Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactor

Kan'no, Ken‐ichi; Maeda, Hideaki; Izumo, S.; Ikuno, M.; Takeshita, Kazuhito; Tashiro, Asuka; Fujii, Masayuki

doi:10.1039/b108741j

Cited by 67 publications

(38 citation statements)

References 16 publications

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“…Numerous micro enzyme-reactors have been developed because they are superior in reducing the reaction time and use minimal amount of reagents. Therefore, these immobilized microfluidic enzyme reactors have also increasingly applied in biosensors [13], and organic or enzymatic synthesis [14][15][16].…”

Section: Application Of Microfluidic Enzymatic Reactors or Proteomicsmentioning

confidence: 99%

Microfluidic enzymatic reactors for proteome research

Liu

Yang

2007

Anal Bioanal Chem

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Section: Application Of Microfluidic Enzymatic Reactors or Proteomicsmentioning

confidence: 99%

Microfluidic enzymatic reactors for proteome research

Liu

Yang

2007

Anal Bioanal Chem

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“…Decreasing effective reaction times when using microstructured reactors have also been described for sucrochemistry; the hydrolysis of p-nitrophenyl-b-D-galactopyranoside and the transgalactolysation of p-nitrophenyl-2-acetamid-2-deoxy-b-D-glucopyranoside in a micro reactor were faster compared to the use of commercial test tubes [17]. This is most likely caused by the better mixing efficiency in the microstructured reactor.…”

Section: Reduction Of Reaction Timesmentioning

confidence: 94%

Organic Synthesis with Microstructured Reactors

Hessel¹,

Löwe²

2005

Chem Eng & Technol

102

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This article describes the opportunities microstructured reactors offer for chemical plant engineering. This suitability for chemical production is commonly regarded to be the key to market penetration. Century-long practices with batch-type stirring and, correspondingly, the existence of capital-intensive large-scale plants, hinder an easy and fast implementation of chemical micro process engineering. Seen in the long term, there is potential that new plants can be equipped with microstructured reactors. Only economic balances, however, which draw up profitability, will open the door to the usage of chemical micro process engineering for plant construction. The main arguments for using microstructured reactors are enhanced conversion and selectivity, increased space-time yields, waste reduction and better safety given the small reactor volumes. Credit-card sized reaction systems allow one to perform the screening of multi-step reactions in one run. Even more impressively, similar screening is carried out for single-step reactions faster and more reliably. Moreover, safe processing with microstructured reactors in the explosive regime enlarges the traditional range of processing. The reaction guidance by microstructured reactors can further influence subsequent processing steps, such as product purification, and in this way can lower the energy costs of processes.

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“…β-galactosidase Poly(methylmethacrylate) Enzymatic hydrolysis of p-nitrophenyl-β-D-galactopyranoside is five times faster than in batch. The inverse reaction of transglycosylation is also enhanced [87]. Laccase Glass microchannels Enzymatic degradation of p-chlorophenol is carried out in a two-phase system with different flow rate and channel shape [76].…”

Section: Glass Microchannelsmentioning

confidence: 99%

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Laurenti¹,

Vianna²

2016

Biocatalysis

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Microfluidic reaction devices are a very promising technology for chemical and biochemical processes. In microreactors, the micro dimensions, coupled with a high surface area/volume ratio, permit rapid heat exchange and mass transfer, resulting in higher reaction yields and reaction rates than in conventional reactors. Moreover, the lower energy consumption and easier separation of products permit these systems to have a lower environmental impact compared to macroscale, conventional reactors. Due to these benefits, the use of microreactors is increasing in the biocatalysis field, both by using enzymes in solution and their immobilized counterparts. Following an introduction to the most common applications of microreactors in chemical processes, a broad overview will be given of the latest applications in biocatalytic processes performed in microreactors with free or immobilized enzymes. In particular, attention is given to the nature of the materials used as a support for the enzymes and the strategies employed for their immobilization. Mathematical and engineering aspects concerning fluid dynamics in microreactors were also taken into account as fundamental factors for the optimization of these systems.

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Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactor

Cited by 67 publications

References 16 publications

Microfluidic enzymatic reactors for proteome research

Microfluidic enzymatic reactors for proteome research

Organic Synthesis with Microstructured Reactors

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

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