase for identifying N. gonorrhoeae (3,11,12,14,15,25), the PRO (prolyl aminopeptidase) substrate in the HNID panel is hydrolyzed not only by N. gonorrhoeae and N. cinerea, but also by B. catarrhalis. In the other systems, B. catarrhalis strains are prolyl aminopeptidase negative, and the absence of this and other enzymatic activities provides a presumptive identification of B. catarrhalis (3,11,12,14,15). The HNID panel relies on NO3 and NO2 reduction and the negative GLU reaction to identify B. catarrhalis, and indeed, all isolates of this species were uniform in these characteristics ( Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…Table 1 shows the results obtained by using the HNID panel for the identification of Neisseria spp., B. catarrhalis, and G. vaginalis. Of 86 gonococcal strains, 3 Six biotype I, eight biotype Il, and one biotype V H. influenza strains and one biotype I H. parainfluenzae strain were P-lactamase positive. All of these strains were detected by the BL test on the HNID panel.…”

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confidence: 99%

Identification of Neisseria spp., Haemophilus spp., and other fastidious gram-negative bacteria with the MicroScan Haemophilus-Neisseria identification panel

1989

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The Haemophilus-Neisseria identification (HNID) panel (American MicroScan, Sacramento, Calif.) is a 4-h microdilution format system for identification of Haemophilus and Neisseria spp., Branhamella (Moraxella) catarrhalis, and Gardnerella vaginalis. The HNID panel was evaluated by using 423 clinical isolates and stock strains of these organisms, and HNID identifications were compared with those obtained by conventional methods. In addition, 32 isolates representing six genera not included in the HNID data base were tested to determine whether these organisms would produce unique biotype numbers for possible inclusion in the data base. The HNID panel correctly identified 95.3% of 86 Neisseria gonorrhoeae strains, 96% of 25 G. vaginalis strains, and 100% of 28 Neisseria lactamica strains and 48 B. catarrhalis strains. Only 64.7% of 68 Neisseria meningitidis isolates were identified correctly owing to false-negative or equivocal carbohydrate and/or aminopeptidase reactions. Among the Haemophilus spp., 98.8% of 83 H. influenza strains, 97.1% of 34 H. parainfluenzae strains, and 80% of 15 H. aphrophilus and H. paraphrophilus strains were correctly identified. Eight strains of Neisseria cinerea, a species not included in the data base, produced profiles identical with those for B. catarrhalis and N. gonorrhoeae. Isolates of other species not included in the data base, including Eikenella corrodens, Kingella spp., and Cardiobacterium hominis, produced unique biochemical reaction patterns on the panel. Modification of interpretative criteria for certain tests, expansion of the data base to include other species, and suggestions for additional confirmatory tests will increase the accuracy and utility of the HNID panel.

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Rapid enzyme system for the identification of pathogenic Neisseria spp

Cited by 18 publications

References 0 publications

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

Fluorescent monoclonal antibody compared with carbohydrate utilization for rapid identification of Neisseria gonorrhoeae

Identification of Neisseria spp., Haemophilus spp., and other fastidious gram-negative bacteria with the MicroScan Haemophilus-Neisseria identification panel

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